Datasets:

huy-nh-2000

/

instructUIE

like 0

[]

{ "id": "2800", "sentence": "Octamer independent activation of transcription from the kappa immunoglobulin germline promoter .", "label": " ( DNA: kappa immunoglobulin germline promoter, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: kappa immunoglobulin germline promoter, None) " }

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{ "id": "2801", "sentence": "Previous analyses of immunoglobulin V region promoters has led to the discovery of a common octamer motif which is functionally important in the tissue-specific and developmentally regulated transcriptional activation of immunoglobulin genes .", "label": " ( DNA: immunoglobulin genes, None) , ( DNA: octamer motif, None) , ( DNA: immunoglobulin V region promoters, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: immunoglobulin genes, None) , ( DNA: octamer motif, None) , ( DNA: immunoglobulin V region promoters, None) " }

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{ "id": "2802", "sentence": "The germline promoters ( Ko ) located upstream of the J region gene segments of the kappa locus also contain an octamer motif (containing a single base pair mutation and referred to as the variant octamer) which has been shown previously to bind Oct-1 and Oct-2 transcription factors in vitro.", "label": " ( DNA: kappa locus, None) , ( DNA: germline promoters, None) , ( DNA: J region gene segments, None) , ( protein: Oct-2, None) , ( protein: Oct-1, None) , ( DNA: base pair mutation, None) , ( protein: transcription factors, None) , ( DNA: Ko, None) , ( DNA: octamer motif, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: kappa locus, None) , ( DNA: germline promoters, None) , ( DNA: J region gene segments, None) , ( protein: Oct-2, None) , ( protein: Oct-1, None) , ( DNA: base pair mutation, None) , ( protein: transcription factors, None) , ( DNA: Ko, None) , ( DNA: octamer motif, None) " }

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{ "id": "2803", "sentence": "To further elucidate the role of this variant octamer motif in the regulation of germline transcription from the unrearranged kappa locus , we have quantitated the relative binding affinity of Oct-1 and Oct-2 for the variant octamer motif and determined the functional role of this octamer motif in transcriptional activation .", "label": " ( DNA: germline, None) , ( protein: Oct-1, None) , ( DNA: octamer motif, None) , ( DNA: octamer motif, None) , ( protein: Oct-2, None) , ( DNA: kappa locus, None) , ( DNA: octamer motif, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: germline, None) , ( protein: Oct-1, None) , ( DNA: octamer motif, None) , ( DNA: octamer motif, None) , ( protein: Oct-2, None) , ( DNA: kappa locus, None) , ( DNA: octamer motif, None) " }

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{ "id": "2804", "sentence": "We find that, although the variant octamer motif binds Oct-1 and Oct-2 in vitro with 5-fold lower affinity than the consensus octamer motif , mutation of the variant octamer motif to either a consensus octamer or non- octamer motif has no effect on transcriptional activation from the germline promoter .", "label": " ( protein: Oct-2, None) , ( DNA: non- octamer motif, None) , ( DNA: germline promoter, None) , ( DNA: octamer motif, None) , ( DNA: octamer motif, None) , ( DNA: consensus octamer motif, None) , ( DNA: octamer motif, None) , ( DNA: consensus octamer, None) , ( protein: Oct-1, None) , ( DNA: octamer motif, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: Oct-2, None) , ( DNA: non- octamer motif, None) , ( DNA: germline promoter, None) , ( DNA: octamer motif, None) , ( DNA: octamer motif, None) , ( DNA: consensus octamer motif, None) , ( DNA: octamer motif, None) , ( DNA: consensus octamer, None) , ( protein: Oct-1, None) , ( DNA: octamer motif, None) " }

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{ "id": "2805", "sentence": "We also find significant differences in activation of germline and V region promoters by kappa enhancers .", "label": " ( DNA: kappa enhancers, None) , ( DNA: germline, None) , ( DNA: V region promoters, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: kappa enhancers, None) , ( DNA: germline, None) , ( DNA: V region promoters, None) " }

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{ "id": "2806", "sentence": "Our results suggest that the germline promoters and V region promoters differ in their dependence on octamer for activation and respond differently to enhancer activation .", "label": " ( DNA: V region promoters, None) , ( DNA: octamer, None) , ( DNA: germline promoters, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: V region promoters, None) , ( DNA: octamer, None) , ( DNA: germline promoters, None) " }

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{ "id": "2807", "sentence": "These findings have important implications in regulation of germline transcription as well as concomitant activation of the V-J recombination of the kappa light chain locus .", "label": " ( DNA: kappa light chain locus, None) , ( DNA: germline, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: kappa light chain locus, None) , ( DNA: germline, None) " }

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{ "id": "2808", "sentence": "Generation of CD1+RelB+ dendritic cells and tartrate-resistant acid phosphatase-positive osteoclast-like multinucleated giant cells from human monocytes .", "label": " ( cell type: human monocytes, None) , ( cell type: tartrate-resistant acid phosphatase-positive osteoclast-like multinucleated giant cells, None) , ( cell type: CD1+RelB+ dendritic cells, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell type: human monocytes, None) , ( cell type: tartrate-resistant acid phosphatase-positive osteoclast-like multinucleated giant cells, None) , ( cell type: CD1+RelB+ dendritic cells, None) " }

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{ "id": "2809", "sentence": "We previously showed that granulocyte-macrophage colony-stimulating factor ( GM-CSF ) and macrophage colony-stimulating factor ( M-CSF ) stimulate the differentiation of human monocytes into two phenotypically distinct types of macrophages .", "label": " ( protein: M-CSF, None) , ( protein: macrophage colony-stimulating factor, None) , ( protein: granulocyte-macrophage colony-stimulating factor, None) , ( protein: GM-CSF, None) , ( cell type: human monocytes, None) , ( cell type: macrophages, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: M-CSF, None) , ( protein: macrophage colony-stimulating factor, None) , ( protein: granulocyte-macrophage colony-stimulating factor, None) , ( protein: GM-CSF, None) , ( cell type: human monocytes, None) , ( cell type: macrophages, None) " }

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{ "id": "2810", "sentence": "However, in vivo, not only CSF but also many other cytokines are produced under various conditions.", "label": " ( protein: CSF, None) , ( protein: cytokines, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: CSF, None) , ( protein: cytokines, None) " }

