Datasets:

huy-nh-2000

/

instructUIE

like 0

[]

{ "id": "500", "sentence": "We, therefore, compared the activation state of the IL-2 gene promoter region in freshly isolated and stimulated Ipr CD4-8- T cells with that of normal T lymphocytes .", "label": " ( DNA: IL-2 gene promoter region, None) , ( protein: IL-2, None) , ( cell line: Ipr CD4-8- T cells, None) , ( cell type: normal T lymphocytes, None) , ( cell type: T cells, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: IL-2 gene promoter region, None) , ( protein: IL-2, None) , ( cell line: Ipr CD4-8- T cells, None) , ( cell type: normal T lymphocytes, None) , ( cell type: T cells, None) " }

[]

{ "id": "501", "sentence": "Levels of the octamer , NF-kappa B ( p50-p65 heterodimer ), and AP-1 transcriptional factors are constitutively elevated in freshly isolated Ipr CD4-8- T cells , consistent with the activated phenotype of these cells.", "label": " ( protein: p50-p65 heterodimer, None) , ( protein: AP-1 transcriptional factors, None) , ( cell line: Ipr CD4-8- T cells, None) , ( protein: NF-kappa B, None) , ( cell type: T cells, None) , ( protein: AP-1, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: p50-p65 heterodimer, None) , ( protein: AP-1 transcriptional factors, None) , ( cell line: Ipr CD4-8- T cells, None) , ( protein: NF-kappa B, None) , ( cell type: T cells, None) , ( protein: AP-1, None) " }

[]

{ "id": "502", "sentence": "Upon stimulation with mitogens , formation of the transactivating complex, nuclear factor of activated T cells ( NF-AT ), occurs with normal kinetics in Ipr CD4-8- T cells .", "label": " ( cell type: T cells, None) , ( cell line: Ipr CD4-8- T cells, None) , ( protein: NF-AT, None) , ( cell type: T cells, None) , ( protein: nuclear factor of activated T cells, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell type: T cells, None) , ( cell line: Ipr CD4-8- T cells, None) , ( protein: NF-AT, None) , ( cell type: T cells, None) , ( protein: nuclear factor of activated T cells, None) " }

[]

{ "id": "503", "sentence": "Yet, the levels of the activating NF-AT complex never reach those observed in similarly stimulated normal T cells .", "label": " ( cell type: T cells, None) , ( protein: NF-AT complex, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell type: T cells, None) , ( protein: NF-AT complex, None) " }

[]

{ "id": "504", "sentence": "Furthermore, nuclear extracts from Ipr CD4-8- T cells display high levels of a novel specific binding activity at the NF-AT site , which is present at much lower levels in freshly isolated normal T lymphocytes .", "label": " ( cell type: T cells, None) , ( DNA: NF-AT site, None) , ( protein: NF-AT, None) , ( cell line: Ipr CD4-8- T cells, None) , ( cell type: normal T lymphocytes, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell type: T cells, None) , ( DNA: NF-AT site, None) , ( protein: NF-AT, None) , ( cell line: Ipr CD4-8- T cells, None) , ( cell type: normal T lymphocytes, None) " }

[]

{ "id": "505", "sentence": "Upon mitogenic stimulation , the binding activity of the novel NF-AT -binding factor is rapidly down-regulated in normal T cells , but persists at high levels in Ipr CD4-8- T cells .", "label": " ( protein: NF-AT -binding factor, None) , ( cell line: Ipr CD4-8- T cells, None) , ( protein: NF-AT, None) , ( cell type: T cells, None) , ( cell type: T cells, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: NF-AT -binding factor, None) , ( cell line: Ipr CD4-8- T cells, None) , ( protein: NF-AT, None) , ( cell type: T cells, None) , ( cell type: T cells, None) " }

[]

{ "id": "506", "sentence": "These two abnormalities at the NF-AT site provide a potential mechanism to account for the defect in IL-2 production from Ipr CD4-8- T cells .", "label": " ( cell type: T cells, None) , ( DNA: NF-AT site, None) , ( cell line: Ipr CD4-8- T cells, None) , ( protein: IL-2, None) , ( protein: NF-AT, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell type: T cells, None) , ( DNA: NF-AT site, None) , ( cell line: Ipr CD4-8- T cells, None) , ( protein: IL-2, None) , ( protein: NF-AT, None) " }

[]

{ "id": "507", "sentence": "Cross-linking of CD30 induces HIV expression in chronically infected T cells .", "label": " ( cell type: T cells, None) , ( protein: CD30, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell type: T cells, None) , ( protein: CD30, None) " }

[]

{ "id": "508", "sentence": "CD30 , a member of the tumor necrosis factor (TNF) receptor family, is expressed constitutively on the surface of the human T cell line ACH-2, which is chronically infected with human immunodeficiency virus type-1 ( HIV )-1.", "label": " ( protein: CD30, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: CD30, None) " }

[]

{ "id": "509", "sentence": "We demonstrate that cross-linking CD30 with an anti- CD30 -specific monoclonal antibody, which mimics the described biological activities of the CD30 ligand ( CD30L ), results in HIV expression .", "label": " ( protein: CD30, None) , ( protein: CD30 ligand, None) , ( protein: CD30, None) , ( protein: CD30L, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: CD30, None) , ( protein: CD30 ligand, None) , ( protein: CD30, None) , ( protein: CD30L, None) " }

[]

{ "id": "510", "sentence": "CD30 cross-linking does not alter proliferation of ACH-2 cells and the induction of HIV expression is not mediated by endogenous TNF alpha/beta .", "label": " ( cell line: ACH-2 cells, None) , ( protein: TNF alpha/beta, None) , ( protein: CD30, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell line: ACH-2 cells, None) , ( protein: TNF alpha/beta, None) , ( protein: CD30, None) " }

[]

