Datasets:

huy-nh-2000

/

instructUIE

like 0

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{ "id": "100", "sentence": "In contrast, IL-12 induction of IFN-gamma cytoplasmic mRNA appears to only partially depend on activation of protein kinase C .", "label": " ( protein: IFN-gamma, None) , ( RNA: IFN-gamma cytoplasmic mRNA, None) , ( protein: protein kinase C, None) , ( protein: IL-12, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: IFN-gamma, None) , ( RNA: IFN-gamma cytoplasmic mRNA, None) , ( protein: protein kinase C, None) , ( protein: IL-12, None) " }

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{ "id": "101", "sentence": "Furthermore, both transforming growth factor-beta and genistein , a tyrosine kinase inhibitor , could suppress IL-2 and IL-12 signaling but CsA was generally inactive.", "label": " ( protein: IL-2, None) , ( protein: IL-12, None) , ( protein: transforming growth factor-beta, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: IL-2, None) , ( protein: IL-12, None) , ( protein: transforming growth factor-beta, None) " }

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{ "id": "102", "sentence": "It also was observed that suppression of cytokine gene expression by these agents was independent of the inhibition of proliferation .", "label": " ( DNA: cytokine gene, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: cytokine gene, None) " }

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{ "id": "103", "sentence": "In addition, IL-2 but not IL-12 induced nuclear factors NF-kappa B and AP1 , and regulation of the nuclear levels of these two DNA binding protein complexes is correlated with IFN-gamma and GM-CSF gene expression .", "label": " ( protein: IL-2, None) , ( protein: AP1, None) , ( protein: nuclear factors, None) , ( protein: NF-kappa B, None) , ( protein: IL-12, None) , ( protein: DNA binding protein complexes, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: IL-2, None) , ( protein: AP1, None) , ( protein: nuclear factors, None) , ( protein: NF-kappa B, None) , ( protein: IL-12, None) , ( protein: DNA binding protein complexes, None) " }

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{ "id": "104", "sentence": "These data indicate that IL-2 and IL-12 may have distinct signaling pathways leading to the induction of IFN-gamma and GM-CSF gene expression , and that the NK3.3 cell line may serve as a novel model for dissecting the biochemical and molecular events involved in these pathways.", "label": " ( cell line: NK3.3 cell line, None) , ( protein: IL-2, None) , ( protein: IL-12, None) , ( protein: IFN-gamma, None) , ( protein: GM-CSF, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell line: NK3.3 cell line, None) , ( protein: IL-2, None) , ( protein: IL-12, None) , ( protein: IFN-gamma, None) , ( protein: GM-CSF, None) " }

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{ "id": "105", "sentence": "A functional T-cell receptor signaling pathway is required for p95vav activity .", "label": " ( protein: T-cell receptor, None) , ( protein: p95vav, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: T-cell receptor, None) , ( protein: p95vav, None) " }

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{ "id": "106", "sentence": "Stimulation of the T-cell antigen receptor ( TCR ) induces activation of multiple tyrosine kinases , resulting in phosphorylation of numerous intracellular substrates .", "label": " ( protein: TCR, None) , ( protein: tyrosine kinases, None) , ( protein: T-cell antigen receptor, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: TCR, None) , ( protein: tyrosine kinases, None) , ( protein: T-cell antigen receptor, None) " }

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{ "id": "107", "sentence": "One substrate is p95vav , which is expressed exclusively in hematopoietic and trophoblast cells .", "label": " ( protein: p95vav, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: p95vav, None) " }

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{ "id": "108", "sentence": "It contains a number of structural motifs , including Src homology 2 , Src homology 3 , and pleckstrin homology domains and a putative guanine nucleotide exchange domain .", "label": " ( protein: structural motifs, None) , ( protein: Src homology 2 , Src homology 3 , and pleckstrin homology domains, None) , ( protein: Src homology 2, None) , ( protein: pleckstrin homology domains, None) , ( protein: Src homology 3, None) , ( protein: putative guanine nucleotide exchange domain, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: structural motifs, None) , ( protein: Src homology 2 , Src homology 3 , and pleckstrin homology domains, None) , ( protein: Src homology 2, None) , ( protein: pleckstrin homology domains, None) , ( protein: Src homology 3, None) , ( protein: putative guanine nucleotide exchange domain, None) " }

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{ "id": "109", "sentence": "The role of p95vav in TCR -mediated signaling processes is unclear.", "label": " ( protein: p95vav, None) , ( protein: TCR, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: p95vav, None) , ( protein: TCR, None) " }