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{ "id": "2811", "sentence": "Those cytokines may modulate the differentiation of monocytes by CSFs .", "label": " ( cell type: monocytes, None) , ( protein: cytokines, None) , ( protein: CSFs, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell type: monocytes, None) , ( protein: cytokines, None) , ( protein: CSFs, None) " }

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{ "id": "2812", "sentence": "In the present study, we showed that CD14+ adherent human monocytes can differentiate into CD1+relB+ dendritic cells ( DC ) by the combination of GM-CSF plus interleukin-4 ( IL-4 ) and that they differentiate into tartrate-resistant acid phosphatase (TRAP)-positive osteoclast-like multinucleated giant cells ( MGC ) by the combination of M-CSF plus IL-4 .", "label": " ( cell type: MGC, None) , ( cell type: CD1+relB+ dendritic cells, None) , ( cell type: human monocytes, None) , ( cell type: tartrate-resistant acid phosphatase (TRAP)-positive osteoclast-like multinucleated giant cells, None) , ( protein: M-CSF, None) , ( protein: interleukin-4, None) , ( protein: IL-4, None) , ( cell type: CD14+ adherent human monocytes, None) , ( cell type: DC, None) , ( protein: GM-CSF, None) , ( protein: IL-4, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell type: MGC, None) , ( cell type: CD1+relB+ dendritic cells, None) , ( cell type: human monocytes, None) , ( cell type: tartrate-resistant acid phosphatase (TRAP)-positive osteoclast-like multinucleated giant cells, None) , ( protein: M-CSF, None) , ( protein: interleukin-4, None) , ( protein: IL-4, None) , ( cell type: CD14+ adherent human monocytes, None) , ( cell type: DC, None) , ( protein: GM-CSF, None) , ( protein: IL-4, None) " }

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{ "id": "2813", "sentence": "However, the monocyte-derived DC were not terminally differentiated cells ; they could still convert to macrophages in response to M-CSF .", "label": " ( protein: M-CSF, None) , ( cell type: DC, None) , ( cell line: terminally differentiated cells, None) , ( cell type: macrophages, None) , ( cell line: monocyte-derived DC, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: M-CSF, None) , ( cell type: DC, None) , ( cell line: terminally differentiated cells, None) , ( cell type: macrophages, None) , ( cell line: monocyte-derived DC, None) " }

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{ "id": "2814", "sentence": "Tumor necrosis factor-alpha ( TNF-alpha ) stimulated the terminal differentiation of the DC by downregulating the expression of the M-CSF receptor , cfms mRNA , and aborting the potential to convert to macrophages .", "label": " ( protein: M-CSF, None) , ( cell type: DC, None) , ( protein: Tumor necrosis factor-alpha, None) , ( protein: TNF-alpha, None) , ( protein: M-CSF receptor, None) , ( cell type: macrophages, None) , ( RNA: cfms mRNA, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: M-CSF, None) , ( cell type: DC, None) , ( protein: Tumor necrosis factor-alpha, None) , ( protein: TNF-alpha, None) , ( protein: M-CSF receptor, None) , ( cell type: macrophages, None) , ( RNA: cfms mRNA, None) " }

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{ "id": "2815", "sentence": "In contrast to IL-4 , interferon-gamma ( IFN-gamma ) had no demonstrable effect on the differentiation of monocytes .", "label": " ( protein: interferon-gamma, None) , ( protein: IL-4, None) , ( protein: IFN-gamma, None) , ( cell type: monocytes, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: interferon-gamma, None) , ( protein: IL-4, None) , ( protein: IFN-gamma, None) , ( cell type: monocytes, None) " }

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{ "id": "2816", "sentence": "Rather, IFN-gamma antagonized the effect of IL-4 and suppressed the DC and MGC formation induced by GM-CSF + IL-4 and M-CSF + IL-4 , respectively.", "label": " ( protein: GM-CSF, None) , ( cell type: DC, None) , ( protein: IL-4, None) , ( protein: IL-4, None) , ( cell type: MGC, None) , ( protein: IFN-gamma, None) , ( protein: M-CSF, None) , ( protein: IL-4, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: GM-CSF, None) , ( cell type: DC, None) , ( protein: IL-4, None) , ( protein: IL-4, None) , ( cell type: MGC, None) , ( protein: IFN-gamma, None) , ( protein: M-CSF, None) , ( protein: IL-4, None) " }

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{ "id": "2817", "sentence": "Taken together, these results provide a new aspect to our knowledge of monocyte differentiation and provide evidence that human monocytes are flexible in their differentiation potential and are precursors not only of macrophages but also of CD1+relB+DC and TRAP-positive MGC .", "label": " ( cell type: human monocytes, None) , ( cell type: macrophages, None) , ( cell type: TRAP-positive MGC, None) , ( cell type: monocytes, None) , ( cell type: CD1+relB+DC, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell type: human monocytes, None) , ( cell type: macrophages, None) , ( cell type: TRAP-positive MGC, None) , ( cell type: monocytes, None) , ( cell type: CD1+relB+DC, None) " }

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{ "id": "2818", "sentence": "Such a diverse pathway of monocyte differentiation may constitute one of the basic mechanisms of immune regulation .", "label": " None", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " None" }

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{ "id": "2819", "sentence": "The NF-kappa B inhibitor , tepoxalin, suppresses surface expression of the cell adhesion molecules CD62E , CD11b/CD18 and CD106 .", "label": " ( protein: CD11b/CD18, None) , ( protein: NF-kappa B, None) , ( protein: CD62E, None) , ( protein: cell adhesion molecules, None) , ( protein: CD106, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: CD11b/CD18, None) , ( protein: NF-kappa B, None) , ( protein: CD62E, None) , ( protein: cell adhesion molecules, None) , ( protein: CD106, None) " }

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{ "id": "2820", "sentence": "Tepoxalin , a dual enzyme inhibitor of cyclooxygenase and 5-lipoxygenase has been shown to inhibit T-cell activation .", "label": " ( protein: 5-lipoxygenase, None) , ( protein: cyclooxygenase, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: 5-lipoxygenase, None) , ( protein: cyclooxygenase, None) " }

