Datasets:

huy-nh-2000

/

instructUIE

like 0

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{ "id": "300", "sentence": "MEASUREMENTS: The binding capacity and affinity of the glucocorticoid receptors were measured and compared to clinical data and the plasma cortisol concentrations.", "label": " ( protein: glucocorticoid receptors, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: glucocorticoid receptors, None) " }

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{ "id": "301", "sentence": "RESULTS: Hypercortisolaemia, in vitro, resulted in a decreased affinity and a decreased binding capacity of the glucocorticoid receptor .", "label": " ( protein: glucocorticoid receptor, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: glucocorticoid receptor, None) " }

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{ "id": "302", "sentence": "In vitro, hyperthermia as well as variations in the cellular composition did not influence the glucocorticoid receptor.", "label": " None", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " None" }

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{ "id": "303", "sentence": "In vivo, there was no change in the number of receptors per cell in patients with sepsis or septic shock as compared to healthy controls .", "label": " None", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " None" }

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{ "id": "304", "sentence": "However, a decreased affinity of the glucocorticoid receptor was observed.", "label": " ( protein: glucocorticoid receptor, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: glucocorticoid receptor, None) " }

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{ "id": "305", "sentence": "There was a weak but significant negative correlation between body temperature and the number of glucocorticoid receptors in the patient group.", "label": " ( protein: glucocorticoid receptors, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: glucocorticoid receptors, None) " }

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{ "id": "306", "sentence": "There was no relation between circulating cortisol concentrations and glucocorticoid receptor affinity and number.", "label": " ( protein: glucocorticoid receptor, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: glucocorticoid receptor, None) " }

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{ "id": "307", "sentence": "CONCLUSIONS: There is no obvious regulation of the number of glucocorticoid receptors by plasma cortisol concentrations in vivo.", "label": " ( protein: glucocorticoid receptors, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: glucocorticoid receptors, None) " }

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{ "id": "308", "sentence": "The decreased affinity of the glucocorticoid receptor together with the negative correlation between hyperthermia and the number of glucocorticoid receptors in patients with sepsis or septic shock suggest that hypothalamic-pituitary-adrenal axis activation during critical illness is accompanied by peripheral adaptation in glucocorticoid receptor number and affinity.", "label": " ( protein: glucocorticoid receptor, None) , ( protein: glucocorticoid receptors, None) , ( protein: glucocorticoid receptor, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: glucocorticoid receptor, None) , ( protein: glucocorticoid receptors, None) , ( protein: glucocorticoid receptor, None) " }

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{ "id": "309", "sentence": "Integrin-mediated tyrosine phosphorylation and cytokine message induction in monocytic cells .", "label": " ( protein: cytokine, None) , ( cell type: monocytic cells, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: cytokine, None) , ( cell type: monocytic cells, None) " }

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{ "id": "310", "sentence": "A possible signaling role for the Syk tyrosine kinase .", "label": " ( protein: Syk tyrosine kinase, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: Syk tyrosine kinase, None) " }

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{ "id": "311", "sentence": "Activation of cytoplasmic tyrosine kinases is an important aspect of signal transduction mediated by integrins .", "label": " ( protein: integrins, None) , ( protein: cytoplasmic tyrosine kinases, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: integrins, None) , ( protein: cytoplasmic tyrosine kinases, None) " }

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{ "id": "312", "sentence": "In the human monocytic cell line THP-1 , either integrin -dependent cell adhesion to fibronectin or ligation of beta 1 integrins with antibodies causes a rapid and intense tyrosine phosphorylation of two sets of proteins of about 65-75 and 120-125 kDa .", "label": " ( protein: proteins, None) , ( protein: fibronectin, None) , ( protein: antibodies, None) , ( protein: beta 1 integrins, None) , ( cell line: human monocytic cell line THP-1, None) , ( protein: integrin, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: proteins, None) , ( protein: fibronectin, None) , ( protein: antibodies, None) , ( protein: beta 1 integrins, None) , ( cell line: human monocytic cell line THP-1, None) , ( protein: integrin, None) " }

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{ "id": "313", "sentence": "In addition, integrin ligation leads to nuclear translocation of the p50 and p65 subunits of the NF-kappa B transcription factor , to activation of a reporter gene driven by a promoter containing NF-kappa B sites , and to increased levels of mRNAs for immediate-early genes , including the cytokine interleukin (IL)-1 beta .", "label": " ( DNA: promoter, None) , ( DNA: reporter gene, None) , ( RNA: mRNAs, None) , ( protein: NF-kappa B transcription factor, None) , ( DNA: immediate-early genes, None) , ( DNA: NF-kappa B sites, None) , ( protein: p65 subunits, None) , ( protein: p50, None) , ( protein: cytokine interleukin (IL)-1 beta, None) , ( protein: integrin, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: promoter, None) , ( DNA: reporter gene, None) , ( RNA: mRNAs, None) , ( protein: NF-kappa B transcription factor, None) , ( DNA: immediate-early genes, None) , ( DNA: NF-kappa B sites, None) , ( protein: p65 subunits, None) , ( protein: p50, None) , ( protein: cytokine interleukin (IL)-1 beta, None) , ( protein: integrin, None) " }

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{ "id": "314", "sentence": "The tyrosine kinase inhibitors genistein and herbimycin A block both integrin -mediated tyrosine phosphorylation and increases in IL-1 beta message levels , indicating a causal relationship between the two events.", "label": " ( protein: integrin, None) , ( protein: IL-1 beta, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: integrin, None) , ( protein: IL-1 beta, None) " }

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{ "id": "315", "sentence": "The components tyrosine phosphorylated subsequent to cell adhesion include paxillin , pp125FAK , and the SH2 domain containing tyrosine kinase Syk .", "label": " ( protein: SH2 domain containing tyrosine kinase Syk, None) , ( protein: SH2 domain, None) , ( protein: paxillin, None) , ( protein: pp125FAK, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: SH2 domain containing tyrosine kinase Syk, None) , ( protein: SH2 domain, None) , ( protein: paxillin, None) , ( protein: pp125FAK, None) " }