{ "id": "511", "sentence": "Furthermore, cross-linking of CD30 leads to NF-kappa B activation and enhanced HIV transcription .", "label": " ( protein: CD30, None) , ( protein: NF-kappa B, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: CD30, None) , ( protein: NF-kappa B, None) " }

[]

{ "id": "512", "sentence": "Thus, CD30 -CD30L interactions mediate the induction of HIV expression by a kappa B-dependent pathway that is independent of TNF .", "label": " ( protein: TNF, None) , ( protein: -CD30L, None) , ( protein: CD30, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: TNF, None) , ( protein: -CD30L, None) , ( protein: CD30, None) " }

[]

{ "id": "513", "sentence": "This mechanism may be important in the activation of HIV expression from latently infected CD4+ T cells , especially in lymphoid organs where cell to cell contact is conducive to receptor-ligand interactions .", "label": " ( cell type: T cells, None) , ( cell line: CD4+ T cells, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell type: T cells, None) , ( cell line: CD4+ T cells, None) " }

[]

{ "id": "514", "sentence": "Human MHC class II gene transcription directed by the carboxyl terminus of CIITA , one of the defective genes in type II MHC combined immune deficiency .", "label": " ( DNA: CIITA, None) , ( DNA: MHC class II gene, None) , ( protein: carboxyl terminus, None) , ( DNA: defective genes, None) , ( protein: MHC, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: CIITA, None) , ( DNA: MHC class II gene, None) , ( protein: carboxyl terminus, None) , ( DNA: defective genes, None) , ( protein: MHC, None) " }

[]

{ "id": "515", "sentence": "Type II major histocompatibility complex combined immune deficiency ( type II MHC CID or bare lymphocyte syndrome ) is a congenital immunodeficiency disease characterized by absent MHC class II expression .", "label": " ( protein: MHC, None) , ( protein: MHC class II, None) , ( protein: Type II major histocompatibility complex, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: MHC, None) , ( protein: MHC class II, None) , ( protein: Type II major histocompatibility complex, None) " }

[]

{ "id": "516", "sentence": "Four distinct complementation groups have been identified.", "label": " None", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " None" }

[]

{ "id": "517", "sentence": "Recently, the defective gene in group II type II MHC CID has been isolated and termed CIITA .", "label": " ( protein: MHC, None) , ( DNA: defective gene, None) , ( DNA: CIITA, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: MHC, None) , ( DNA: defective gene, None) , ( DNA: CIITA, None) " }

[]

{ "id": "518", "sentence": "Here, we demonstrate that CIITA is an MHC class II gene-specific transcription activator .", "label": " ( protein: MHC class II, None) , ( DNA: CIITA, None) , ( DNA: MHC class II gene-specific transcription activator, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: MHC class II, None) , ( DNA: CIITA, None) , ( DNA: MHC class II gene-specific transcription activator, None) " }

[]

{ "id": "519", "sentence": "The transcription activation function is provided by the N-terminal acidic domain ( amino acids 26-137 ), which is experimentally exchangeable with a heterologous viral transcription-activating domain .", "label": " ( protein: heterologous viral transcription-activating domain, None) , ( protein: N-terminal acidic domain, None) , ( protein: amino acids 26-137, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: heterologous viral transcription-activating domain, None) , ( protein: N-terminal acidic domain, None) , ( protein: amino acids 26-137, None) " }

[]

{ "id": "520", "sentence": "The specificity of CIITA for three major MHC class II genes , DR , DQ and DP , is mediated by its remaining C-terminal residues ( amino acids 317-1130 ).", "label": " ( DNA: MHC class II genes, None) , ( protein: CIITA, None) , ( protein: amino acids 317-1130, None) , ( protein: C-terminal residues, None) , ( protein: MHC class II, None) , ( DNA: DP, None) , ( DNA: DQ, None) , ( DNA: DR, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: MHC class II genes, None) , ( protein: CIITA, None) , ( protein: amino acids 317-1130, None) , ( protein: C-terminal residues, None) , ( protein: MHC class II, None) , ( DNA: DP, None) , ( DNA: DQ, None) , ( DNA: DR, None) " }

[]

{ "id": "521", "sentence": "The transactivation of multiple cis elements , especially S and X2 , of the DR alpha proximal promoter in group II CID cells is CIITA dependent.", "label": " ( DNA: X2, None) , ( DNA: proximal promoter, None) , ( DNA: multiple cis elements, None) , ( cell line: group II CID cells, None) , ( protein: CIITA, None) , ( DNA: DR alpha proximal promoter, None) , ( DNA: S, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: X2, None) , ( DNA: proximal promoter, None) , ( DNA: multiple cis elements, None) , ( cell line: group II CID cells, None) , ( protein: CIITA, None) , ( DNA: DR alpha proximal promoter, None) , ( DNA: S, None) " }

[]

{ "id": "522", "sentence": "Since CIITA overexpression in normal cells did not increase class II expression , we propose that initiation of CIITA expression serves as the on-off switch, while availability of downstream interactor (s) limits transcription .", "label": " ( protein: CIITA, None) , ( protein: CIITA, None) , ( cell type: normal cells, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: CIITA, None) , ( protein: CIITA, None) , ( cell type: normal cells, None) " }

[]

{ "id": "523", "sentence": "Monocyte tethering by P-selectin regulates monocyte chemotactic protein-1 and tumor necrosis factor-alpha secretion .", "label": " ( protein: P-selectin, None) , ( protein: tumor necrosis factor-alpha, None) , ( protein: monocyte chemotactic protein-1, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: P-selectin, None) , ( protein: tumor necrosis factor-alpha, None) , ( protein: monocyte chemotactic protein-1, None) " }

[]

{ "id": "524", "sentence": "Signal integration and NF-kappa B translocation [see comments]", "label": " ( protein: NF-kappa B, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: NF-kappa B, None) " }

[]