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{ "id": "2821", "sentence": "Its immunosuppressive property is distinct from cyclosporin because only tepoxalin, but not cyclosporin , suppresses NF-kappa B activation .", "label": " ( protein: NF-kappa B, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: NF-kappa B, None) " }

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{ "id": "2822", "sentence": "Here we report that tepoxalin selectively inhibits intercellular adhesion molecule-1 ( ICAM-1 , CD54 )/MAC-1 ( CD11b/CD18 ) dependent adhesion of polymorphonuclear cells to IL-1 activated human umbilical vein endothelial cells .", "label": " ( protein: CD54, None) , ( protein: ( ICAM-1 , CD54 )/MAC-1, None) , ( protein: ICAM-1, None) , ( cell type: polymorphonuclear cells, None) , ( protein: CD11b/CD18, None) , ( protein: intercellular adhesion molecule-1, None) , ( cell type: human umbilical vein endothelial cells, None) , ( protein: IL-1, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: CD54, None) , ( protein: ( ICAM-1 , CD54 )/MAC-1, None) , ( protein: ICAM-1, None) , ( cell type: polymorphonuclear cells, None) , ( protein: CD11b/CD18, None) , ( protein: intercellular adhesion molecule-1, None) , ( cell type: human umbilical vein endothelial cells, None) , ( protein: IL-1, None) " }

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{ "id": "2823", "sentence": "The mechanism of inhibition is related to the surface expression of several cell adhesion molecules .", "label": " ( protein: adhesion molecules, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: adhesion molecules, None) " }

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{ "id": "2824", "sentence": "Flow cytometry analyses on cultured cells that were treated with tepoxalin or antisense oligonucleotides to the P65/p50 subunit of NF-kappa B , and then stimulated with PMA , revealed a reduced expression of CD11b/CD18 on monocytic HL60 cells , and endothelial adhesion molecule-1 ( CD62E ) and vascular adhesion molecule-1 ( CD106 ) on human umbilical vein endothelial cells .", "label": " ( protein: CD62E, None) , ( protein: vascular adhesion molecule-1, None) , ( cell type: human umbilical vein endothelial cells, None) , ( protein: endothelial adhesion molecule-1, None) , ( cell line: monocytic HL60 cells, None) , ( protein: NF-kappa B, None) , ( protein: CD11b/CD18, None) , ( protein: CD106, None) , ( protein: P65/p50 subunit, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: CD62E, None) , ( protein: vascular adhesion molecule-1, None) , ( cell type: human umbilical vein endothelial cells, None) , ( protein: endothelial adhesion molecule-1, None) , ( cell line: monocytic HL60 cells, None) , ( protein: NF-kappa B, None) , ( protein: CD11b/CD18, None) , ( protein: CD106, None) , ( protein: P65/p50 subunit, None) " }

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{ "id": "2825", "sentence": "Expression of other adhesion molecules such as lymphocyte function associated-antigen-1 ( CD11a /CD18 ) and CD54 were unaffected.", "label": " ( protein: CD54, None) , ( protein: adhesion molecules, None) , ( protein: CD11a, None) , ( protein: lymphocyte function associated-antigen-1, None) , ( protein: CD11a /CD18, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: CD54, None) , ( protein: adhesion molecules, None) , ( protein: CD11a, None) , ( protein: lymphocyte function associated-antigen-1, None) , ( protein: CD11a /CD18, None) " }

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{ "id": "2826", "sentence": "Tepoxalin also inhibited the secretion of a NF-kappa B regulated chemokine , IL-8 , a known inducer of CD11b/CD18 expression .", "label": " ( protein: CD11b/CD18, None) , ( protein: NF-kappa B regulated chemokine, None) , ( protein: IL-8, None) , ( protein: NF-kappa B, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: CD11b/CD18, None) , ( protein: NF-kappa B regulated chemokine, None) , ( protein: IL-8, None) , ( protein: NF-kappa B, None) " }

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{ "id": "2827", "sentence": "Thus the suppression of CD11b/CD18 expression by tepoxalin may involve IL-8 .", "label": " ( protein: IL-8, None) , ( protein: CD11b/CD18, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: IL-8, None) , ( protein: CD11b/CD18, None) " }

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{ "id": "2828", "sentence": "Our results suggest that by inhibiting NF-kappa B activation , surface expression of several adhesion molecules can be modulated and that tepoxalin may be useful in treating selected adhesion mediated events such as leukocyte migration or atherosclerotic plaque formation .", "label": " ( protein: NF-kappa B, None) , ( protein: adhesion molecules, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: NF-kappa B, None) , ( protein: adhesion molecules, None) " }

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{ "id": "2829", "sentence": "Characterization of a CD43/leukosialin -mediated pathway for inducing apoptosis in human T-lymphoblastoid cells .", "label": " ( protein: CD43/leukosialin, None) , ( cell type: human T-lymphoblastoid cells, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: CD43/leukosialin, None) , ( cell type: human T-lymphoblastoid cells, None) " }

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{ "id": "2830", "sentence": "The monoclonal antibody ( mAb ) J393 induces apoptosis in Jurkat T-cells .", "label": " ( protein: J393, None) , ( protein: mAb, None) , ( protein: monoclonal antibody, None) , ( cell line: Jurkat T-cells, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: J393, None) , ( protein: mAb, None) , ( protein: monoclonal antibody, None) , ( cell line: Jurkat T-cells, None) " }

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{ "id": "2831", "sentence": "NH2 -terminal amino acid sequence analysis identified the 140-kDa surface antigen for mAb J393 as CD43/leukosialin , the major sialoglycoprotein of leukocytes .", "label": " ( protein: 140-kDa surface antigen, None) , ( protein: amino acid sequence, None) , ( protein: mAb J393, None) , ( cell type: leukocytes, None) , ( protein: NH2, None) , ( protein: CD43/leukosialin, None) , ( protein: sialoglycoprotein, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: 140-kDa surface antigen, None) , ( protein: amino acid sequence, None) , ( protein: mAb J393, None) , ( cell type: leukocytes, None) , ( protein: NH2, None) , ( protein: CD43/leukosialin, None) , ( protein: sialoglycoprotein, None) " }