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{ "id": "316", "sentence": "In contrast, integrin ligation with antibodies induces tyrosine phosphorylation of Syk but not of FAK or paxillin .", "label": " ( protein: integrin, None) , ( protein: FAK, None) , ( protein: Syk, None) , ( protein: antibodies, None) , ( protein: paxillin, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: integrin, None) , ( protein: FAK, None) , ( protein: Syk, None) , ( protein: antibodies, None) , ( protein: paxillin, None) " }

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{ "id": "317", "sentence": "In adhering cells , pre-treatment with cytochalasin D suppresses tyrosine phosphorylation of FAK and paxillin but not of Syk , while IL-1 beta message induction is unaffected.", "label": " ( protein: IL-1 beta, None) , ( protein: paxillin, None) , ( cell type: adhering cells, None) , ( protein: FAK, None) , ( protein: Syk, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: IL-1 beta, None) , ( protein: paxillin, None) , ( cell type: adhering cells, None) , ( protein: FAK, None) , ( protein: Syk, None) " }

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{ "id": "318", "sentence": "These observations indicate that the Syk tyrosine kinase may be an important component of an integrin signaling pathway in monocytic cells , leading to activation of NF-kappa B and to increased levels of cytokine messages .", "label": " ( protein: integrin, None) , ( protein: Syk, None) , ( cell type: monocytic cells, None) , ( protein: NF-kappa B, None) , ( protein: cytokine, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: integrin, None) , ( protein: Syk, None) , ( cell type: monocytic cells, None) , ( protein: NF-kappa B, None) , ( protein: cytokine, None) " }

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{ "id": "319", "sentence": "Inhibition of dexamethasone binding to human glucocorticoid receptor by New World primate cell extracts .", "label": " ( protein: human glucocorticoid receptor, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: human glucocorticoid receptor, None) " }

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{ "id": "320", "sentence": "To determine if New World primates express an inhibitor that influences glucocorticoid receptor ( GR ) binding characteristics , we examined [3H]dexamethasone binding in cytosol prepared from B95-8 lymphoid cells , derived from the cotton top tamarin ( Saguinus oedipus ), in combination with cytosol prepared from human or rat tissues .", "label": " ( protein: glucocorticoid receptor, None) , ( protein: GR, None) , ( cell line: B95-8 lymphoid cells, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: glucocorticoid receptor, None) , ( protein: GR, None) , ( cell line: B95-8 lymphoid cells, None) " }

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{ "id": "321", "sentence": "B95-8 cytosol inhibited specific binding of [3H]dexamethasone (P < 0.01) when mixed with cytosol prepared from either a human lymphoid cell line ( HL ) or rat thymus .", "label": " ( cell line: HL, None) , ( cell line: human lymphoid cell line, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell line: HL, None) , ( cell line: human lymphoid cell line, None) " }

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{ "id": "322", "sentence": "The inhibitory activity was heat labile and trypsin sensitive .", "label": " ( protein: trypsin, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: trypsin, None) " }

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{ "id": "323", "sentence": "Peak inhibitory activity was found in the 150-200 kd fractions after Sephacryl G-200 ultrafiltration .", "label": " None", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " None" }

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{ "id": "324", "sentence": "Scatchard analysis of [3H]dexamethasone binding using mixed cytosol showed a diminished GR apparent binding affinity when compared to HL cytosol .", "label": " ( protein: GR, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: GR, None) " }

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{ "id": "325", "sentence": "Kinetic studies using mixed cytosol indicated that B95-8 cytosol did not affect the apparent dissociation rate of [3H]dexamethasone .", "label": " None", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " None" }

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{ "id": "326", "sentence": "These data demonstrate that B95-8 cells contain a competitive inhibitor that prevents binding of dexamethasone to its cognate receptor .", "label": " ( cell type: B95-8 cells, None) , ( protein: cognate receptor, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell type: B95-8 cells, None) , ( protein: cognate receptor, None) " }

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{ "id": "327", "sentence": "Disruption of a GATA motif in the Duffy gene promoter abolishes erythroid gene expression in Duffy-negative individuals .", "label": " ( DNA: GATA motif, None) , ( DNA: erythroid gene, None) , ( DNA: Duffy gene promoter, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: GATA motif, None) , ( DNA: erythroid gene, None) , ( DNA: Duffy gene promoter, None) " }

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{ "id": "328", "sentence": "The mRNA for the Duffy blood group antigen , the erythrocyte receptor for the Plasmodium vivax malaria parasite , has recently been cloned and shown to encode a widely expressed chemokine receptor .", "label": " ( protein: chemokine receptor, None) , ( protein: Duffy blood group antigen, None) , ( RNA: mRNA, None) , ( protein: erythrocyte receptor, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: chemokine receptor, None) , ( protein: Duffy blood group antigen, None) , ( RNA: mRNA, None) , ( protein: erythrocyte receptor, None) " }

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{ "id": "329", "sentence": "Here, we show that the Duffy antigen/chemokine receptor gene ( DARC ) is composed of a single exon and that most Duffy-negative blacks carry a silent FY*B allele with a single T to C substitution at nucleotide -46 .", "label": " ( DNA: Duffy antigen/chemokine receptor gene, None) , ( DNA: exon, None) , ( protein: Duffy antigen/chemokine receptor, None) , ( DNA: DARC, None) , ( DNA: silent FY*B allele, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: Duffy antigen/chemokine receptor gene, None) , ( DNA: exon, None) , ( protein: Duffy antigen/chemokine receptor, None) , ( DNA: DARC, None) , ( DNA: silent FY*B allele, None) " }

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{ "id": "330", "sentence": "This mutation impairs the promoter activity in erythroid cells by disrupting a binding site for the GATA1 erythroid transcription factor .", "label": " ( cell type: erythroid cells, None) , ( DNA: binding site, None) , ( protein: GATA1 erythroid transcription factor, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell type: erythroid cells, None) , ( DNA: binding site, None) , ( protein: GATA1 erythroid transcription factor, None) " }