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{ "id": "110", "sentence": "Here, we show that overexpression of p95vav alone in Jurkat T cells leads to activation of the nuclear factors , including NFAT , involved in interleukin-2 expression .", "label": " ( protein: interleukin-2, None) , ( protein: nuclear factors, None) , ( protein: NFAT, None) , ( cell type: T cells, None) , ( protein: p95vav, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: interleukin-2, None) , ( protein: nuclear factors, None) , ( protein: NFAT, None) , ( cell type: T cells, None) , ( protein: p95vav, None) " }

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{ "id": "111", "sentence": "Furthermore, p95vav synergizes with TCR stimulation in inducing NFAT- and interleukin-2- dependent transcription .", "label": " ( protein: p95vav, None) , ( protein: TCR, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: p95vav, None) , ( protein: TCR, None) " }

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{ "id": "112", "sentence": "In contrast, NFAT activation by a G-protein-coupled receptor is not modulated by p95vav overexpression , suggesting that the effect is specific to the TCR signaling pathways .", "label": " ( protein: TCR, None) , ( protein: NFAT, None) , ( protein: p95vav, None) , ( protein: G-protein-coupled receptor, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: TCR, None) , ( protein: NFAT, None) , ( protein: p95vav, None) , ( protein: G-protein-coupled receptor, None) " }

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{ "id": "113", "sentence": "Although removal of the first 67 amino acids of p95vav activates its transforming potential in NIH 3T3 cells , this region appears to be required for its function in T cells .", "label": " ( cell type: T cells, None) , ( protein: p95vav, None) , ( cell line: NIH 3T3 cells, None) , ( protein: first 67 amino acids, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell type: T cells, None) , ( protein: p95vav, None) , ( cell line: NIH 3T3 cells, None) , ( protein: first 67 amino acids, None) " }

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{ "id": "114", "sentence": "We further demonstrate that the p95vav -induced NFAT activation is not mimicked by Ras activation , though its function is dependent upon Ras and Raf .", "label": " ( protein: p95vav, None) , ( protein: NFAT, None) , ( protein: Raf, None) , ( protein: Ras, None) , ( protein: Ras, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: p95vav, None) , ( protein: NFAT, None) , ( protein: Raf, None) , ( protein: Ras, None) , ( protein: Ras, None) " }

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{ "id": "115", "sentence": "Furthermore, the activating function of p95vav is blocked by FK506 , suggesting that its activity also depends on calcineurin .", "label": " ( protein: calcineurin, None) , ( protein: p95vav, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: calcineurin, None) , ( protein: p95vav, None) " }

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{ "id": "116", "sentence": "To further dissect p95vav involvement in TCR signaling , we analyzed various Jurkat mutants deficient in TCR signaling function or TCR expression and showed that an intact TCR signaling pathway is required for p95vav to function.", "label": " ( protein: TCR, None) , ( cell line: Jurkat mutants, None) , ( protein: TCR, None) , ( protein: p95vav, None) , ( protein: TCR, None) , ( protein: p95vav, None) , ( protein: TCR, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: TCR, None) , ( cell line: Jurkat mutants, None) , ( protein: TCR, None) , ( protein: p95vav, None) , ( protein: TCR, None) , ( protein: p95vav, None) , ( protein: TCR, None) " }

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{ "id": "117", "sentence": "However, overexpression of p95vav does not appear to influence TCR -induced protein tyrosine phosphorylation or increases in cytoplasmic free calcium .", "label": " ( protein: p95vav, None) , ( protein: TCR, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: p95vav, None) , ( protein: TCR, None) " }

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{ "id": "118", "sentence": "Taken together, our data suggest that p95vav plays an important role at an yet unidentified proximal position in the TCR signaling cascade .", "label": " ( protein: TCR, None) , ( protein: p95vav, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: TCR, None) , ( protein: p95vav, None) " }

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{ "id": "119", "sentence": "Positive and negative regulation of granulocyte-macrophage colony-stimulating factor promoter activity by AML1-related transcription factor , PEBP2 .", "label": " ( protein: PEBP2, None) , ( protein: AML1-related transcription factor, None) , ( protein: granulocyte-macrophage colony-stimulating factor, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: PEBP2, None) , ( protein: AML1-related transcription factor, None) , ( protein: granulocyte-macrophage colony-stimulating factor, None) " }

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{ "id": "120", "sentence": "The granulocyte-macrophage colony-stimulating factor ( GM-CSF ) gene promoter contains a consensus sequence for the polyomavirus enhancer binding-protein 2 ( PEBP2 ) transcription factor , which consists of alpha and beta subunits .", "label": " ( protein: polyomavirus enhancer binding-protein 2 ( PEBP2 ) transcription factor, None) , ( protein: polyomavirus enhancer binding-protein 2, None) , ( protein: PEBP2, None) , ( DNA: granulocyte-macrophage colony-stimulating factor ( GM-CSF ) gene promoter, None) , ( protein: GM-CSF, None) , ( protein: granulocyte-macrophage colony-stimulating factor, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: polyomavirus enhancer binding-protein 2 ( PEBP2 ) transcription factor, None) , ( protein: polyomavirus enhancer binding-protein 2, None) , ( protein: PEBP2, None) , ( DNA: granulocyte-macrophage colony-stimulating factor ( GM-CSF ) gene promoter, None) , ( protein: GM-CSF, None) , ( protein: granulocyte-macrophage colony-stimulating factor, None) " }