{ "id": "525", "sentence": "Adhesion molecules that tether circulating leukocytes to endothelial cells may also transduce or modulate outside-in signals for cellular activation , providing an initial regulatory point in the inflammatory response .", "label": " ( protein: Adhesion molecules, None) , ( cell type: endothelial cells, None) , ( cell type: circulating leukocytes, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: Adhesion molecules, None) , ( cell type: endothelial cells, None) , ( cell type: circulating leukocytes, None) " }

[]

{ "id": "526", "sentence": "Adhesion of human monocytes to P-selectin , the most rapidly expressed endothelial tethering factor , increased the secretion of monocyte chemotactic protein-1 ( MCP-1 ) and tumor necrosis factor-alpha ( TNF-alpha ) by the leukocytes when they were stimulated with platelet-activating factor .", "label": " ( protein: MCP-1, None) , ( protein: platelet-activating factor, None) , ( protein: P-selectin, None) , ( cell type: human monocytes, None) , ( protein: TNF-alpha, None) , ( cell type: leukocytes, None) , ( protein: monocyte chemotactic protein-1, None) , ( protein: tumor necrosis factor-alpha, None) , ( cell type: monocytes, None) , ( protein: endothelial tethering factor, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: MCP-1, None) , ( protein: platelet-activating factor, None) , ( protein: P-selectin, None) , ( cell type: human monocytes, None) , ( protein: TNF-alpha, None) , ( cell type: leukocytes, None) , ( protein: monocyte chemotactic protein-1, None) , ( protein: tumor necrosis factor-alpha, None) , ( cell type: monocytes, None) , ( protein: endothelial tethering factor, None) " }

[]

{ "id": "527", "sentence": "Increased cytokine secretion was specifically inhibited by G1 , an anti- P-selectin mAb that prevents P-selectin from binding to its ligand ( P-selectin glycoprotein ligand-1 ) on myeloid cells .", "label": " ( protein: anti- P-selectin mAb, None) , ( protein: P-selectin, None) , ( cell type: myeloid cells, None) , ( protein: P-selectin glycoprotein ligand-1, None) , ( protein: G1, None) , ( protein: P-selectin, None) , ( protein: P-selectin, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: anti- P-selectin mAb, None) , ( protein: P-selectin, None) , ( cell type: myeloid cells, None) , ( protein: P-selectin glycoprotein ligand-1, None) , ( protein: G1, None) , ( protein: P-selectin, None) , ( protein: P-selectin, None) " }

[]

{ "id": "528", "sentence": "Moreover, tethering by P-selectin specifically enhanced nuclear translocation of nuclear factor-kappa B ( NF-kappa B ), a transcription factor required for expression of MCP-1 , TNF-alpha , and other immediate-early genes .", "label": " ( protein: MCP-1, None) , ( protein: P-selectin, None) , ( protein: TNF-alpha, None) , ( protein: nuclear factor-kappa B, None) , ( protein: NF-kappa B, None) , ( protein: transcription factor, None) , ( DNA: immediate-early genes, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: MCP-1, None) , ( protein: P-selectin, None) , ( protein: TNF-alpha, None) , ( protein: nuclear factor-kappa B, None) , ( protein: NF-kappa B, None) , ( protein: transcription factor, None) , ( DNA: immediate-early genes, None) " }

[]

{ "id": "529", "sentence": "These results demonstrate that P-selectin , through its ligands on monocytes , may locally regulate cytokine secretion in inflamed tissues .", "label": " ( protein: cytokine, None) , ( cell type: monocytes, None) , ( protein: P-selectin, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: cytokine, None) , ( cell type: monocytes, None) , ( protein: P-selectin, None) " }

[]

{ "id": "530", "sentence": "Functional roles of in vivo footprinted DNA motifs within an alpha-globin enhancer .", "label": " ( DNA: in vivo footprinted DNA motifs, None) , ( DNA: alpha-globin enhancer, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: in vivo footprinted DNA motifs, None) , ( DNA: alpha-globin enhancer, None) " }

[]

{ "id": "531", "sentence": "Erythroid lineage and developmental stage specificities.", "label": " None", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " None" }

[]

{ "id": "532", "sentence": "Transcriptional regulation of the human alpha-like globin genes , embryonic zeta 2 and adult alpha , during erythroid development is mediated by a distal enhancer , HS-40 .", "label": " ( DNA: distal enhancer, None) , ( DNA: HS-40, None) , ( DNA: human alpha-like globin genes, None) , ( DNA: adult alpha, None) , ( DNA: embryonic zeta 2, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: distal enhancer, None) , ( DNA: HS-40, None) , ( DNA: human alpha-like globin genes, None) , ( DNA: adult alpha, None) , ( DNA: embryonic zeta 2, None) " }

[]

{ "id": "533", "sentence": "Previous protein-DNA binding studies have shown that HS-40 consists of multiple nuclear factor binding motifs that are occupied in vivo in an erythroid lineage- and developmental stage- specific manner .", "label": " ( DNA: HS-40, None) , ( DNA: nuclear factor binding motifs, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: HS-40, None) , ( DNA: nuclear factor binding motifs, None) " }

[]

{ "id": "534", "sentence": "We have systematically analyzed the functional roles of these factor binding motifs of HS-40 by site-directed mutagenesis and transient expression assay in erythroid cell cultures .", "label": " ( cell line: erythroid cell cultures, None) , ( DNA: factor binding motifs, None) , ( DNA: HS-40, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell line: erythroid cell cultures, None) , ( DNA: factor binding motifs, None) , ( DNA: HS-40, None) " }

[]