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{ "id": "331", "sentence": "With the recent characterization of the FY*A and FY*B alleles , these findings provide the molecular basis of the Duffy blood group system and an explanation for the erythroid-specific repression of the DARC gene in Duffy-negative individuals .", "label": " ( DNA: DARC gene, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: DARC gene, None) " }

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{ "id": "332", "sentence": "Activation of pp90rsk and early growth response-1 gene expression by pokeweed mitogen in human B cells .", "label": " ( DNA: early growth response-1 gene, None) , ( protein: pokeweed mitogen, None) , ( protein: pp90rsk, None) , ( cell type: human B cells, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: early growth response-1 gene, None) , ( protein: pokeweed mitogen, None) , ( protein: pp90rsk, None) , ( cell type: human B cells, None) " }

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{ "id": "333", "sentence": "The present studies have examined the effects of pokeweed mitogen ( PWM ) on the induction of early growth response-1 gene ( EGR-1 ) in normal human B cells .", "label": " ( protein: pokeweed mitogen, None) , ( cell type: human B cells, None) , ( cell type: normal human B cells, None) , ( DNA: EGR-1, None) , ( DNA: early growth response-1 gene, None) , ( protein: PWM, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: pokeweed mitogen, None) , ( cell type: human B cells, None) , ( cell type: normal human B cells, None) , ( DNA: EGR-1, None) , ( DNA: early growth response-1 gene, None) , ( protein: PWM, None) " }

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{ "id": "334", "sentence": "PWM regulates EGR-1 gene expression by both transcriptional and post-transcriptional mechanisms .", "label": " ( DNA: EGR-1, None) , ( protein: PWM, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: EGR-1, None) , ( protein: PWM, None) " }

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{ "id": "335", "sentence": "Transient transfection assays with EGR-1 promoter fragments linked to the chloramphenicol acetyltransferase ( CAT ) gene demonstrated that PWM induced EGR-1 transcription is conferred by the CArG motif ( C C[AT]6GG ) in the EGR-1 promoter .", "label": " ( protein: PWM, None) , ( protein: chloramphenicol acetyltransferase, None) , ( DNA: EGR-1, None) , ( DNA: EGR-1 promoter, None) , ( DNA: EGR-1, None) , ( DNA: chloramphenicol acetyltransferase ( CAT ) gene, None) , ( DNA: EGR-1, None) , ( DNA: CArG motif, None) , ( DNA: C C[AT]6GG, None) , ( protein: CAT, None) , ( DNA: EGR-1 promoter fragments, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: PWM, None) , ( protein: chloramphenicol acetyltransferase, None) , ( DNA: EGR-1, None) , ( DNA: EGR-1 promoter, None) , ( DNA: EGR-1, None) , ( DNA: chloramphenicol acetyltransferase ( CAT ) gene, None) , ( DNA: EGR-1, None) , ( DNA: CArG motif, None) , ( DNA: C C[AT]6GG, None) , ( protein: CAT, None) , ( DNA: EGR-1 promoter fragments, None) " }

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{ "id": "336", "sentence": "The results further demonstrated the activation of S6 kinase ( pp90rsk ), evidenced by phosphorylation of S6 and serum response factor (SRF) peptides , in PWM treated B cells .", "label": " ( protein: PWM, None) , ( cell line: PWM treated B cells, None) , ( protein: S6 kinase, None) , ( protein: S6, None) , ( protein: pp90rsk, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: PWM, None) , ( cell line: PWM treated B cells, None) , ( protein: S6 kinase, None) , ( protein: S6, None) , ( protein: pp90rsk, None) " }

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{ "id": "337", "sentence": "Taken together, these findings suggest that PWM is able to initiate an intracytoplasmic signalling cascade and EGR-1 induction in normal human B cells .", "label": " ( cell type: normal human B cells, None) , ( cell type: human B cells, None) , ( DNA: EGR-1, None) , ( protein: PWM, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell type: normal human B cells, None) , ( cell type: human B cells, None) , ( DNA: EGR-1, None) , ( protein: PWM, None) " }

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{ "id": "338", "sentence": "A conserved motif in the promoters of several cytokines expressed by human Th2-type lymphocytes .", "label": " ( DNA: conserved motif, None) , ( DNA: promoters, None) , ( cell line: human Th2-type lymphocytes, None) , ( protein: cytokines, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: conserved motif, None) , ( DNA: promoters, None) , ( cell line: human Th2-type lymphocytes, None) , ( protein: cytokines, None) " }

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{ "id": "339", "sentence": "We have recently found a novel conserved motif in the promoters of several T-cell-expressed cytokines [ human interleukin -2 , -4 , -5 and -13 and human and mouse granulocyte/macrophage-colony stimulating factor ( GM-CSF )].", "label": " ( protein: T-cell-expressed cytokines, None) , ( protein: GM-CSF, None) , ( DNA: promoters, None) , ( protein: cytokines, None) , ( DNA: conserved motif, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: T-cell-expressed cytokines, None) , ( protein: GM-CSF, None) , ( DNA: promoters, None) , ( protein: cytokines, None) , ( DNA: conserved motif, None) " }

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{ "id": "340", "sentence": "It contains a core sequence CTTGG ... CCAAG which is present as part of larger palindromic sequences in each gene.", "label": " ( DNA: core sequence, None) , ( DNA: palindromic sequences, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: core sequence, None) , ( DNA: palindromic sequences, None) " }

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{ "id": "341", "sentence": "This suggest that they may interact with a new family of trans-acting factors .", "label": " ( protein: trans-acting factors, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: trans-acting factors, None) " }