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{ "id": "121", "sentence": "There are at least two genes, alpha A and alpha B , encoding the alpha subunit .", "label": " ( DNA: alpha A, None) , ( DNA: alpha B, None) , ( protein: alpha subunit, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: alpha A, None) , ( DNA: alpha B, None) , ( protein: alpha subunit, None) " }

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{ "id": "122", "sentence": "alpha B is the mouse homologue of human AML1 gene detected at the breakpoints of t(8;21) and t(3;21) myeloid leukemias .", "label": " ( DNA: human AML1 gene, None) , ( DNA: mouse homologue, None) , ( DNA: alpha B, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: human AML1 gene, None) , ( DNA: mouse homologue, None) , ( DNA: alpha B, None) " }

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{ "id": "123", "sentence": "We examined alpha A1 (an alpha A -gene product ) and alpha B1 and alpha B2 (two alpha B -encoded isomers ) for their effects on the GM-CSF promoter .", "label": " ( protein: alpha B1, None) , ( DNA: alpha A, None) , ( protein: alpha B, None) , ( protein: alpha B2, None) , ( DNA: GM-CSF promoter, None) , ( protein: alpha A1, None) , ( protein: alpha A -gene product, None) , ( protein: alpha B -encoded isomers, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: alpha B1, None) , ( DNA: alpha A, None) , ( protein: alpha B, None) , ( protein: alpha B2, None) , ( DNA: GM-CSF promoter, None) , ( protein: alpha A1, None) , ( protein: alpha A -gene product, None) , ( protein: alpha B -encoded isomers, None) " }

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{ "id": "124", "sentence": "PEBP2 alpha A1 , alpha B1 , and alpha B2 proteins bound the PEBP2 site within the mouse GM-CSF promoter .", "label": " ( protein: alpha B2 proteins, None) , ( protein: PEBP2, None) , ( protein: alpha B1, None) , ( protein: PEBP2, None) , ( protein: alpha A1, None) , ( DNA: PEBP2 site, None) , ( protein: PEBP2 alpha A1, None) , ( DNA: mouse GM-CSF promoter, None) , ( protein: GM-CSF, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: alpha B2 proteins, None) , ( protein: PEBP2, None) , ( protein: alpha B1, None) , ( protein: PEBP2, None) , ( protein: alpha A1, None) , ( DNA: PEBP2 site, None) , ( protein: PEBP2 alpha A1, None) , ( DNA: mouse GM-CSF promoter, None) , ( protein: GM-CSF, None) " }

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{ "id": "125", "sentence": "PEBP2 alpha A1 and alpha B1 enhanced the expression of the GM-CSF promoter-driven reporter plasmid in unstimulated and 12-O-tetradecanoylphorbol 13-acetate/phytohemagglutinin-stimulated human Jurkat T cells .", "label": " ( protein: GM-CSF, None) , ( protein: alpha B1, None) , ( protein: alpha A1, None) , ( DNA: GM-CSF promoter-driven reporter plasmid, None) , ( protein: PEBP2, None) , ( protein: PEBP2 alpha A1, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: GM-CSF, None) , ( protein: alpha B1, None) , ( protein: alpha A1, None) , ( DNA: GM-CSF promoter-driven reporter plasmid, None) , ( protein: PEBP2, None) , ( protein: PEBP2 alpha A1, None) " }

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{ "id": "126", "sentence": "In contrast, the promoter activity was suppressed by alpha B2 .", "label": " ( protein: alpha B2, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: alpha B2, None) " }

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{ "id": "127", "sentence": "Coexpression of alpha B1 and alpha B2 showed that the promoter activity could be determined by the alpha B1 /alpha B2 ratio .", "label": " ( protein: alpha B1, None) , ( protein: alpha B1, None) , ( protein: /alpha B2, None) , ( protein: alpha B2, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: alpha B1, None) , ( protein: alpha B1, None) , ( protein: /alpha B2, None) , ( protein: alpha B2, None) " }