{ "id": "535", "sentence": "Three of these HS-40 enhancer motifs , 5'NF-E2/AP1 , GT II , and GATA-1(c) , positively regulate the zeta 2-globin promoter activity in embryonic/fetal erythroid K562 cells and the adult alpha -globin promoter activity in adult erythroid MEL cells .", "label": " ( DNA: adult alpha -globin promoter, None) , ( DNA: GATA-1(c), None) , ( cell line: embryonic/fetal erythroid K562 cells, None) , ( DNA: HS-40 enhancer motifs, None) , ( DNA: adult alpha, None) , ( cell line: adult erythroid MEL cells, None) , ( DNA: zeta 2-globin promoter, None) , ( DNA: GT II, None) , ( DNA: HS-40, None) , ( DNA: 5#$%#NF-E2/AP1, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: adult alpha -globin promoter, None) , ( DNA: GATA-1(c), None) , ( cell line: embryonic/fetal erythroid K562 cells, None) , ( DNA: HS-40 enhancer motifs, None) , ( DNA: adult alpha, None) , ( cell line: adult erythroid MEL cells, None) , ( DNA: zeta 2-globin promoter, None) , ( DNA: GT II, None) , ( DNA: HS-40, None) , ( DNA: 5#$%#NF-E2/AP1, None) " }

[]

{ "id": "536", "sentence": "On the other hand, the 3'NF-E2/AP1 motif is able to exert both positive and negative regulatory effects on the zeta 2-globin promoter activity in K562 cells , and this dual function appears to be modulated through differential binding of the ubiquitous AP1 factors and the erythroid-enriched NF-E2 factor .", "label": " ( DNA: 3#$%#NF-E2/AP1 motif, None) , ( cell line: K562 cells, None) , ( DNA: zeta 2-globin promoter, None) , ( protein: ubiquitous AP1 factors, None) , ( protein: erythroid-enriched NF-E2 factor, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: 3#$%#NF-E2/AP1 motif, None) , ( cell line: K562 cells, None) , ( DNA: zeta 2-globin promoter, None) , ( protein: ubiquitous AP1 factors, None) , ( protein: erythroid-enriched NF-E2 factor, None) " }

[]

{ "id": "537", "sentence": "Mutation in the GATA-1(d) motif , which exhibits an adult erythroid-specific genomic footprint , decreases the HS-40 enhancer function in dimethyl sulfoxide-induced MEL cells but not in K562 cells .", "label": " ( DNA: HS-40, None) , ( DNA: HS-40 enhancer, None) , ( cell line: dimethyl sulfoxide-induced MEL cells, None) , ( cell line: K562 cells, None) , ( DNA: GATA-1(d) motif, None) , ( DNA: adult erythroid-specific genomic footprint, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: HS-40, None) , ( DNA: HS-40 enhancer, None) , ( cell line: dimethyl sulfoxide-induced MEL cells, None) , ( cell line: K562 cells, None) , ( DNA: GATA-1(d) motif, None) , ( DNA: adult erythroid-specific genomic footprint, None) " }

[]

{ "id": "538", "sentence": "These studies have defined the regulatory roles of the different HS-40 motifs .", "label": " ( DNA: HS-40 motifs, None) , ( DNA: HS-40, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: HS-40 motifs, None) , ( DNA: HS-40, None) " }

[]

{ "id": "539", "sentence": "The remarkable correlation between genomic footprinting data and the mutagenesis results also suggests that the erythroid lineage- and developmental stage- specific regulation of human alpha-like globin promoters is indeed modulated by stable binding of specific nuclear factors in vivo.", "label": " ( DNA: human alpha-like globin promoters, None) , ( protein: specific nuclear factors, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: human alpha-like globin promoters, None) , ( protein: specific nuclear factors, None) " }

[]

{ "id": "540", "sentence": "Nitric oxide -stimulated guanine nucleotide exchange on p21ras .", "label": " ( protein: p21ras, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: p21ras, None) " }

[]

{ "id": "541", "sentence": "The protooncogene p21ras , a monomeric G protein family member , plays a critical role in converting extracellular signals into intracellular biochemical events .", "label": " ( DNA: protooncogene p21ras, None) , ( protein: monomeric G protein family member, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: protooncogene p21ras, None) , ( protein: monomeric G protein family member, None) " }

[]

{ "id": "542", "sentence": "Here, we report that nitric oxide ( NO ) activates p21ras in human T cells as evidenced by an increase in GTP -bound p21ras .", "label": " ( cell type: human T cells, None) , ( DNA: p21ras, None) , ( cell type: T cells, None) , ( protein: GTP -bound p21ras, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell type: human T cells, None) , ( DNA: p21ras, None) , ( cell type: T cells, None) , ( protein: GTP -bound p21ras, None) " }

[]

{ "id": "543", "sentence": "In vitro studies using pure recombinant p21ras demonstrate that the activation is direct and reversible.", "label": " ( protein: pure recombinant p21ras, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: pure recombinant p21ras, None) " }

[]

{ "id": "544", "sentence": "Circular dichroism analysis reveals that NO induces a profound conformational change in p21ras in association with GDP /GTP exchange .", "label": " ( DNA: p21ras, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: p21ras, None) " }

[]

{ "id": "545", "sentence": "The mechanism of activation is due to S-nitrosylation of a critical cysteine residue which stimulates guanine nucleotide exchange.", "label": " None", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " None" }

[]

{ "id": "546", "sentence": "Furthermore, we demonstrate that p21ras is essential for NO -induced downstream signaling , such as NF-kappa B activation , and that endogenous NO can activate p21ras in the same cell.", "label": " ( protein: p21ras, None) , ( protein: p21ras, None) , ( protein: NF-kappa B, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: p21ras, None) , ( protein: p21ras, None) , ( protein: NF-kappa B, None) " }

[]

{ "id": "547", "sentence": "These studies identify p21ras as a target of the same cell.", "label": " ( protein: p21ras, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: p21ras, None) " }

[]

{ "id": "548", "sentence": "These studies identify p21ras as a target of NO in T cells and suggest that NO activates p21ras by an action which mimics that of guanine nucleotide exchange factors .", "label": " ( cell type: T cells, None) , ( protein: p21ras, None) , ( protein: guanine nucleotide exchange factors, None) , ( protein: p21ras, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell type: T cells, None) , ( protein: p21ras, None) , ( protein: guanine nucleotide exchange factors, None) , ( protein: p21ras, None) " }