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{ "id": "2832", "sentence": "While Jurkat cells co-expressed two discrete cell-surface isoforms of CD43 , recognized by mAb J393 and mAb G10-2 , respectively, only J393/ CD43 signaled apoptosis .", "label": " ( protein: mAb J393, None) , ( protein: cell-surface isoforms, None) , ( protein: mAb G10-2, None) , ( cell line: Jurkat cells, None) , ( protein: CD43, None) , ( protein: CD43, None) , ( protein: J393/ CD43, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: mAb J393, None) , ( protein: cell-surface isoforms, None) , ( protein: mAb G10-2, None) , ( cell line: Jurkat cells, None) , ( protein: CD43, None) , ( protein: CD43, None) , ( protein: J393/ CD43, None) " }

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{ "id": "2833", "sentence": "J393/ CD43 was found to be hyposialylated, bearing predominantly O-linked monosaccharide glycans , whereas G10-2/ CD43 bore complex sialylated tetra- and hexasaccharide chains .", "label": " ( protein: CD43, None) , ( protein: CD43, None) , ( protein: J393/ CD43, None) , ( protein: G10-2/ CD43, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: CD43, None) , ( protein: CD43, None) , ( protein: J393/ CD43, None) , ( protein: G10-2/ CD43, None) " }

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{ "id": "2834", "sentence": "Treatment with soluble, bivalent mAb J393 killed 25-50% of the cell population , while concomitant engagement of either the CD3.TcR complex or the integrins CD18 and CD29 significantly potentiated this effect.", "label": " ( protein: CD18, None) , ( protein: mAb J393, None) , ( cell line: cell population, None) , ( protein: CD3.TcR complex, None) , ( protein: CD29, None) , ( protein: integrins, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: CD18, None) , ( protein: mAb J393, None) , ( cell line: cell population, None) , ( protein: CD3.TcR complex, None) , ( protein: CD29, None) , ( protein: integrins, None) " }

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{ "id": "2835", "sentence": "Treatment of Jurkat cells with mAb J393 induced tyrosine phosphorylation of specific protein substrates that underwent hyperphosphorylation upon antigen receptor costimulation .", "label": " ( cell line: Jurkat cells, None) , ( protein: protein substrates, None) , ( protein: mAb J393, None) , ( protein: antigen receptor, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell line: Jurkat cells, None) , ( protein: protein substrates, None) , ( protein: mAb J393, None) , ( protein: antigen receptor, None) " }

[]

{ "id": "2836", "sentence": "Tyrosine kinase inhibition by herbimycin A diminished J393/ CD43 -mediated apoptosis , whereas inhibition of phosphotyrosine phosphatase activity by bis(maltolato)oxovanadium-IV enhanced cell death.", "label": " ( protein: J393/ CD43, None) , ( protein: Tyrosine kinase, None) , ( protein: phosphotyrosine phosphatase, None) , ( protein: CD43, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: J393/ CD43, None) , ( protein: Tyrosine kinase, None) , ( protein: phosphotyrosine phosphatase, None) , ( protein: CD43, None) " }

[]

{ "id": "2837", "sentence": "Signal transduction through tyrosine kinase activation may lead to altered gene expression, as J393/ CD43 ligation prompted decreases in the nuclear localization of the transcriptional regulatory protein NF-kappaB and proteins binding the interferon-inducible regulatory element .", "label": " ( protein: NF-kappaB, None) , ( protein: transcriptional regulatory protein, None) , ( DNA: interferon-inducible regulatory element, None) , ( protein: CD43, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: NF-kappaB, None) , ( protein: transcriptional regulatory protein, None) , ( DNA: interferon-inducible regulatory element, None) , ( protein: CD43, None) " }

[]

{ "id": "2838", "sentence": "Since peripheral blood T-lymphocytes express cryptic epitopes for mAb J393 , these findings demonstrate the existence of a tightly regulated CD43 -mediated pathway for inducing apoptosis in human T-cell lineages .", "label": " ( cell type: peripheral blood T-lymphocytes, None) , ( protein: cryptic epitopes, None) , ( protein: mAb J393, None) , ( protein: CD43, None) , ( cell type: human T-cell lineages, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell type: peripheral blood T-lymphocytes, None) , ( protein: cryptic epitopes, None) , ( protein: mAb J393, None) , ( protein: CD43, None) , ( cell type: human T-cell lineages, None) " }

[]

{ "id": "2839", "sentence": "Sterol dependent LDL-receptor gene transcription in lymphocytes from normal and CML patients .", "label": " ( cell type: lymphocytes, None) , ( DNA: LDL-receptor gene, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell type: lymphocytes, None) , ( DNA: LDL-receptor gene, None) " }

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{ "id": "2840", "sentence": "Sterol regulatory element ( SRE ) has been recognized to regulate various key genes coding for especially low density lipoprotein ( LDL )-receptor , 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase and HMG-CoA synthase known to play a crucial role in the cholesterol feedback mechanism .", "label": " ( protein: low density lipoprotein, None) , ( protein: 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase, None) , ( DNA: genes, None) , ( DNA: Sterol regulatory element, None) , ( protein: HMG-CoA synthase, None) , ( protein: low density lipoprotein ( LDL )-receptor, None) , ( protein: LDL, None) , ( DNA: SRE, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: low density lipoprotein, None) , ( protein: 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase, None) , ( DNA: genes, None) , ( DNA: Sterol regulatory element, None) , ( protein: HMG-CoA synthase, None) , ( protein: low density lipoprotein ( LDL )-receptor, None) , ( protein: LDL, None) , ( DNA: SRE, None) " }

[]

{ "id": "2841", "sentence": "The deranged cholesterol feedback mechanism has been widely recognised in initiation as well as progression of various types of cancers including chronic myeloid leukaemia ( CML ).", "label": " None", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " None" }

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{ "id": "2842", "sentence": "Consequently, the present study was addressed to understand this phenomenon and revealed the existence of a unique 47 kDa protein factor having affinity for this SRE sequence in lymphocytes from normal subjects as well as its absence in lymphocytes from untreated CML patients .", "label": " ( cell type: lymphocytes, None) , ( cell type: lymphocytes, None) , ( protein: unique 47 kDa protein factor, None) , ( DNA: SRE sequence, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell type: lymphocytes, None) , ( cell type: lymphocytes, None) , ( protein: unique 47 kDa protein factor, None) , ( DNA: SRE sequence, None) " }