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{ "id": "128", "sentence": "Jurkat cell extract contained PEBP2 site-binding protein (s) that cross-reacted with antimouse alpha A1 antibodies .", "label": " ( protein: antimouse alpha A1 antibodies, None) , ( protein: PEBP2 site-binding protein, None) , ( protein: alpha A1, None) , ( protein: PEBP2, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: antimouse alpha A1 antibodies, None) , ( protein: PEBP2 site-binding protein, None) , ( protein: alpha A1, None) , ( protein: PEBP2, None) " }

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{ "id": "129", "sentence": "Northern blot analysis indicated the expression of human PEBP2 alpha A , alpha B ( AML1 ), and beta genes in Jurkat cells .", "label": " ( DNA: beta genes, None) , ( cell line: Jurkat cells, None) , ( DNA: AML1, None) , ( protein: PEBP2 alpha A, None) , ( protein: PEBP2, None) , ( protein: alpha B, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: beta genes, None) , ( cell line: Jurkat cells, None) , ( DNA: AML1, None) , ( protein: PEBP2 alpha A, None) , ( protein: PEBP2, None) , ( protein: alpha B, None) " }

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{ "id": "130", "sentence": "Although further studies are required to determine the precise role of PEBP2 in the GM-CSF promoter activity , the present findings suggested the importance of the relative ratio of different PEBP2 isoforms in regulating the levels of the promoter activity .", "label": " ( protein: PEBP2, None) , ( protein: PEBP2, None) , ( protein: GM-CSF, None) , ( protein: PEBP2 isoforms, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: PEBP2, None) , ( protein: PEBP2, None) , ( protein: GM-CSF, None) , ( protein: PEBP2 isoforms, None) " }

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{ "id": "131", "sentence": "IFN-gamma priming of monocytes enhances LPS -induced TNF production by augmenting both transcription and MRNA stability .", "label": " ( protein: TNF, None) , ( cell type: monocytes, None) , ( protein: IFN-gamma, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: TNF, None) , ( cell type: monocytes, None) , ( protein: IFN-gamma, None) " }

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{ "id": "132", "sentence": "The induction of cytokine expression in monocytes / macrophages by bacterial endotoxin or lipopolysaccharide is a critical, highly regulated host defence response .", "label": " ( protein: cytokine, None) , ( cell type: monocytes, None) , ( protein: bacterial endotoxin, None) , ( cell type: macrophages, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: cytokine, None) , ( cell type: monocytes, None) , ( protein: bacterial endotoxin, None) , ( cell type: macrophages, None) " }

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{ "id": "133", "sentence": "The augmentation of LPS responses by interferon gamma ( IFN-gamma ), referred to as priming, is well established.", "label": " ( protein: IFN-gamma, None) , ( protein: interferon gamma, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: IFN-gamma, None) , ( protein: interferon gamma, None) " }

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{ "id": "134", "sentence": "However, the mechanism(s) by which priming occurs is poorly defined.", "label": " None", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " None" }

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{ "id": "135", "sentence": "Using tumour necrosis factor ( TNF ) induction as a model, experiments were designed to analyse in detail the priming effect on the LPS response in human monocytes .", "label": " ( cell type: human monocytes, None) , ( protein: tumour necrosis factor, None) , ( protein: TNF, None) , ( cell type: monocytes, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell type: human monocytes, None) , ( protein: tumour necrosis factor, None) , ( protein: TNF, None) , ( cell type: monocytes, None) " }

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{ "id": "136", "sentence": "Priming by IFN-gamma was primarily manifested at the level of TNF mRNA accumulation .", "label": " ( RNA: TNF mRNA, None) , ( protein: IFN-gamma, None) , ( protein: TNF, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( RNA: TNF mRNA, None) , ( protein: IFN-gamma, None) , ( protein: TNF, None) " }

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{ "id": "137", "sentence": "IFN-gamma pre-treatment affected the magnitude rather than the sensitivity of the LPS response .", "label": " ( protein: IFN-gamma, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: IFN-gamma, None) " }

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{ "id": "138", "sentence": "Priming occurred after several hours of treatment , and the primed state was induced by either IFN-gamma or GM-CSF , but not M-CSF .", "label": " ( protein: IFN-gamma, None) , ( protein: M-CSF, None) , ( protein: GM-CSF, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: IFN-gamma, None) , ( protein: M-CSF, None) , ( protein: GM-CSF, None) " }

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{ "id": "139", "sentence": "Primed monocytes transcribed TNF mRNA at a higher rate than freshly isolated monocytes upon activation with LPS .", "label": " ( RNA: TNF mRNA, None) , ( cell type: monocytes, None) , ( protein: TNF, None) , ( cell type: monocytes, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( RNA: TNF mRNA, None) , ( cell type: monocytes, None) , ( protein: TNF, None) , ( cell type: monocytes, None) " }