[]

{ "id": "549", "sentence": "Interleukin-2 promoter activity in Epstein-Barr virus -transformed B lymphocytes is controlled by nuclear factor-chi B .", "label": " ( cell line: Epstein-Barr virus -transformed B lymphocytes, None) , ( protein: nuclear factor-chi B, None) , ( DNA: Interleukin-2 promoter, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell line: Epstein-Barr virus -transformed B lymphocytes, None) , ( protein: nuclear factor-chi B, None) , ( DNA: Interleukin-2 promoter, None) " }

[]

{ "id": "550", "sentence": "The regulation of interleukin (IL)-2 gene expression has been investigated mainly in T lymphocytes , the predominant producers of IL-2 .", "label": " ( cell type: T lymphocytes, None) , ( DNA: interleukin (IL)-2 gene, None) , ( protein: IL-2, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell type: T lymphocytes, None) , ( DNA: interleukin (IL)-2 gene, None) , ( protein: IL-2, None) " }

[]

{ "id": "551", "sentence": "However, B cells can also synthesize IL-2 .", "label": " ( protein: IL-2, None) , ( cell type: B cells, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: IL-2, None) , ( cell type: B cells, None) " }

[]

{ "id": "552", "sentence": "In the present study we analyzed the control of IL-2 promoter activity in Epstein-Barr virus ( EBV )-transformed B cell clones which are capable of secreting IL-2 at a low level after stimulation with phorbol 12-myristate 13-acetate and the Ca2+ ionophore ionomycin .", "label": " ( protein: IL-2, None) , ( DNA: IL-2 promoter, None) , ( cell line: Epstein-Barr virus ( EBV )-transformed B cell clones, None) , ( protein: IL-2, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: IL-2, None) , ( DNA: IL-2 promoter, None) , ( cell line: Epstein-Barr virus ( EBV )-transformed B cell clones, None) , ( protein: IL-2, None) " }

[]

{ "id": "553", "sentence": "Transient transfections using reporter constructs with multiples of transcription factor binding sites from the IL-2 promoter [ distal nuclear factor (NF)-AT , proximal NF-AT , AP-1/Octamer ( UPS ) or NF-chi B ( TCEd ) sites] were performed.", "label": " ( protein: NF-chi B, None) , ( protein: UPS, None) , ( protein: proximal NF-AT, None) , ( DNA: reporter constructs, None) , ( protein: AP-1/Octamer, None) , ( protein: IL-2, None) , ( protein: TCEd, None) , ( DNA: IL-2 promoter, None) , ( DNA: transcription factor binding sites, None) , ( protein: distal nuclear factor (NF)-AT, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: NF-chi B, None) , ( protein: UPS, None) , ( protein: proximal NF-AT, None) , ( DNA: reporter constructs, None) , ( protein: AP-1/Octamer, None) , ( protein: IL-2, None) , ( protein: TCEd, None) , ( DNA: IL-2 promoter, None) , ( DNA: transcription factor binding sites, None) , ( protein: distal nuclear factor (NF)-AT, None) " }

[]

{ "id": "554", "sentence": "In EBV-transformed B clones , the chi B site exerted the strongest inducible activity ; the NF-AT binding sites showed either no or only weak activity compared to Jurkat T cells .", "label": " ( cell line: Jurkat T cells, None) , ( DNA: NF-AT binding sites, None) , ( DNA: chi B site, None) , ( cell line: EBV-transformed B clones, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell line: Jurkat T cells, None) , ( DNA: NF-AT binding sites, None) , ( DNA: chi B site, None) , ( cell line: EBV-transformed B clones, None) " }

[]

{ "id": "555", "sentence": "An IL-2 promoter bearing a defective NF- chi B site was completely inactive in EBV-transformed B cells , while it still had activity in Jurkat T cells .", "label": " ( cell line: Jurkat T cells, None) , ( DNA: IL-2 promoter, None) , ( protein: NF- chi B, None) , ( cell line: EBV-transformed B cells, None) , ( DNA: NF- chi B site, None) , ( DNA: chi B, None) , ( cell type: B cells, None) , ( protein: IL-2, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell line: Jurkat T cells, None) , ( DNA: IL-2 promoter, None) , ( protein: NF- chi B, None) , ( cell line: EBV-transformed B cells, None) , ( DNA: NF- chi B site, None) , ( DNA: chi B, None) , ( cell type: B cells, None) , ( protein: IL-2, None) " }

[]

{ "id": "556", "sentence": "In seven EBV-B cell clones or lines differing in their capacity to secrete IL-2 , the activity of the IL-2 promoter correlated well with the status of IL-2 secretion .", "label": " ( protein: IL-2, None) , ( protein: IL-2, None) , ( protein: IL-2, None) , ( cell line: EBV-B cell clones, None) , ( DNA: IL-2 promoter, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: IL-2, None) , ( protein: IL-2, None) , ( protein: IL-2, None) , ( cell line: EBV-B cell clones, None) , ( DNA: IL-2 promoter, None) " }

[]

{ "id": "557", "sentence": "Similarly, a human immunodeficiency virus promoter , whose activity is controlled through chi B factors , was found to be active in the IL-2 producing EBV- B cells , but inactive in the non- IL-2 -producing cells .", "label": " ( cell line: IL-2 producing EBV- B cells, None) , ( cell line: non- IL-2 -producing cells, None) , ( DNA: human immunodeficiency virus promoter, None) , ( protein: IL-2, None) , ( protein: IL-2, None) , ( protein: chi B factors, None) , ( cell type: B cells, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell line: IL-2 producing EBV- B cells, None) , ( cell line: non- IL-2 -producing cells, None) , ( DNA: human immunodeficiency virus promoter, None) , ( protein: IL-2, None) , ( protein: IL-2, None) , ( protein: chi B factors, None) , ( cell type: B cells, None) " }