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{ "id": "2843", "sentence": "However, this factor appeared when the CML patients achieved complete haematological remission ( CHR ) through alpha-interferon therapy .", "label": " ( protein: alpha-interferon, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: alpha-interferon, None) " }

[]

{ "id": "2844", "sentence": "Further, an inverse relationship was also observed between sterol modulated LDL -receptor gene transcription and the binding affinity of this 47 kDa factor to the SRE sequence .", "label": " ( protein: 47 kDa factor, None) , ( DNA: SRE sequence, None) , ( protein: LDL, None) , ( DNA: LDL -receptor gene, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: 47 kDa factor, None) , ( DNA: SRE sequence, None) , ( protein: LDL, None) , ( DNA: LDL -receptor gene, None) " }

[]

{ "id": "2845", "sentence": "Based upon these results we propose that alpha-interferon through its receptor initiates phosphatidic acid dependent signalling which in turn regulates the affinity of 47 kDa sterol regulatory element binding factor as well as LDL -receptor gene transcription in lymphocytes from CML patients .", "label": " ( protein: LDL, None) , ( cell type: lymphocytes, None) , ( DNA: LDL -receptor gene, None) , ( protein: 47 kDa sterol regulatory element binding factor, None) , ( protein: alpha-interferon, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: LDL, None) , ( cell type: lymphocytes, None) , ( DNA: LDL -receptor gene, None) , ( protein: 47 kDa sterol regulatory element binding factor, None) , ( protein: alpha-interferon, None) " }

[]

{ "id": "2846", "sentence": "Multiple prolactin-responsive elements mediate G1 and S phase expression of the interferon regulatory factor-1 gene .", "label": " ( DNA: interferon regulatory factor-1 gene, None) , ( DNA: prolactin-responsive elements, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: interferon regulatory factor-1 gene, None) , ( DNA: prolactin-responsive elements, None) " }

[]

{ "id": "2847", "sentence": "The interferon regulatory factor-1 ( IRF-1 ) gene is both an immediate-early G1 phase gene and an S phase gene inducible by PRL in rat Nb2 T lymphocytes .", "label": " ( protein: IRF-1, None) , ( DNA: interferon regulatory factor-1 ( IRF-1 ) gene, None) , ( DNA: S phase gene, None) , ( protein: PRL, None) , ( cell line: rat Nb2 T lymphocytes, None) , ( DNA: immediate-early G1 phase gene, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: IRF-1, None) , ( DNA: interferon regulatory factor-1 ( IRF-1 ) gene, None) , ( DNA: S phase gene, None) , ( protein: PRL, None) , ( cell line: rat Nb2 T lymphocytes, None) , ( DNA: immediate-early G1 phase gene, None) " }

[]

{ "id": "2848", "sentence": "To understand the mechanism by which PRL regulates the biphasic expression of IRF-1 , we cloned the rat IRF-1 gene and functionally characterized the IRF-1 promoter .", "label": " ( DNA: IRF-1 promoter, None) , ( protein: IRF-1, None) , ( DNA: rat IRF-1 gene, None) , ( protein: IRF-1, None) , ( protein: IRF-1, None) , ( protein: PRL, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: IRF-1 promoter, None) , ( protein: IRF-1, None) , ( DNA: rat IRF-1 gene, None) , ( protein: IRF-1, None) , ( protein: IRF-1, None) , ( protein: PRL, None) " }

[]

{ "id": "2849", "sentence": "Upon transfection into Nb2 T cells , 1.7 kilobases (kb) of IRF-1 5'-flanking DNA linked to a chloramphenicol acetyl transferase ( CAT ) reporter gene mediated a 30-fold induction of CAT enzyme activity in response to 24 h of PRL stimulation .", "label": " ( DNA: chloramphenicol acetyl transferase ( CAT ) reporter gene, None) , ( protein: chloramphenicol acetyl transferase, None) , ( protein: IRF-1, None) , ( protein: CAT, None) , ( DNA: IRF-1 5#$%#-flanking DNA, None) , ( protein: CAT enzyme, None) , ( protein: CAT, None) , ( cell line: Nb2 T cells, None) , ( protein: PRL, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: chloramphenicol acetyl transferase ( CAT ) reporter gene, None) , ( protein: chloramphenicol acetyl transferase, None) , ( protein: IRF-1, None) , ( protein: CAT, None) , ( DNA: IRF-1 5#$%#-flanking DNA, None) , ( protein: CAT enzyme, None) , ( protein: CAT, None) , ( cell line: Nb2 T cells, None) , ( protein: PRL, None) " }

[]

{ "id": "2850", "sentence": "Deletion mutants containing 1.3 , 0.6 , and 0.2 kb 5'-flanking DNA were incrementally less transcriptionally active, although 0.2 kb still mediated a 12-fold induction by PRL .", "label": " ( protein: PRL, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: PRL, None) " }

[]

{ "id": "2851", "sentence": "The sequence between -1.7 and -0.2 kb linked to a heterologous thymidine kinase promoter failed to respond to PRL stimulation, suggesting that the activity of upstream PRL response elements may require an interaction with promoter-proximal elements .", "label": " ( protein: PRL, None) , ( DNA: heterologous thymidine kinase promoter, None) , ( DNA: upstream PRL response elements, None) , ( DNA: promoter-proximal elements, None) , ( protein: PRL, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: PRL, None) , ( DNA: heterologous thymidine kinase promoter, None) , ( DNA: upstream PRL response elements, None) , ( DNA: promoter-proximal elements, None) , ( protein: PRL, None) " }

[]

{ "id": "2852", "sentence": "By assaying CAT enzyme activity across a 24-h PRL induction time course, we were able to assign G1 vs. S phase PRL responses of the IRF-1 gene to different regions of the IRF-1 5'-flanking and promoter DNA .", "label": " ( protein: CAT, None) , ( DNA: 5#$%#-flanking, None) , ( DNA: promoter DNA, None) , ( DNA: IRF-1 gene, None) , ( protein: PRL, None) , ( protein: IRF-1, None) , ( protein: IRF-1, None) , ( protein: PRL, None) , ( protein: CAT enzyme, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: CAT, None) , ( DNA: 5#$%#-flanking, None) , ( DNA: promoter DNA, None) , ( DNA: IRF-1 gene, None) , ( protein: PRL, None) , ( protein: IRF-1, None) , ( protein: IRF-1, None) , ( protein: PRL, None) , ( protein: CAT enzyme, None) " }