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{ "id": "140", "sentence": "The increased transcriptional rate correlated with a marked increase in nuclear factor-kappa B activity in these cells as determined by electrophoretic mobility shift assay using a consensus NF-kappa B oligonucleotide .", "label": " ( protein: nuclear factor-kappa B, None) , ( DNA: consensus NF-kappa B oligonucleotide, None) , ( protein: NF-kappa B, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: nuclear factor-kappa B, None) , ( DNA: consensus NF-kappa B oligonucleotide, None) , ( protein: NF-kappa B, None) " }

[]

{ "id": "141", "sentence": "An additional significant finding was than TNF mRNA induced in primed cells was much more stable than in unprimed cells (T1/2 increased 6-8-fold).", "label": " ( cell line: primed cells, None) , ( RNA: TNF mRNA, None) , ( protein: TNF, None) , ( cell line: unprimed cells, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell line: primed cells, None) , ( RNA: TNF mRNA, None) , ( protein: TNF, None) , ( cell line: unprimed cells, None) " }

[]

{ "id": "142", "sentence": "Consistent with the increased mRNA stability , the duration of mRNA accumulation was longer following LPS stimulation in primed monocytes , in addition to being of greater magnitude.", "label": " ( cell type: monocytes, None) , ( RNA: mRNA, None) , ( RNA: mRNA, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell type: monocytes, None) , ( RNA: mRNA, None) , ( RNA: mRNA, None) " }

[]

{ "id": "143", "sentence": "Finally, primed and unprimed cells possessed a differential sensitivity to the kinase inhibitor H-89 .", "label": " None", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " None" }

[]

{ "id": "144", "sentence": "H-89 substantially suppressed LPS -induced TNF mRNA accumulation in unprimed cells , but had no effect on primed monocytes following LPS stimulation .", "label": " ( cell line: primed monocytes, None) , ( protein: TNF, None) , ( cell type: monocytes, None) , ( cell line: unprimed cells, None) , ( RNA: TNF mRNA, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell line: primed monocytes, None) , ( protein: TNF, None) , ( cell type: monocytes, None) , ( cell line: unprimed cells, None) , ( RNA: TNF mRNA, None) " }

[]

{ "id": "145", "sentence": "(ABSTRACT TRUNCATED AT 250 WORDS)", "label": " None", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " None" }

[]

{ "id": "146", "sentence": "A Myc-associated zinc finger protein binding site is one of four important functional regions in the CD4 promoter .", "label": " ( protein: CD4, None) , ( DNA: Myc-associated zinc finger protein binding site, None) , ( DNA: CD4 promoter, None) , ( DNA: functional regions, None) , ( protein: Myc-associated zinc finger protein, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: CD4, None) , ( DNA: Myc-associated zinc finger protein binding site, None) , ( DNA: CD4 promoter, None) , ( DNA: functional regions, None) , ( protein: Myc-associated zinc finger protein, None) " }

[]

{ "id": "147", "sentence": "The CD4 promoter plays an important role in the developmental control of CD4 transcription .", "label": " ( protein: CD4, None) , ( DNA: CD4 promoter, None) , ( protein: CD4, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: CD4, None) , ( DNA: CD4 promoter, None) , ( protein: CD4, None) " }

[]

{ "id": "148", "sentence": "In this report, we show that the minimal CD4 promoter has four factor binding sites , each of which is required for full function.", "label": " ( DNA: CD4 promoter, None) , ( DNA: factor binding sites, None) , ( protein: CD4, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: CD4 promoter, None) , ( DNA: factor binding sites, None) , ( protein: CD4, None) " }

[]

{ "id": "149", "sentence": "Using biochemical and mutagenesis analyses , we determined that multiple nuclear factors bind to these independent sites.", "label": " ( protein: nuclear factors, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: nuclear factors, None) " }

[]

{ "id": "150", "sentence": "We determined that an initiator-like sequence present at the cap site and an Ets consensus sequence are required for full promoter function .", "label": " ( DNA: Ets consensus sequence, None) , ( DNA: cap site, None) , ( DNA: initiator-like sequence, None) , ( DNA: promoter, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: Ets consensus sequence, None) , ( DNA: cap site, None) , ( DNA: initiator-like sequence, None) , ( DNA: promoter, None) " }

[]

{ "id": "151", "sentence": "We also demonstrate that the Myc-associated zinc finger protein ( MAZ ) appears to be the predominant factor binding to one of these sites.", "label": " ( protein: zinc finger, None) , ( protein: MAZ, None) , ( protein: predominant factor, None) , ( protein: Myc-associated zinc finger protein, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: zinc finger, None) , ( protein: MAZ, None) , ( protein: predominant factor, None) , ( protein: Myc-associated zinc finger protein, None) " }

[]