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{ "id": "342", "sentence": "In transfection assays , the human GM-CSF element has a strong positive effect on the expression of a reporter gene by the human T cell line Jurkat J6 upon stimulation .", "label": " ( cell line: Jurkat J6, None) , ( DNA: human GM-CSF element, None) , ( protein: GM-CSF, None) , ( cell line: human T cell line, None) , ( DNA: reporter gene, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell line: Jurkat J6, None) , ( DNA: human GM-CSF element, None) , ( protein: GM-CSF, None) , ( cell line: human T cell line, None) , ( DNA: reporter gene, None) " }

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{ "id": "343", "sentence": "In DNA mobility shift assays , this sequence can give either six different specific bands which are competed out by different parts of the sequence or one specific band which is competed out by each of the inverted repeats, depending on the reconstitution conditions .", "label": " None", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " None" }

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{ "id": "344", "sentence": "In different genes , the core sequences are separated by integer numbers of helical turns .", "label": " ( DNA: core sequences, None) , ( DNA: helical turns, None) , ( DNA: genes, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: core sequences, None) , ( DNA: helical turns, None) , ( DNA: genes, None) " }

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{ "id": "345", "sentence": "Considering the strong positive regulatory effect of this element and its presence in several T-cell-expressed cytokine genes , it may be crucial to the coordinated expression of these cytokines in T helper cells .", "label": " ( DNA: T-cell-expressed cytokine genes, None) , ( protein: cytokines, None) , ( DNA: element, None) , ( cell type: T helper cells, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: T-cell-expressed cytokine genes, None) , ( protein: cytokines, None) , ( DNA: element, None) , ( cell type: T helper cells, None) " }

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{ "id": "346", "sentence": "cDNA cloning of a NGFI-B/nur77-related transcription factor from an apoptotic human T cell line .", "label": " ( cell line: apoptotic human T cell line, None) , ( DNA: cDNA, None) , ( protein: NGFI-B/nur77-related transcription factor, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell line: apoptotic human T cell line, None) , ( DNA: cDNA, None) , ( protein: NGFI-B/nur77-related transcription factor, None) " }

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{ "id": "347", "sentence": "A human T lymphoid cell line , PEER , dies by apoptosis in the presence of PMA and calcium ionophore .", "label": " ( cell line: PEER, None) , ( cell line: human T lymphoid cell line, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell line: PEER, None) , ( cell line: human T lymphoid cell line, None) " }

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{ "id": "348", "sentence": "A new gene , TINUR , was cloned from apoptotic PEER cells .", "label": " ( DNA: gene, None) , ( DNA: TINUR, None) , ( cell line: apoptotic PEER cells, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: gene, None) , ( DNA: TINUR, None) , ( cell line: apoptotic PEER cells, None) " }

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{ "id": "349", "sentence": "The expression of the TINUR gene is induced within 1 h after the cross-linking of the T cell Ag receptor complex .", "label": " ( DNA: TINUR gene, None) , ( protein: T cell Ag receptor complex, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: TINUR gene, None) , ( protein: T cell Ag receptor complex, None) " }

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{ "id": "350", "sentence": "TINUR belongs to the NGFI-B/nur77 family of the steroid receptor superfamily and is an orphan receptor .", "label": " ( DNA: TINUR, None) , ( protein: NGFI-B/nur77 family, None) , ( protein: orphan receptor, None) , ( protein: steroid receptor superfamily, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: TINUR, None) , ( protein: NGFI-B/nur77 family, None) , ( protein: orphan receptor, None) , ( protein: steroid receptor superfamily, None) " }

[]

{ "id": "351", "sentence": "TINUR binds to the same DNA sequence as NGFI-B/nur77 .", "label": " ( DNA: DNA sequence, None) , ( DNA: TINUR, None) , ( protein: NGFI-B/nur77, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: DNA sequence, None) , ( DNA: TINUR, None) , ( protein: NGFI-B/nur77, None) " }

[]

{ "id": "352", "sentence": "We also propose that the NGFI-B/nur77 family can be classified into two subtypes.", "label": " ( protein: NGFI-B/nur77 family, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: NGFI-B/nur77 family, None) " }

[]

{ "id": "353", "sentence": "Aspirin inhibits nuclear factor-kappa B mobilization and monocyte adhesion in stimulated human endothelial cells .", "label": " ( cell line: stimulated human endothelial cells, None) , ( protein: nuclear factor-kappa B, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell line: stimulated human endothelial cells, None) , ( protein: nuclear factor-kappa B, None) " }

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{ "id": "354", "sentence": "BACKGROUND: The induction of vascular cell adhesion molecule-1 ( VCAM-1 ) and E-selectin by tumor necrosis factor-alpha ( TNF ) is mediated by mobilization of the transcription factor nuclear factor-kappa B ( NF-kappa B ).", "label": " ( protein: vascular cell adhesion molecule-1, None) , ( protein: tumor necrosis factor-alpha, None) , ( protein: NF-kappa B, None) , ( protein: nuclear factor-kappa B, None) , ( protein: transcription factor, None) , ( protein: VCAM-1, None) , ( protein: E-selectin, None) , ( protein: TNF, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: vascular cell adhesion molecule-1, None) , ( protein: tumor necrosis factor-alpha, None) , ( protein: NF-kappa B, None) , ( protein: nuclear factor-kappa B, None) , ( protein: transcription factor, None) , ( protein: VCAM-1, None) , ( protein: E-selectin, None) , ( protein: TNF, None) " }

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{ "id": "355", "sentence": "Since salicylates have been reported to inhibit NF-kappa B activation by preventing the degradation of its inhibitor I kappa B , we studied a potential inhibition of this pathway by acetylsalicylate ( aspirin ) in human umbilical vein endothelial cells ( HUVECs ).", "label": " ( protein: I kappa B, None) , ( cell line: HUVECs, None) , ( cell line: human umbilical vein endothelial cells, None) , ( protein: NF-kappa B, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: I kappa B, None) , ( cell line: HUVECs, None) , ( cell line: human umbilical vein endothelial cells, None) , ( protein: NF-kappa B, None) " }

[]