[]

{ "id": "2853", "sentence": "The 0.2-kb IRF- CAT construct was induced by PRL stimulation during the G1 phase of the cell cycle .", "label": " ( DNA: IRF- CAT construct, None) , ( protein: CAT, None) , ( protein: PRL, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: IRF- CAT construct, None) , ( protein: CAT, None) , ( protein: PRL, None) " }

[]

{ "id": "2854", "sentence": "In contrast, the 1.7-kb IRF- CAT construct was inducible by PRL during both G1 and S phase of the cell cycle .", "label": " ( protein: PRL, None) , ( protein: CAT, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: PRL, None) , ( protein: CAT, None) " }

[]

{ "id": "2855", "sentence": "Hence, the PRL -induced biphasic expression of the IRF-1 gene appears to be controlled by separate PRL -responsive elements : elements in the first 0.2 kb of the IRF-1 promoter region act during early activation , and elements between 0.2 and 1.7 kb act in concert with the proximal 0.2-kb region during S phase progression .", "label": " ( protein: PRL, None) , ( protein: IRF-1, None) , ( DNA: IRF-1 gene, None) , ( DNA: PRL -responsive elements, None) , ( protein: IRF-1, None) , ( DNA: IRF-1 promoter region, None) , ( protein: PRL, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: PRL, None) , ( protein: IRF-1, None) , ( DNA: IRF-1 gene, None) , ( DNA: PRL -responsive elements, None) , ( protein: IRF-1, None) , ( DNA: IRF-1 promoter region, None) , ( protein: PRL, None) " }

[]

{ "id": "2856", "sentence": "Regulation of the BZLF1 promoter of Epstein-Barr virus by second messengers in anti-immunoglobulin-treated B cells .", "label": " ( cell line: anti-immunoglobulin-treated B cells, None) , ( DNA: BZLF1 promoter, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell line: anti-immunoglobulin-treated B cells, None) , ( DNA: BZLF1 promoter, None) " }

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{ "id": "2857", "sentence": "Initiation of the Epstein-Barr virus (EBV) lytic cycle is dependent on the transcription of the BZLF1 gene .", "label": " ( DNA: BZLF1 gene, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: BZLF1 gene, None) " }

[]

{ "id": "2858", "sentence": "The BZLF1 gene promoter ( Zp ) was activated by crosslinking of cell surface immunoglobulin ( Ig ) with anti-Ig antibody in B cells , even in the absence of other viral genes.", "label": " ( protein: cell surface immunoglobulin, None) , ( cell type: B cells, None) , ( protein: Ig, None) , ( DNA: BZLF1 gene promoter, None) , ( DNA: BZLF1 gene, None) , ( DNA: Zp, None) , ( protein: anti-Ig antibody, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: cell surface immunoglobulin, None) , ( cell type: B cells, None) , ( protein: Ig, None) , ( DNA: BZLF1 gene promoter, None) , ( DNA: BZLF1 gene, None) , ( DNA: Zp, None) , ( protein: anti-Ig antibody, None) " }

[]

{ "id": "2859", "sentence": "We identified several anti-Ig response elements within Zp , which were originally defined as 12-O-tetradecanoylphorbol-13-acetate ( TPA ) response elements ( ZI repeats and ZII , an AP-1-like domain ).", "label": " ( DNA: AP-1-like domain, None) , ( DNA: 12-O-tetradecanoylphorbol-13-acetate ( TPA ) response elements, None) , ( DNA: ZII, None) , ( DNA: Zp, None) , ( DNA: ZI repeats, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: AP-1-like domain, None) , ( DNA: 12-O-tetradecanoylphorbol-13-acetate ( TPA ) response elements, None) , ( DNA: ZII, None) , ( DNA: Zp, None) , ( DNA: ZI repeats, None) " }

[]

{ "id": "2860", "sentence": "Since anti-Ig crosslinking leads to activation of protein kinase C ( PKC ) and an increase in intracellular calcium level, Zp was tested for the response to these cellular factors .", "label": " ( protein: cellular factors, None) , ( protein: PKC, None) , ( DNA: Zp, None) , ( protein: protein kinase C, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: cellular factors, None) , ( protein: PKC, None) , ( DNA: Zp, None) , ( protein: protein kinase C, None) " }

[]

{ "id": "2861", "sentence": "Treatment with calcium ionophore A23187 increased Zp activity .", "label": " ( DNA: Zp, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: Zp, None) " }

[]

{ "id": "2862", "sentence": "When the calcium ionophore was used in conjunction with TPA , a PKC activator, the Zp induction was synergistically enhanced.", "label": " ( protein: PKC, None) , ( DNA: Zp, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: PKC, None) , ( DNA: Zp, None) " }

[]

{ "id": "2863", "sentence": "1-(5-Isoquinolinyl sulfonyl)-2-methylpiperazine , an inhibitor of PKC , inhibited the anti-Ig inducibility of Zp .", "label": " ( protein: anti-Ig, None) , ( protein: PKC, None) , ( DNA: Zp, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: anti-Ig, None) , ( protein: PKC, None) , ( DNA: Zp, None) " }

[]

{ "id": "2864", "sentence": "Calmodulin antagonists , compound R24571 and trifluoperazine , blocked the Zp activation with anti-Ig .", "label": " ( protein: anti-Ig, None) , ( DNA: Zp, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: anti-Ig, None) , ( DNA: Zp, None) " }

[]

{ "id": "2865", "sentence": "These findings suggest that Zp responds directly to changes in the activity of both PKC and calcium /calmodulin-dependent protein kinase .", "label": " ( protein: calcium /calmodulin-dependent protein kinase, None) , ( DNA: Zp, None) , ( protein: PKC, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: calcium /calmodulin-dependent protein kinase, None) , ( DNA: Zp, None) , ( protein: PKC, None) " }

[]

{ "id": "2866", "sentence": "Requirement of tyrosine kinase activation for the anti-Ig -mediated Zp activation was also demonstrated through the use of the tyrosine kinase inhibitor herbimycin .", "label": " ( protein: anti-Ig, None) , ( DNA: Zp, None) , ( protein: tyrosine kinase, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: anti-Ig, None) , ( DNA: Zp, None) , ( protein: tyrosine kinase, None) " }