[]

{ "id": "558", "sentence": "Electrophoretic mobility shift assays using protein extracts from EBV- B cells and the IL-2 NF-chi B probe revealed the constitutive generation of chi B complexes in IL-2 -secreting cells consisting mainly of heterodimeric p50/p65 complexes .", "label": " ( cell line: IL-2 -secreting cells, None) , ( protein: chi B complexes, None) , ( protein: heterodimeric p50/p65 complexes, None) , ( cell line: EBV- B cells, None) , ( protein: NF-chi B, None) , ( protein: IL-2, None) , ( protein: IL-2, None) , ( cell type: B cells, None) , ( protein: protein extracts, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell line: IL-2 -secreting cells, None) , ( protein: chi B complexes, None) , ( protein: heterodimeric p50/p65 complexes, None) , ( cell line: EBV- B cells, None) , ( protein: NF-chi B, None) , ( protein: IL-2, None) , ( protein: IL-2, None) , ( cell type: B cells, None) , ( protein: protein extracts, None) " }

[]

{ "id": "559", "sentence": "A weaker chi B complex formation and faster-migrating complexes were detected in non- IL-2 -secreting cells .", "label": " ( protein: IL-2, None) , ( DNA: chi B, None) , ( cell line: non- IL-2 -secreting cells, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: IL-2, None) , ( DNA: chi B, None) , ( cell line: non- IL-2 -secreting cells, None) " }

[]

{ "id": "560", "sentence": "These results demonstrate that the IL-2 NF-chi B site is indispensable for the activity of the IL-2 promoter in EBV-transformed B cells , whereas other transcription factors appear to be less important for IL-2 expression in these cells.", "label": " ( DNA: IL-2 promoter, None) , ( cell line: EBV-transformed B cells, None) , ( cell type: B cells, None) , ( protein: IL-2, None) , ( protein: IL-2, None) , ( protein: transcription factors, None) , ( protein: IL-2, None) , ( DNA: IL-2 NF-chi B site, None) , ( protein: NF-chi B, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: IL-2 promoter, None) , ( cell line: EBV-transformed B cells, None) , ( cell type: B cells, None) , ( protein: IL-2, None) , ( protein: IL-2, None) , ( protein: transcription factors, None) , ( protein: IL-2, None) , ( DNA: IL-2 NF-chi B site, None) , ( protein: NF-chi B, None) " }

[]

{ "id": "561", "sentence": "Latent membrane protein-1 induces cyclin D2 expression , pRb hyperphosphorylation , and loss of TGF-beta 1 -mediated growth inhibition in EBV-positive B cells .", "label": " ( protein: cyclin D2, None) , ( cell type: EBV-positive B cells, None) , ( protein: TGF-beta 1, None) , ( cell type: B cells, None) , ( protein: pRb, None) , ( protein: Latent membrane protein-1, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: cyclin D2, None) , ( cell type: EBV-positive B cells, None) , ( protein: TGF-beta 1, None) , ( cell type: B cells, None) , ( protein: pRb, None) , ( protein: Latent membrane protein-1, None) " }

[]

{ "id": "562", "sentence": "The normal cell cycle is regulated by several molecules, such as the tumor-suppressor protein pRb , the G1 cyclins , the cyclin-dependent kinases , and their inhibitors .", "label": " ( protein: pRb, None) , ( protein: G1 cyclins, None) , ( protein: tumor-suppressor protein pRb, None) , ( protein: cyclin-dependent kinases, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: pRb, None) , ( protein: G1 cyclins, None) , ( protein: tumor-suppressor protein pRb, None) , ( protein: cyclin-dependent kinases, None) " }

[]

{ "id": "563", "sentence": "These regulators are targeted by negative growth regulatory signals , such as that provided by TGF-beta .", "label": " ( protein: TGF-beta, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: TGF-beta, None) " }

[]

{ "id": "564", "sentence": "Here, we show that the presence of either wild-type EBV or its transforming latent membrane protein-1 ( LMP-1 ) results in the loss of TGF-beta 1 -mediated growth inhibition in human B cells .", "label": " ( cell type: B cells, None) , ( protein: transforming latent membrane protein-1, None) , ( cell type: human B cells, None) , ( protein: TGF-beta 1, None) , ( protein: TGF-beta, None) , ( protein: LMP-1, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell type: B cells, None) , ( protein: transforming latent membrane protein-1, None) , ( cell type: human B cells, None) , ( protein: TGF-beta 1, None) , ( protein: TGF-beta, None) , ( protein: LMP-1, None) " }

[]

{ "id": "565", "sentence": "Chemical cross-linking with 125I-labeled TGF-beta 1 showed an essentially normal TGF-beta receptor profile in EBV-positive and EBV-negative Burkitt's lymphoma cell lines , and these receptors were shown to be functional in transducing signals , as evidenced by the TGF-beta 1 -mediated modulation of junB gene expression .", "label": " ( protein: TGF-beta, None) , ( DNA: junB gene, None) , ( protein: TGF-beta receptor, None) , ( protein: 125I-labeled TGF-beta 1, None) , ( protein: TGF-beta 1, None) , ( protein: TGF-beta 1, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: TGF-beta, None) , ( DNA: junB gene, None) , ( protein: TGF-beta receptor, None) , ( protein: 125I-labeled TGF-beta 1, None) , ( protein: TGF-beta 1, None) , ( protein: TGF-beta 1, None) " }

[]

{ "id": "566", "sentence": "However, TGF-beta 1 did not induce dephosphorylation of pRb in EBV (or LMP-1 )-positive cells as opposed to EBV -negative cells , suggesting a dichotomy in the TGF-beta 1 signaling pathway leading to separable gene regulatory and growth inhibitory responses .", "label": " ( cell line: EBV -negative cells, None) , ( protein: TGF-beta 1, None) , ( cell line: EBV (or LMP-1 )-positive cells, None) , ( protein: TGF-beta 1, None) , ( protein: LMP-1, None) , ( protein: pRb, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell line: EBV -negative cells, None) , ( protein: TGF-beta 1, None) , ( cell line: EBV (or LMP-1 )-positive cells, None) , ( protein: TGF-beta 1, None) , ( protein: LMP-1, None) , ( protein: pRb, None) " }