{ "id": "356", "sentence": "METHODS AND RESULTS: Gel-shift analyses demonstrated dose-dependent inhibition of TNF -induced NF-kappa B mobilization by aspirin at concentrations ranging from 1 to 10 mmol/L.", "label": " ( protein: NF-kappa B, None) , ( protein: TNF, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: NF-kappa B, None) , ( protein: TNF, None) " }

[]

{ "id": "357", "sentence": "Induction of VCAM-1 and E-selectin surface expression by TNF was dose-dependently reduced by aspirin over the same range, while induction of intercellular adhesion molecule-1 ( ICAM-1 ) was hardly affected.", "label": " ( protein: E-selectin, None) , ( protein: VCAM-1, None) , ( protein: TNF, None) , ( protein: intercellular adhesion molecule-1, None) , ( protein: ICAM-1, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: E-selectin, None) , ( protein: VCAM-1, None) , ( protein: TNF, None) , ( protein: intercellular adhesion molecule-1, None) , ( protein: ICAM-1, None) " }

[]

{ "id": "358", "sentence": "Aspirin appeared to prevent VCAM-1 transcription , since it dose-dependently inhibited induction of VCAM-1 mRNA by TNF .", "label": " ( protein: VCAM-1, None) , ( protein: TNF, None) , ( RNA: VCAM-1 mRNA, None) , ( protein: VCAM-1, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: VCAM-1, None) , ( protein: TNF, None) , ( RNA: VCAM-1 mRNA, None) , ( protein: VCAM-1, None) " }

[]

{ "id": "359", "sentence": "As a functional consequence, adhesion of U937 monocytes to TNF -stimulated HUVECs was markedly reduced by aspirin due to suppression of VCAM-1 and E-selectin upregulation .", "label": " ( protein: VCAM-1, None) , ( cell line: HUVECs, None) , ( protein: E-selectin, None) , ( cell line: U937 monocytes, None) , ( cell line: TNF -stimulated HUVECs, None) , ( protein: TNF, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: VCAM-1, None) , ( cell line: HUVECs, None) , ( protein: E-selectin, None) , ( cell line: U937 monocytes, None) , ( cell line: TNF -stimulated HUVECs, None) , ( protein: TNF, None) " }

[]

{ "id": "360", "sentence": "These effects of aspirin were not related to the inhibition of cyclooxygenase activity , since indomethacin was ineffective.", "label": " ( protein: cyclooxygenase, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: cyclooxygenase, None) " }

[]

{ "id": "361", "sentence": "CONCLUSIONS: Our data suggest that aspirin inhibits NF-kappa B mobilization , induction of VCAM-1 and E-selectin , and subsequent monocyte adhesion in endothelial cells stimulated by TNF , thereby providing an additional mechanism for therapeutic effects of aspirin .", "label": " ( protein: E-selectin, None) , ( protein: TNF, None) , ( protein: NF-kappa B, None) , ( protein: VCAM-1, None) , ( cell type: endothelial cells, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: E-selectin, None) , ( protein: TNF, None) , ( protein: NF-kappa B, None) , ( protein: VCAM-1, None) , ( cell type: endothelial cells, None) " }

[]

{ "id": "362", "sentence": "Activation of NF-kappa B by phosphatase inhibitors involves the phosphorylation of I kappa B alpha at phosphatase 2A-sensitive sites .", "label": " ( protein: I kappa B alpha, None) , ( protein: phosphatase, None) , ( protein: phosphatase 2A-sensitive sites, None) , ( protein: NF-kappa B, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: I kappa B alpha, None) , ( protein: phosphatase, None) , ( protein: phosphatase 2A-sensitive sites, None) , ( protein: NF-kappa B, None) " }

[]

{ "id": "363", "sentence": "Activation of NF-kappa B by various cellular stimuli involves the phosphorylation and subsequent degradation of its inhibitor, I kappa B alpha , although the underlying mechanism remains unclear.", "label": " ( protein: NF-kappa B, None) , ( protein: I kappa B alpha, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: NF-kappa B, None) , ( protein: I kappa B alpha, None) " }

[]

{ "id": "364", "sentence": "In the present study, the role of serine/threonine phosphatases in the regulation of I kappa B alpha phosphorylation was investigated.", "label": " ( protein: serine/threonine phosphatases, None) , ( protein: I kappa B alpha, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: serine/threonine phosphatases, None) , ( protein: I kappa B alpha, None) " }

[]

{ "id": "365", "sentence": "Our studies demonstrate that incubation of human T cells with low concentrations (approximately 1-5 nM) of calyculin A or okadaic acid , potent inhibitors of protein phosphatase type 1 (PP-1) and type 2A ( PP-2A ), induces the phosphorylation of I kappa B alpha even in the absence of any cellular stimulus.", "label": " ( cell type: human T cells, None) , ( protein: PP-2A, None) , ( protein: I kappa B alpha, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell type: human T cells, None) , ( protein: PP-2A, None) , ( protein: I kappa B alpha, None) " }

[]

{ "id": "366", "sentence": "This action of the phosphatase inhibitors , which is associated with the activation of the RelA.p50 NF-kappa B heterodimer , is not affected by agents that block the induction of I kappa B alpha phosphorylation by tumor necrosis factor alpha ( TNF-alpha ).", "label": " ( protein: NF-kappa B, None) , ( protein: RelA.p50 NF-kappa B heterodimer, None) , ( protein: tumor necrosis factor alpha, None) , ( protein: I kappa B alpha, None) , ( protein: TNF-alpha, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: NF-kappa B, None) , ( protein: RelA.p50 NF-kappa B heterodimer, None) , ( protein: tumor necrosis factor alpha, None) , ( protein: I kappa B alpha, None) , ( protein: TNF-alpha, None) " }

[]