{ "id": "152", "sentence": "This last site closely resembles the ME1a1 G3AG4AG3 motif previously shown to be a critical element in the P2 promoter of the c-myc gene .", "label": " ( DNA: ME1a1 G3AG4AG3 motif, None) , ( DNA: c-myc gene, None) , ( DNA: P2 promoter, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: ME1a1 G3AG4AG3 motif, None) , ( DNA: c-myc gene, None) , ( DNA: P2 promoter, None) " }

[]

{ "id": "153", "sentence": "We therefore believe that the MAZ transcription factor is also likely to play an important role in the control of developmental expression of the CD4 gene .", "label": " ( protein: CD4, None) , ( protein: MAZ transcription factor, None) , ( protein: MAZ, None) , ( DNA: CD4 gene, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: CD4, None) , ( protein: MAZ transcription factor, None) , ( protein: MAZ, None) , ( DNA: CD4 gene, None) " }

[]

{ "id": "154", "sentence": "Erythropoietin stimulates transcription of the TAL1/SCL gene and phosphorylation of its protein products .", "label": " ( DNA: TAL1/SCL gene, None) , ( protein: Erythropoietin, None) , ( protein: protein products, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: TAL1/SCL gene, None) , ( protein: Erythropoietin, None) , ( protein: protein products, None) " }

[]

{ "id": "155", "sentence": "Activation of the TAL1 (or SCL) gene , originally identified through its involvement by a recurrent chromosomal translocation , is the most frequent molecular lesion recognized in T-cell acute lymphoblastic leukemia .", "label": " ( DNA: TAL1 (or SCL) gene, None) , ( DNA: molecular lesion, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: TAL1 (or SCL) gene, None) , ( DNA: molecular lesion, None) " }

[]

{ "id": "156", "sentence": "The protein products of this gene contain the basic-helix-loop-helix motif characteristic of a large family of transcription factors that bind to the canonical DNA sequence CANNTG as protein heterodimers .", "label": " ( DNA: canonical DNA sequence CANNTG, None) , ( protein: basic-helix-loop-helix motif, None) , ( protein: protein products, None) , ( protein: protein heterodimers, None) , ( protein: transcription factors, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: canonical DNA sequence CANNTG, None) , ( protein: basic-helix-loop-helix motif, None) , ( protein: protein products, None) , ( protein: protein heterodimers, None) , ( protein: transcription factors, None) " }

[]

{ "id": "157", "sentence": "TAL1 expression by erythroid cells in vivo and in chemical-induced erythroleukemia cell lines in vivo suggested the gene might regulate aspects of erythroid differentiation .", "label": " ( protein: TAL1, None) , ( cell type: erythroid cells, None) , ( cell line: chemical-induced erythroleukemia cell lines, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: TAL1, None) , ( cell type: erythroid cells, None) , ( cell line: chemical-induced erythroleukemia cell lines, None) " }

[]

{ "id": "158", "sentence": "Since the terminal events of erythropoiesis are controlled by the glycoprotein hormone erythropoietin ( Epo ), we investigated whether the expression or activity of the TAL1 gene and its protein products were affected by Epo in splenic erythroblasts from mice infected with an anemia-inducing strain of Friend virus (FVA cells).", "label": " ( protein: Epo, None) , ( DNA: TAL1 gene, None) , ( protein: Epo, None) , ( protein: glycoprotein hormone erythropoietin, None) , ( protein: TAL1, None) , ( cell type: splenic erythroblasts, None) , ( protein: protein products, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: Epo, None) , ( DNA: TAL1 gene, None) , ( protein: Epo, None) , ( protein: glycoprotein hormone erythropoietin, None) , ( protein: TAL1, None) , ( cell type: splenic erythroblasts, None) , ( protein: protein products, None) " }

[]

{ "id": "159", "sentence": "Epo elicited a rapid, dose-related increase in TAL1 mRNA by increasing transcription of the gene and stabilizing one of its mRNAs .", "label": " ( RNA: TAL1 mRNA, None) , ( RNA: mRNAs, None) , ( protein: Epo, None) , ( protein: TAL1, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( RNA: TAL1 mRNA, None) , ( RNA: mRNAs, None) , ( protein: Epo, None) , ( protein: TAL1, None) " }

[]

{ "id": "160", "sentence": "An Epo -inducible TAL1 DNA binding activity was identified in FVA cell nuclear extracts that subsequently decayed despite accumulating mRNA and protein .", "label": " ( RNA: mRNA, None) , ( protein: TAL1, None) , ( protein: protein, None) , ( protein: Epo, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( RNA: mRNA, None) , ( protein: TAL1, None) , ( protein: protein, None) , ( protein: Epo, None) " }

[]