[]

{ "id": "2867", "sentence": "These cellular gene regulatory molecules induced with anti-Ig may cooperatively play an important part in achieving efficient EBV activation as seen with anti-Ig treatment in B cells .", "label": " ( cell type: B cells, None) , ( protein: anti-Ig, None) , ( protein: cellular gene regulatory molecules, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell type: B cells, None) , ( protein: anti-Ig, None) , ( protein: cellular gene regulatory molecules, None) " }

[]

{ "id": "2868", "sentence": "The SCL protein displays cell-specific heterogeneity in size.", "label": " ( protein: SCL protein, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: SCL protein, None) " }

[]

{ "id": "2869", "sentence": "SCL protein production was examined in a variety of hemopoietic cell lines by immunoblotting using specific polyclonal antisera .", "label": " ( protein: SCL protein, None) , ( cell line: hemopoietic cell lines, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: SCL protein, None) , ( cell line: hemopoietic cell lines, None) " }

[]

{ "id": "2870", "sentence": "SCL protein was detected in erythroid , megakaryocyte , mast and early myeloid cell lines , as well as in several lymphoid leukemia cell lines which are known to harbor SCL gene rearrangements .", "label": " ( cell line: lymphoid leukemia cell lines, None) , ( protein: SCL protein, None) , ( DNA: SCL gene rearrangements, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell line: lymphoid leukemia cell lines, None) , ( protein: SCL protein, None) , ( DNA: SCL gene rearrangements, None) " }

[]

{ "id": "2871", "sentence": "In most cell lines, proteins of molecular weight 49 and 44 kDa were found, however two myeloid cell lines expressed only lower molecular weight species of 24 and 22 kDa .", "label": " ( protein: molecular weight species of 24 and 22 kDa, None) , ( protein: molecular weight 49 and 44 kDa, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: molecular weight species of 24 and 22 kDa, None) , ( protein: molecular weight 49 and 44 kDa, None) " }

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{ "id": "2872", "sentence": "This size discrepancy appeared to be due to cell-specific translational regulation , since overexpression of a retrovirally transfected SCL gene yielded the higher molecular weight forms in most cell lines ( GP+E-86 , AT2.5 , M1 ) but only the 22 kDa form in the myeloid cell line , WEHI-3B/D+ .", "label": " ( cell line: WEHI-3B/D+, None) , ( DNA: SCL gene, None) , ( cell line: GP+E-86, None) , ( cell line: cell lines, None) , ( cell line: myeloid cell line, None) , ( cell line: M1, None) , ( cell line: AT2.5, None) , ( protein: 22 kDa form, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell line: WEHI-3B/D+, None) , ( DNA: SCL gene, None) , ( cell line: GP+E-86, None) , ( cell line: cell lines, None) , ( cell line: myeloid cell line, None) , ( cell line: M1, None) , ( cell line: AT2.5, None) , ( protein: 22 kDa form, None) " }

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{ "id": "2873", "sentence": "Overexpression of full-length SCL protein in the lymphoid cell lines , SupT1 and Raji , did not alter cell phenotype and there was no evidence for autoregulation of SCL transcription .", "label": " ( protein: SCL, None) , ( cell line: lymphoid cell lines, None) , ( protein: SCL protein, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: SCL, None) , ( cell line: lymphoid cell lines, None) , ( protein: SCL protein, None) " }

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{ "id": "2874", "sentence": "The restricted pattern of SCL protein synthesis is consistent with the restricted expression of SCL mRNA documented previously.", "label": " ( RNA: SCL mRNA, None) , ( protein: SCL protein, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( RNA: SCL mRNA, None) , ( protein: SCL protein, None) " }

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{ "id": "2875", "sentence": "In addition, the present results indicate that SCL protein size was determined by regulation of translation in a cell-specific manner .", "label": " ( protein: SCL protein, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: SCL protein, None) " }

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{ "id": "2876", "sentence": "Description and functional implications of a novel mutation in the sex-determining gene SRY .", "label": " ( DNA: sex-determining gene, None) , ( DNA: SRY, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: sex-determining gene, None) , ( DNA: SRY, None) " }

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{ "id": "2877", "sentence": "The sex-determining gene SRY was screened for molecular alteration in an XY sex-reversed female by single-strand conformation polymorphism (SSCP) technique .", "label": " ( DNA: SRY, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: SRY, None) " }

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{ "id": "2878", "sentence": "An A-to-G transition was detected which leads to an exchange of a tyrosine by a cysteine in the SRY protein .", "label": " ( DNA: SRY, None) , ( protein: SRY protein, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: SRY, None) , ( protein: SRY protein, None) " }

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{ "id": "2879", "sentence": "The affected tyrosine residue located at the C terminus of the DNA binding protein is evolutionarily strongly conserved among the members of the HMG box containing proteins .", "label": " ( protein: HMG box containing proteins, None) , ( protein: C terminus, None) , ( protein: DNA binding protein, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: HMG box containing proteins, None) , ( protein: C terminus, None) , ( protein: DNA binding protein, None) " }

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{ "id": "2880", "sentence": "Using gel shift assay and peptide synthesis such a mutation is shown to abolish the SRY protein DNA binding ability .", "label": " ( DNA: SRY, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: SRY, None) " }

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{ "id": "2881", "sentence": "The involvement of this particular amino acid in the binding specificity is also discussed.", "label": " None", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " None" }

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{ "id": "2882", "sentence": "Induction of tyrosine phosphorylation and T-cell activation by vanadate peroxide , an inhibitor of protein tyrosine phosphatases .", "label": " ( cell type: T-cell, None) , ( protein: protein tyrosine phosphatases, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell type: T-cell, None) , ( protein: protein tyrosine phosphatases, None) " }

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{ "id": "2883", "sentence": "Rapid tyrosine phosphorylation of key cellular proteins is a crucial event in the transduction of activation signals to T-lymphocytes .", "label": " ( cell type: T-lymphocytes, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell type: T-lymphocytes, None) " }