{ "id": "367", "sentence": "Furthermore, the phosphorylated I kappa B alpha from calyculin A -treated cells , but not that from TNF-alpha -stimulated cells , is sensitive to PP-2A in vitro, suggesting the existence of fundamental differences in the phosphorylation of I kappa B alpha induced by the two different NF-kappa B inducers .", "label": " ( protein: TNF-alpha, None) , ( protein: I kappa B alpha, None) , ( cell line: calyculin A -treated cells, None) , ( protein: phosphorylated I kappa B alpha, None) , ( cell line: TNF-alpha -stimulated cells, None) , ( protein: I kappa B alpha, None) , ( protein: PP-2A, None) , ( protein: NF-kappa B, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: TNF-alpha, None) , ( protein: I kappa B alpha, None) , ( cell line: calyculin A -treated cells, None) , ( protein: phosphorylated I kappa B alpha, None) , ( cell line: TNF-alpha -stimulated cells, None) , ( protein: I kappa B alpha, None) , ( protein: PP-2A, None) , ( protein: NF-kappa B, None) " }

[]

{ "id": "368", "sentence": "However, induction of I kappa B alpha phosphorylation by both TNF-alpha and the phosphatase inhibitors is associated with the subsequent degradation of I kappa B alpha .", "label": " ( protein: I kappa B alpha, None) , ( protein: TNF-alpha, None) , ( protein: I kappa B alpha, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: I kappa B alpha, None) , ( protein: TNF-alpha, None) , ( protein: I kappa B alpha, None) " }

[]

{ "id": "369", "sentence": "We further demonstrate that TNF-alpha- and calyculin A-induced I kappa B alpha degradation exhibits similar but not identical sensitivities to a proteasome inhibitor .", "label": " None", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " None" }

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{ "id": "370", "sentence": "Together, these results suggest that phosphorylation of I kappa B alpha , mediated through both the TNF-alpha -inducible and the PP-2A -opposing kinases , may serve to target I kappa B alpha for proteasome -mediated degradation .", "label": " ( protein: I kappa B alpha, None) , ( protein: TNF-alpha, None) , ( protein: I kappa B alpha, None) , ( protein: PP-2A -opposing kinases, None) , ( protein: PP-2A, None) , ( protein: proteasome, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: I kappa B alpha, None) , ( protein: TNF-alpha, None) , ( protein: I kappa B alpha, None) , ( protein: PP-2A -opposing kinases, None) , ( protein: PP-2A, None) , ( protein: proteasome, None) " }

[]

{ "id": "371", "sentence": "Sp1 functions in a chromatin -dependent manner to augment human alpha-globin promoter activity .", "label": " ( DNA: chromatin, None) , ( DNA: human alpha-globin promoter, None) , ( protein: Sp1, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: chromatin, None) , ( DNA: human alpha-globin promoter, None) , ( protein: Sp1, None) " }

[]

{ "id": "372", "sentence": "The 5' flanking region of the human alpha-globin gene is highly G + C rich and contains multiple copies of the consensus sequence for the Sp1 binding site .", "label": " ( DNA: Sp1 binding site, None) , ( DNA: consensus sequence, None) , ( DNA: human alpha-globin gene, None) , ( protein: Sp1, None) , ( DNA: G + C rich, None) , ( DNA: 5#$%# flanking region, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: Sp1 binding site, None) , ( DNA: consensus sequence, None) , ( DNA: human alpha-globin gene, None) , ( protein: Sp1, None) , ( DNA: G + C rich, None) , ( DNA: 5#$%# flanking region, None) " }

[]

{ "id": "373", "sentence": "We investigated the role of this G + C-rich region in augmenting alpha-globin promoter activity in the presence of the far-upstream alpha-globin enhancer , HS-40 .", "label": " ( DNA: HS-40, None) , ( DNA: G + C-rich region, None) , ( DNA: alpha-globin promoter, None) , ( DNA: far-upstream alpha-globin enhancer, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: HS-40, None) , ( DNA: G + C-rich region, None) , ( DNA: alpha-globin promoter, None) , ( DNA: far-upstream alpha-globin enhancer, None) " }

[]

{ "id": "374", "sentence": "We show that in transiently transfected erythroid cells , deletion of the alpha-globin G + C-rich 5' flanking region has no effect on alpha-globin promoter activity .", "label": " ( DNA: alpha-globin promoter, None) , ( DNA: alpha-globin G + C-rich 5#$%# flanking region, None) , ( cell line: transiently transfected erythroid cells, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: alpha-globin promoter, None) , ( DNA: alpha-globin G + C-rich 5#$%# flanking region, None) , ( cell line: transiently transfected erythroid cells, None) " }

[]

{ "id": "375", "sentence": "However, upon stable integration into chromatin , deletion of this region causes a nearly 90% decrease in promoter activity compared with expression from an alpha-globin promoter retaining this region.", "label": " ( DNA: alpha-globin promoter, None) , ( DNA: chromatin, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: alpha-globin promoter, None) , ( DNA: chromatin, None) " }

[]

{ "id": "376", "sentence": "These results suggest that the alpha-globin G + C-rich 5' flanking region augments alpha-globin promoter activity in a chromatin -dependent manner .", "label": " ( DNA: alpha-globin promoter, None) , ( DNA: alpha-globin G + C-rich 5#$%# flanking region, None) , ( DNA: chromatin, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: alpha-globin promoter, None) , ( DNA: alpha-globin G + C-rich 5#$%# flanking region, None) , ( DNA: chromatin, None) " }

[]

{ "id": "377", "sentence": "We further show that this G + C-rich region is required for the activation of alpha-globin gene expression during erythroid differentiation .", "label": " ( DNA: alpha-globin gene, None) , ( DNA: G + C-rich region, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: alpha-globin gene, None) , ( DNA: G + C-rich region, None) " }

[]