{ "id": "161", "sentence": "Induction of DNA binding activity was associated temporally with Epo -induced phosphorylation of nuclear TAL1 protein .", "label": " ( protein: nuclear TAL1 protein, None) , ( protein: Epo, None) , ( protein: TAL1, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: nuclear TAL1 protein, None) , ( protein: Epo, None) , ( protein: TAL1, None) " }

[]

{ "id": "162", "sentence": "These results indicate that Epo acts at both transcriptional and posttranscriptional levels on the TAL1 locus in Friend virus -induced erythroblasts and establish a link between Epo signaling mechanisms and a member of a family of transcription factors involved in the differentiation of diverse cell lineages .", "label": " ( cell line: Friend virus -induced erythroblasts, None) , ( protein: Epo, None) , ( protein: TAL1, None) , ( cell type: diverse cell lineages, None) , ( protein: transcription factors, None) , ( protein: Epo, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell line: Friend virus -induced erythroblasts, None) , ( protein: Epo, None) , ( protein: TAL1, None) , ( cell type: diverse cell lineages, None) , ( protein: transcription factors, None) , ( protein: Epo, None) " }

[]

{ "id": "163", "sentence": "Nonradioactive quantification of glucocorticoid receptor expression during differentiation of human monocytic cells ( U937 ).", "label": " ( cell type: monocytic cells, None) , ( cell line: U937, None) , ( protein: glucocorticoid receptor, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell type: monocytic cells, None) , ( cell line: U937, None) , ( protein: glucocorticoid receptor, None) " }

[]

{ "id": "164", "sentence": "We describe a method for relative quantification of specific mRNA using a nonradioactive assay based on DNA strand competition between identical sequences of biotin- and fluorescein-labeled amplicon (probe) and unlabeled amplicon (target) during hybridization .", "label": " ( RNA: mRNA, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( RNA: mRNA, None) " }

[]

{ "id": "165", "sentence": "As the target quantity increased, that of the double-labeled probe decreased in accordance with the mass action law.", "label": " None", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " None" }

[]

{ "id": "166", "sentence": "This technique was successfully applied to evaluate differences in glucocorticoid receptor expression in U937 cells before and after the addition of potent differentiation inducers : 12-O-tetradecanoylphorbol 13-acetate ( TPA ) and a combination of all-trans retinoic acid ( RA ) and 1,25-dihydroxyvitamin D2 ( VD ).", "label": " ( cell line: U937 cells, None) , ( protein: glucocorticoid receptor, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell line: U937 cells, None) , ( protein: glucocorticoid receptor, None) " }

[]

{ "id": "167", "sentence": "We observed that TPA treatment was associated with an increase in specific binding of [3H]dexamethasone and up-regulation of GR mRNA while no enhanced GR expression was perceived with RA/VD treatment .", "label": " ( protein: GR, None) , ( protein: GR, None) , ( RNA: GR mRNA, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: GR, None) , ( protein: GR, None) , ( RNA: GR mRNA, None) " }

[]

{ "id": "168", "sentence": "Induction of Sp1 phosphorylation and NF-kappa B -independent HIV promoter domain activity in T lymphocytes stimulated by okadaic acid .", "label": " ( DNA: NF-kappa B -independent HIV promoter, None) , ( protein: NF-kappa B, None) , ( protein: Sp1, None) , ( cell type: T lymphocytes, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: NF-kappa B -independent HIV promoter, None) , ( protein: NF-kappa B, None) , ( protein: Sp1, None) , ( cell type: T lymphocytes, None) " }

[]

{ "id": "169", "sentence": "In contrast to the purely enhancer-dependent effect of cytokines such as TNF on the activity of the HIV regulatory region ( LTR ), we observed that okadaic acid ( OKA ) activates HIV transcription through both the enhancer, responding to the factor NF-kappa B , and the promoter domain of the LTR .", "label": " ( DNA: LTR, None) , ( DNA: HIV regulatory region, None) , ( protein: NF-kappa B, None) , ( protein: cytokines, None) , ( DNA: LTR, None) , ( DNA: promoter domain, None) , ( protein: TNF, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: LTR, None) , ( DNA: HIV regulatory region, None) , ( protein: NF-kappa B, None) , ( protein: cytokines, None) , ( DNA: LTR, None) , ( DNA: promoter domain, None) , ( protein: TNF, None) " }

[]