[]

{ "id": "567", "sentence": "Furthermore, LMP-1 was found to induce the expression of cyclin D2 ; normal B cells or EBV -negative Burkitt's lymphoma cells do not express D-type cyclins .", "label": " ( protein: D-type cyclins, None) , ( protein: cyclin D2, None) , ( cell line: EBV -negative Burkitt#$%#s lymphoma cells, None) , ( cell type: B cells, None) , ( protein: LMP-1, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: D-type cyclins, None) , ( protein: cyclin D2, None) , ( cell line: EBV -negative Burkitt#$%#s lymphoma cells, None) , ( cell type: B cells, None) , ( protein: LMP-1, None) " }

[]

{ "id": "568", "sentence": "Taken together, these data point to a potential mechanism underlying EBV -mediated B cell transformation whereby constitutive induction of key cell cycle regulators by LMP-1 can lead to pRb hyperphosphorylation and uncontrolled cell proliferation .", "label": " ( protein: key cell cycle regulators, None) , ( protein: LMP-1, None) , ( protein: pRb, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: key cell cycle regulators, None) , ( protein: LMP-1, None) , ( protein: pRb, None) " }

[]

{ "id": "569", "sentence": "Does thyroidectomy , radioactive iodine therapy , or antithyroid drug treatment alter reactivity of patients' T cells to epitopes of thyrotropin receptor in autoimmune thyroid diseases ?", "label": " ( protein: thyrotropin receptor, None) , ( cell type: T cells, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: thyrotropin receptor, None) , ( cell type: T cells, None) " }

[]

{ "id": "570", "sentence": "The effect of treatment on thyroid antibody production and T cell reactivity to thyroid antigens was studied in 15 patients with Graves' disease ( GD ) before and after thyroidectomy , 19 patients with GD before and after radioactive iodine (RAI) therapy, and 9 patients maintained euthyroid on antithyroid drugs ( ATD ).", "label": " None", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " None" }

[]

{ "id": "571", "sentence": "Twenty subjects matched for age and sex without known thyroid disease served as controls.", "label": " None", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " None" }

[]

{ "id": "572", "sentence": "In GD patients , the responses of peripheral blood mononuclear cells ( PBMC ) and TSH receptor ( TSHR )-specific T cell lines to recombinant human TSHR extracellular domain , thyroglobulin , and TSHR peptides were examined on the day of surgery or RAI therapy (day 0) and also 6-8 weeks and 3-6 months thereafter.", "label": " ( protein: recombinant human TSHR extracellular domain, None) , ( protein: TSHR, None) , ( protein: thyroglobulin, None) , ( cell line: TSH receptor ( TSHR )-specific T cell lines, None) , ( protein: TSHR, None) , ( cell type: peripheral blood mononuclear cells, None) , ( cell type: PBMC, None) , ( protein: TSH receptor, None) , ( protein: TSHR, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: recombinant human TSHR extracellular domain, None) , ( protein: TSHR, None) , ( protein: thyroglobulin, None) , ( cell line: TSH receptor ( TSHR )-specific T cell lines, None) , ( protein: TSHR, None) , ( cell type: peripheral blood mononuclear cells, None) , ( cell type: PBMC, None) , ( protein: TSH receptor, None) , ( protein: TSHR, None) " }

[]

{ "id": "573", "sentence": "Reactivity to TSHR peptides before surgery was heterogeneous and spanned the entire extracellular domain .", "label": " ( protein: extracellular domain, None) , ( protein: TSHR, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: extracellular domain, None) , ( protein: TSHR, None) " }

[]

{ "id": "574", "sentence": "Six to 8 weeks after subtotal thyroidectomy , the number of patients ' PBMC responding to any peptide and the average number of recognized peptides decreased.", "label": " ( cell type: PBMC, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell type: PBMC, None) " }

[]

{ "id": "575", "sentence": "A further decrease in the T cell reactivity to TSHR peptides was observed 3-6 months after surgery .", "label": " ( protein: TSHR, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: TSHR, None) " }

[]

{ "id": "576", "sentence": "The responses of PBMC from Graves' patients before RAI therapy were less than those in the presurgical group.", "label": " ( cell type: PBMC, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell type: PBMC, None) " }

[]

{ "id": "577", "sentence": "Six to 8 weeks after RAI therapy , the number of patients responding to any peptide and the average number of recognized peptides increased.", "label": " None", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " None" }

[]

{ "id": "578", "sentence": "Three to 6 months after RAI , T cell responses to TSHR peptides were less than those 6-8 weeks after RAI therapy , but still higher than the values on day 0.", "label": " ( protein: TSHR, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: TSHR, None) " }

[]

{ "id": "579", "sentence": "Responses of PBMC from patients with GD , maintained euthyroid on ATD , were lower than those before surgery or RAI therapy .", "label": " ( cell type: PBMC, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell type: PBMC, None) " }

[]

{ "id": "580", "sentence": "The reactivity of T cell lines in different groups reflected a pattern similar to PBMC after treatment.", "label": " ( cell line: T cell lines, None) , ( cell type: PBMC, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell line: T cell lines, None) , ( cell type: PBMC, None) " }

[]

{ "id": "581", "sentence": "TSHR antibody and microsomal antibody levels decreased after surgery , but increased after RAI therapy .", "label": " ( protein: microsomal antibody, None) , ( protein: TSHR, None) , ( protein: TSHR antibody, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: microsomal antibody, None) , ( protein: TSHR, None) , ( protein: TSHR antibody, None) " }