{ "id": "378", "sentence": "Finally, we show by both footprint analysis and functional assays that the ability of the G + C-rich region to increase alpha-globin promoter activity from a stably integrated alpha-globin gene is mediated by its multiple binding sites for the transcription factor Sp1 .", "label": " ( protein: transcription factor, None) , ( DNA: alpha-globin gene, None) , ( DNA: multiple binding sites, None) , ( DNA: alpha-globin promoter, None) , ( DNA: G + C-rich region, None) , ( protein: Sp1, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: transcription factor, None) , ( DNA: alpha-globin gene, None) , ( DNA: multiple binding sites, None) , ( DNA: alpha-globin promoter, None) , ( DNA: G + C-rich region, None) , ( protein: Sp1, None) " }

[]

{ "id": "379", "sentence": "Costimulation of human CD4+ T cells with LFA-3 and B7 induce distinct effects on AP-1 and NF-kappa B transcription factors .", "label": " ( protein: LFA-3, None) , ( protein: transcription factors, None) , ( cell line: human CD4+ T cells, None) , ( protein: NF-kappa B, None) , ( protein: AP-1, None) , ( protein: B7, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: LFA-3, None) , ( protein: transcription factors, None) , ( cell line: human CD4+ T cells, None) , ( protein: NF-kappa B, None) , ( protein: AP-1, None) , ( protein: B7, None) " }

[]

{ "id": "380", "sentence": "We have earlier shown that stimulation of human CD4+ T cells with SEA presented on Chinese hamster ovary (CHO)-DR transfectants coexpressing either B7 or LFA-3 resulted in distinct cytokine profiles .", "label": " ( protein: B7, None) , ( protein: cytokine, None) , ( cell line: human CD4+ T cells, None) , ( cell line: Chinese hamster ovary (CHO)-DR transfectants, None) , ( protein: LFA-3, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: B7, None) , ( protein: cytokine, None) , ( cell line: human CD4+ T cells, None) , ( cell line: Chinese hamster ovary (CHO)-DR transfectants, None) , ( protein: LFA-3, None) " }

[]

{ "id": "381", "sentence": "We now demonstrate that B7 , but not LFA-3 , strongly costimulated IL-2 transcription and mRNA expression in CD4+ T cells .", "label": " ( protein: B7, None) , ( protein: LFA-3, None) , ( protein: IL-2, None) , ( cell line: CD4+ T cells, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: B7, None) , ( protein: LFA-3, None) , ( protein: IL-2, None) , ( cell line: CD4+ T cells, None) " }

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{ "id": "382", "sentence": "Maximal increase in IL-2 transcription was recorded with CHO-DR/ B7 / LFA-3 , suggesting a cooperative effect of B7 and LFA-3 at the transcriptional level.", "label": " ( protein: LFA-3, None) , ( protein: IL-2, None) , ( protein: B7, None) , ( protein: CHO-DR/ B7 / LFA-3, None) , ( protein: B7, None) , ( protein: LFA-3, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: LFA-3, None) , ( protein: IL-2, None) , ( protein: B7, None) , ( protein: CHO-DR/ B7 / LFA-3, None) , ( protein: B7, None) , ( protein: LFA-3, None) " }

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{ "id": "383", "sentence": "Gel-shift analysis demonstrated that stimulation of CD4+ T cells with CHO-DR and staphylococcal enterotoxin A was sufficient to induce significant amounts of NF-kappa B binding proteins, whereas induction of AP-1 binding proteins required costimulation.", "label": " ( protein: CHO-DR, None) , ( cell line: CD4+ T cells, None) , ( protein: NF-kappa B, None) , ( protein: AP-1 binding proteins, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: CHO-DR, None) , ( cell line: CD4+ T cells, None) , ( protein: NF-kappa B, None) , ( protein: AP-1 binding proteins, None) " }

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{ "id": "384", "sentence": "LFA-3 induced moderate levels of AP-1 , but did not influence the levels of NF-kappa B , while B7 costimulation strongly induced both AP-1 and substantially enhanced NF-kappa B binding proteins .", "label": " ( protein: AP-1, None) , ( protein: B7, None) , ( protein: NF-kappa B binding proteins, None) , ( protein: LFA-3, None) , ( protein: NF-kappa B, None) , ( protein: NF-kappa B, None) , ( protein: AP-1, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: AP-1, None) , ( protein: B7, None) , ( protein: NF-kappa B binding proteins, None) , ( protein: LFA-3, None) , ( protein: NF-kappa B, None) , ( protein: NF-kappa B, None) , ( protein: AP-1, None) " }

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{ "id": "385", "sentence": "The CHO-DR/ B7 / LFA-3 triple transfectant induced a further increase in AP-1 and NF-kappa B binding proteins compared with the double transfectants .", "label": " ( cell line: CHO-DR/ B7 / LFA-3 triple transfectant, None) , ( cell line: double transfectants, None) , ( protein: LFA-3, None) , ( protein: B7, None) , ( protein: binding proteins, None) , ( protein: AP-1, None) , ( protein: NF-kappa B, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell line: CHO-DR/ B7 / LFA-3 triple transfectant, None) , ( cell line: double transfectants, None) , ( protein: LFA-3, None) , ( protein: B7, None) , ( protein: binding proteins, None) , ( protein: AP-1, None) , ( protein: NF-kappa B, None) " }

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{ "id": "386", "sentence": "The level of Oct-1 binding proteins remained similar in all samples.", "label": " ( protein: Oct-1 binding proteins, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: Oct-1 binding proteins, None) " }

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{ "id": "387", "sentence": "Super-shift analysis revealed that the NF-kappa B complex of costimulated CD4+ T cells contained large amounts of p50 , substantial amounts of p65 , and marginal levels of c-Rel proteins .", "label": " ( cell line: costimulated CD4+ T cells, None) , ( protein: c-Rel proteins, None) , ( protein: NF-kappa B complex, None) , ( protein: p50, None) , ( cell line: CD4+ T cells, None) , ( protein: p65, None) , ( protein: NF-kappa B, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell line: costimulated CD4+ T cells, None) , ( protein: c-Rel proteins, None) , ( protein: NF-kappa B complex, None) , ( protein: p50, None) , ( cell line: CD4+ T cells, None) , ( protein: p65, None) , ( protein: NF-kappa B, None) " }