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{ "id": "2884", "sentence": "The regulatory role of protein tyrosine phosphatases ( PTPases ) in this process was explored by studying the effects of a powerful PTPase inhibitor , vanadate peroxide ( pervanadate ), on the activation cascade of Jurkat human leukaemic T-cells .", "label": " ( protein: PTPase, None) , ( protein: protein tyrosine phosphatases, None) , ( protein: PTPases, None) , ( cell line: Jurkat human leukaemic T-cells, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: PTPase, None) , ( protein: protein tyrosine phosphatases, None) , ( protein: PTPases, None) , ( cell line: Jurkat human leukaemic T-cells, None) " }

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{ "id": "2885", "sentence": "Pervanadate induced activation of the tyrosine kinases lck and fyn (4- and 3-fold respectively) and a dramatic increase in tyrosine phosphorylation of cellular proteins , notably phospholipase C gamma 1 .", "label": " ( protein: lck, None) , ( protein: cellular proteins, None) , ( protein: phospholipase C gamma 1, None) , ( protein: fyn, None) , ( protein: tyrosine kinases, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: lck, None) , ( protein: cellular proteins, None) , ( protein: phospholipase C gamma 1, None) , ( protein: fyn, None) , ( protein: tyrosine kinases, None) " }

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{ "id": "2886", "sentence": "After this event, we observed a rise in intracellular Ca2+ concentration , corresponding to an influx.", "label": " None", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " None" }

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{ "id": "2887", "sentence": "This effect required surface expression of the CD45 PTPase and was not observed in CD45-deficient variants of Jurkat cells .", "label": " ( cell line: CD45-deficient variants, None) , ( protein: CD45 PTPase, None) , ( cell line: Jurkat cells, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell line: CD45-deficient variants, None) , ( protein: CD45 PTPase, None) , ( cell line: Jurkat cells, None) " }

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{ "id": "2888", "sentence": "In the CD45-negative variant , the effect of pervanadate on tyrosine phosphorylation was globally decreased and some phosphorylated substrates were specifically missing.", "label": " ( cell line: CD45-negative variant, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell line: CD45-negative variant, None) " }

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{ "id": "2889", "sentence": "Pervanadate also stimulated transcription of the c-fos gene and accumulation of its mRNA as well as several other hallmarks of T-lymphocyte activation such as surface expression of the CD69 antigen and the interleukin 2 receptor alpha-chain (CD25).", "label": " ( RNA: mRNA, None) , ( cell type: T-lymphocyte, None) , ( DNA: c-fos gene, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( RNA: mRNA, None) , ( cell type: T-lymphocyte, None) , ( DNA: c-fos gene, None) " }

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{ "id": "2890", "sentence": "Pervanadate synergized with signals delivered by T-cell antigen receptor engagement or by a phorbol ester to induce interleukin 2 production.", "label": " ( protein: antigen receptor, None) , ( cell type: T-cell, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: antigen receptor, None) , ( cell type: T-cell, None) " }

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{ "id": "2891", "sentence": "Pervanadate activated NF-kappa B , as shown by an increase in DNA-binding activity of this transcription factor .", "label": " ( protein: NF-kappa B, None) , ( protein: transcription factor, None) , ( protein: Pervanadate activated NF-kappa B, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: NF-kappa B, None) , ( protein: transcription factor, None) , ( protein: Pervanadate activated NF-kappa B, None) " }

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{ "id": "2892", "sentence": "We thus conclude that PTPases play a crucial role in the negative regulation of signal transduction culminating in T-lymphocyte activation .", "label": " ( cell type: T-lymphocyte, None) , ( protein: PTPases, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell type: T-lymphocyte, None) , ( protein: PTPases, None) " }

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{ "id": "2893", "sentence": "Moreover, induction of tyrosine phosphorylation appears sufficient per se to initiate a complete activation programme.", "label": " None", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " None" }

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{ "id": "2894", "sentence": "Activation of nuclear factor-kappaB via T cell receptor requires a Raf kinase and Ca2+ influx.", "label": " ( protein: Raf kinase, None) , ( protein: nuclear factor-kappaB, None) , ( protein: T cell receptor, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: Raf kinase, None) , ( protein: nuclear factor-kappaB, None) , ( protein: T cell receptor, None) " }

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{ "id": "2895", "sentence": "Functional synergy between Raf and calcineurin .", "label": " ( protein: calcineurin, None) , ( protein: Raf, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: calcineurin, None) , ( protein: Raf, None) " }

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{ "id": "2896", "sentence": "Signals transduced via the TCR activate the transcription factor nuclear factor-kappaB ( NF-kappaB ), which, in turn, is critical to the transcriptional induction of many genes important for the proliferation and expression of a differentiated phenotype .", "label": " ( protein: nuclear factor-kappaB, None) , ( protein: TCR, None) , ( protein: transcription factor, None) , ( protein: NF-kappaB, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: nuclear factor-kappaB, None) , ( protein: TCR, None) , ( protein: transcription factor, None) , ( protein: NF-kappaB, None) " }

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{ "id": "2897", "sentence": "Treatment of T cells with the protein kinase C activator PMA in combination with Ca2+ ionophores mimics this process, and the two agents are often substituted for TCR stimulation , bypassing the TCR .", "label": " ( protein: protein kinase C, None) , ( cell type: T cells, None) , ( protein: TCR, None) , ( protein: TCR, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: protein kinase C, None) , ( cell type: T cells, None) , ( protein: TCR, None) , ( protein: TCR, None) " }

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{ "id": "2898", "sentence": "Here we identify intracellular signaling components involved in activation of NF-kappaB following TCR stimulation .", "label": " ( protein: NF-kappaB, None) , ( protein: TCR, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: NF-kappaB, None) , ( protein: TCR, None) " }

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{ "id": "2899", "sentence": "TCR signaling was triggered by treating Jurkat T cells with PHA or anti-CD3 Abs , and NF-kappaB activation was monitored by electrophoretic mobility shift assays and/or by kappaB -dependent reporter assays .", "label": " ( protein: kappaB, None) , ( protein: PHA, None) , ( protein: NF-kappaB, None) , ( protein: anti-CD3 Abs, None) , ( protein: TCR, None) , ( cell line: Jurkat T cells, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: kappaB, None) , ( protein: PHA, None) , ( protein: NF-kappaB, None) , ( protein: anti-CD3 Abs, None) , ( protein: TCR, None) , ( cell line: Jurkat T cells, None) " }