[]

{ "id": "582", "sentence": "The difference in the number of recognized peptides by patients ' PBMC before RAI and surgery may reflect the effect of long term therapy with ATD in the patients before RAI vs. the shorter period in patients before surgery .", "label": " ( cell type: PBMC, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell type: PBMC, None) " }

[]

{ "id": "583", "sentence": "The decreased T cell reactivity to thyroid antigens after thyroidectomy could be the result of removal of a major part of the thyroid gland or redistribution of suppressor-inducer T cells .", "label": " ( cell type: T cells, None) , ( protein: thyroid antigens, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell type: T cells, None) , ( protein: thyroid antigens, None) " }

[]

{ "id": "584", "sentence": "The increased T cell response after RAI therapy is probably epitope specific , rather than a response to the whole TSHR molecule.", "label": " ( protein: TSHR, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: TSHR, None) " }

[]

{ "id": "585", "sentence": "Synchronous recognition of peptides 158-176 and 248-263 is important for the development of GD , and the loss of recognition of one of these epitopes may be an early sign of immune remission and a predictor of euthyroidism .", "label": " ( protein: epitopes, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: epitopes, None) " }

[]

{ "id": "586", "sentence": "Circumvention of tolerance for the nuclear T cell protein TCF-1 by immunization of TCF-1 knock-out mice .", "label": " ( protein: nuclear T cell protein, None) , ( protein: TCF-1, None) , ( protein: TCF-1, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: nuclear T cell protein, None) , ( protein: TCF-1, None) , ( protein: TCF-1, None) " }

[]

{ "id": "587", "sentence": "Molecular events that underlie the well-defined phenotypic changes of the differentiating thymocyte are poorly understood.", "label": " ( cell type: differentiating thymocyte, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell type: differentiating thymocyte, None) " }

[]

{ "id": "588", "sentence": "A candidate gene to control thymocyte differentiation , T cell factor-1 ( TCF-1 )* encodes a DNA-binding protein .", "label": " ( protein: TCF-1, None) , ( protein: T cell factor-1, None) , ( protein: DNA-binding protein, None) , ( cell type: thymocyte, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: TCF-1, None) , ( protein: T cell factor-1, None) , ( protein: DNA-binding protein, None) , ( cell type: thymocyte, None) " }

[]

{ "id": "589", "sentence": "Its mRNA expression pattern is complex during embryogenesis , yet restricted to lymphocytes postnatally.", "label": " ( cell type: lymphocytes, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell type: lymphocytes, None) " }

[]

{ "id": "590", "sentence": "Expression studies on TCF-1 protein have been hampered by the difficulty to raise antibodies due to extreme evolutionary conservation.", "label": " ( protein: TCF-1, None) , ( protein: TCF-1 protein, None) , ( protein: antibodies, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: TCF-1, None) , ( protein: TCF-1 protein, None) , ( protein: antibodies, None) " }

[]

{ "id": "591", "sentence": "TCF-1 knock-out mice , generated recently in our laboratory, have strongly decreased numbers of thymocytes , but are otherwise normal.", "label": " ( protein: TCF-1, None) , ( cell type: thymocytes, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: TCF-1, None) , ( cell type: thymocytes, None) " }

[]

{ "id": "592", "sentence": "We have used these mice to generate anti- TCF-1 antibodies .", "label": " ( protein: anti- TCF-1 antibodies, None) , ( protein: TCF-1, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: anti- TCF-1 antibodies, None) , ( protein: TCF-1, None) " }

[]

{ "id": "593", "sentence": "By immunization with a recombinant fusion protein , we show that TCF-1 knock-out mice readily yield antiserum titers against human and mouse TCF-1 protein .", "label": " ( protein: TCF-1, None) , ( protein: recombinant fusion protein, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: TCF-1, None) , ( protein: recombinant fusion protein, None) " }

[]

{ "id": "594", "sentence": "Wild-type littermates remain unresponsive to TCF-1 while they mount a high-titer antibody response to the fusion protein , Maltose Binding Protein ( MBP ).", "label": " ( protein: Maltose Binding Protein, None) , ( protein: MBP, None) , ( protein: fusion protein, None) , ( protein: TCF-1, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: Maltose Binding Protein, None) , ( protein: MBP, None) , ( protein: fusion protein, None) , ( protein: TCF-1, None) " }

[]

{ "id": "595", "sentence": "Subsequently, TCF-1 -specific hybridomas could be prepared from the spleens of immunized knock-out mice .", "label": " ( cell line: TCF-1 -specific hybridomas, None) , ( protein: TCF-1, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell line: TCF-1 -specific hybridomas, None) , ( protein: TCF-1, None) " }

[]

{ "id": "596", "sentence": "This study illustrates the almost complete tolerance of mice for human TCF-1 and demonstrates that this tolerance is readily broken by gene knock-out .", "label": " ( protein: human TCF-1, None) , ( protein: TCF-1, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: human TCF-1, None) , ( protein: TCF-1, None) " }

[]

{ "id": "597", "sentence": "Furthermore, the usefulness of knock-out mice for the generation of monoclonal antibodies against the gene product of interest is underscored.", "label": " ( protein: monoclonal antibodies, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: monoclonal antibodies, None) " }

[]

{ "id": "598", "sentence": "The transcription factor , Nm23H2 , binds to and activates the translocated c-myc allele in Burkitt's lymphoma .", "label": " ( protein: transcription factor, None) , ( DNA: c-myc allele, None) , ( protein: Nm23H2, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: transcription factor, None) , ( DNA: c-myc allele, None) , ( protein: Nm23H2, None) " }

[]

{ "id": "599", "sentence": "We have identified an in vivo footprint over the PuF site on the translocated c-myc allele in Burkitt's lymphoma cells.", "label": " ( DNA: PuF site, None) , ( DNA: c-myc allele, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: PuF site, None) , ( DNA: c-myc allele, None) " }