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{ "id": "388", "sentence": "The AP-1 binding proteins contained c-Jun , Jun-D , and Fra-1 , but marginal amounts of Jun-B and c-Fos .", "label": " ( protein: c-Jun, None) , ( protein: AP-1 binding proteins, None) , ( protein: c-Fos, None) , ( protein: Jun-B, None) , ( protein: Jun-D, None) , ( protein: AP-1, None) , ( protein: Fra-1, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: c-Jun, None) , ( protein: AP-1 binding proteins, None) , ( protein: c-Fos, None) , ( protein: Jun-B, None) , ( protein: Jun-D, None) , ( protein: AP-1, None) , ( protein: Fra-1, None) " }

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{ "id": "389", "sentence": "Our results indicate distinct effects of B7 and LFA-3 costimulation on the activity of AP-1 and NF-kappa B .", "label": " ( protein: AP-1, None) , ( protein: B7, None) , ( protein: LFA-3, None) , ( protein: NF-kappa B, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: AP-1, None) , ( protein: B7, None) , ( protein: LFA-3, None) , ( protein: NF-kappa B, None) " }

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{ "id": "390", "sentence": "These may partly account for the differential effects of B7 and LFA-3 costimulation on IL-2 expression .", "label": " ( protein: IL-2, None) , ( protein: B7, None) , ( protein: LFA-3, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: IL-2, None) , ( protein: B7, None) , ( protein: LFA-3, None) " }

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{ "id": "391", "sentence": "The Ah receptor recognizes DNA binding sites for the B cell transcription factor , BSAP : a possible mechanism for dioxin-mediated alteration of CD19 gene expression in human B lymphocytes .", "label": " ( DNA: CD19 gene, None) , ( cell type: human B lymphocytes, None) , ( protein: Ah receptor, None) , ( protein: transcription factor, None) , ( protein: BSAP, None) , ( DNA: DNA binding sites, None) , ( protein: B cell transcription factor, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: CD19 gene, None) , ( cell type: human B lymphocytes, None) , ( protein: Ah receptor, None) , ( protein: transcription factor, None) , ( protein: BSAP, None) , ( DNA: DNA binding sites, None) , ( protein: B cell transcription factor, None) " }

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{ "id": "392", "sentence": "2,3,7,8-tetrachlorodibenzo-p-dioxin ( TCDD ) inhibits murine and human B lymphocyte immunoglobulin production through an unknown mechanism.", "label": " None", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " None" }

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{ "id": "393", "sentence": "This study investigated the effect of TCDD on expression of the CD19 gene in a human B lymphocyte cell line .", "label": " ( DNA: CD19 gene, None) , ( cell line: human B lymphocyte cell line, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: CD19 gene, None) , ( cell line: human B lymphocyte cell line, None) " }

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{ "id": "394", "sentence": "Northern blot analysis showed that TCDD treatment decreased steady state levels of CD19 mRNA by 67% in the IM-9 cell line .", "label": " ( RNA: CD19 mRNA, None) , ( cell line: IM-9 cell line, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( RNA: CD19 mRNA, None) , ( cell line: IM-9 cell line, None) " }

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{ "id": "395", "sentence": "Using a gel mobility shift assay , we identified a DNA-binding complex in IM-9 nuclear extracts that by several criteria appears to be the Ah receptor .", "label": " ( protein: DNA-binding complex, None) , ( protein: Ah receptor, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: DNA-binding complex, None) , ( protein: Ah receptor, None) " }

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{ "id": "396", "sentence": "In addition, the Ah receptor complex recognized a DNA binding site for B cell lineage-specific activator protein ( BSAP ) in the promoter region of the human CD19 gene which is similar to the consensus Ah receptor DNA binding site .", "label": " ( DNA: DNA binding site, None) , ( protein: Ah receptor, None) , ( protein: B cell lineage-specific activator protein, None) , ( DNA: CD19 gene, None) , ( protein: Ah receptor complex, None) , ( protein: BSAP, None) , ( DNA: promoter region, None) , ( DNA: consensus Ah receptor DNA binding site, None) , ( DNA: DNA binding site, None) , ( protein: Ah receptor, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: DNA binding site, None) , ( protein: Ah receptor, None) , ( protein: B cell lineage-specific activator protein, None) , ( DNA: CD19 gene, None) , ( protein: Ah receptor complex, None) , ( protein: BSAP, None) , ( DNA: promoter region, None) , ( DNA: consensus Ah receptor DNA binding site, None) , ( DNA: DNA binding site, None) , ( protein: Ah receptor, None) " }

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{ "id": "397", "sentence": "These results suggest that the AhR could interfere with BSAP -stimulated CD19 gene transcription by competition for a common DNA binding site .", "label": " ( DNA: DNA binding site, None) , ( protein: BSAP, None) , ( DNA: CD19 gene, None) , ( protein: AhR, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: DNA binding site, None) , ( protein: BSAP, None) , ( DNA: CD19 gene, None) , ( protein: AhR, None) " }

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{ "id": "398", "sentence": "Inhibitory action of nm23 proteins on induction of erythroid differentiation of human leukemia cells .", "label": " ( cell type: human leukemia cells, None) , ( protein: nm23 proteins, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell type: human leukemia cells, None) , ( protein: nm23 proteins, None) " }

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{ "id": "399", "sentence": "We recently identified a differentiation inhibitory factor ( I-factor ) in mouse myeloid leukemia M1 cells as a murine homolog of the human nm23-H2 gene product .", "label": " ( cell line: mouse myeloid leukemia M1 cells, None) , ( protein: human nm23-H2 gene product, None) , ( protein: murine homolog, None) , ( protein: I-factor, None) , ( protein: differentiation inhibitory factor, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell line: mouse myeloid leukemia M1 cells, None) , ( protein: human nm23-H2 gene product, None) , ( protein: murine homolog, None) , ( protein: I-factor, None) , ( protein: differentiation inhibitory factor, None) " }