{ "id": "170", "sentence": "The inducibility of HIV LTR -driven luciferase expression constructs in lymphoblastoid cells stimulated by OKA depended on both functional Sp1 binding elements and the ability of the TATA box to bind the protein TBP .", "label": " ( protein: Sp1, None) , ( DNA: HIV LTR, None) , ( DNA: TATA box, None) , ( DNA: HIV LTR -driven luciferase expression constructs, None) , ( protein: luciferase, None) , ( cell type: lymphoblastoid cells, None) , ( protein: TBP, None) , ( DNA: functional Sp1 binding elements, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: Sp1, None) , ( DNA: HIV LTR, None) , ( DNA: TATA box, None) , ( DNA: HIV LTR -driven luciferase expression constructs, None) , ( protein: luciferase, None) , ( cell type: lymphoblastoid cells, None) , ( protein: TBP, None) , ( DNA: functional Sp1 binding elements, None) " }

[]

{ "id": "171", "sentence": "In both transformed and normal lymphocytes , OKA stimulation induced intense phosphorylation of the constitutively expressed Sp1 protein in the nucleus , a property of OKA not shared by TNF , phorbol ester , or PHA and interleukin 2 .", "label": " ( protein: TNF, None) , ( protein: Sp1, None) , ( protein: Sp1 protein, None) , ( protein: interleukin 2, None) , ( protein: PHA, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: TNF, None) , ( protein: Sp1, None) , ( protein: Sp1 protein, None) , ( protein: interleukin 2, None) , ( protein: PHA, None) " }

[]

{ "id": "172", "sentence": "Responsiveness of LTR constructs deleted of kappa B elements to HIV Tat expression was increased upon OKA but not TNF stimulation .", "label": " ( DNA: LTR constructs, None) , ( protein: HIV Tat, None) , ( protein: TNF, None) , ( DNA: kappa B elements, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: LTR constructs, None) , ( protein: HIV Tat, None) , ( protein: TNF, None) , ( DNA: kappa B elements, None) " }

[]

{ "id": "173", "sentence": "Our results suggest that SP1 phosphorylation induced by OKA , a selective inhibitor of the serine-threonine phosphatase PP2A , facilitates the formation of a transcription complex involving general transcription factors , HIV Tat , and Sp1 proteins .", "label": " ( protein: Sp1, None) , ( protein: transcription factors, None) , ( protein: PP2A, None) , ( protein: HIV Tat, None) , ( protein: serine-threonine phosphatase, None) , ( protein: Sp1 proteins, None) , ( protein: SP1, None) , ( protein: transcription complex, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: Sp1, None) , ( protein: transcription factors, None) , ( protein: PP2A, None) , ( protein: HIV Tat, None) , ( protein: serine-threonine phosphatase, None) , ( protein: Sp1 proteins, None) , ( protein: SP1, None) , ( protein: transcription complex, None) " }

[]

{ "id": "174", "sentence": "The formation of this complex would increase, independently of an in synergy with NF-kappa B , the low basal activity of the HIV LTR observed in normal T lymphocytes .", "label": " ( cell type: T lymphocytes, None) , ( cell type: normal T lymphocytes, None) , ( DNA: HIV LTR, None) , ( protein: NF-kappa B, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell type: T lymphocytes, None) , ( cell type: normal T lymphocytes, None) , ( DNA: HIV LTR, None) , ( protein: NF-kappa B, None) " }

[]

{ "id": "175", "sentence": "The role of shared receptor motifs and common Stat proteins in the generation of cytokine pleiotropy and redundancy by IL-2 , IL-4 , IL-7 , IL-13 , and IL-15 .", "label": " ( protein: IL-4, None) , ( protein: common Stat proteins, None) , ( protein: IL-2, None) , ( protein: cytokine, None) , ( protein: IL-13, None) , ( protein: shared receptor motifs, None) , ( protein: IL-15, None) , ( protein: IL-7, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: IL-4, None) , ( protein: common Stat proteins, None) , ( protein: IL-2, None) , ( protein: cytokine, None) , ( protein: IL-13, None) , ( protein: shared receptor motifs, None) , ( protein: IL-15, None) , ( protein: IL-7, None) " }

[]

{ "id": "176", "sentence": "To understand the molecular bases for cytokine redundancy and pleiotropy , we have compared the Stat proteins activated in peripheral blood lymphocytes ( PBLs ) by cytokines with shared and distinct actions.", "label": " ( cell type: peripheral blood lymphocytes, None) , ( cell type: PBLs, None) , ( protein: cytokines, None) , ( protein: Stat proteins, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell type: peripheral blood lymphocytes, None) , ( cell type: PBLs, None) , ( protein: cytokines, None) , ( protein: Stat proteins, None) " }

[]

{ "id": "177", "sentence": "Interleukin-2 ( IL-2 ) rapidly activated Stat5 in fresh PBL , and Stat3 and Stat5 in preactivated PBL .", "label": " ( protein: Stat5, None) , ( cell type: PBL, None) , ( protein: Stat5, None) , ( protein: Interleukin-2, None) , ( protein: IL-2, None) , ( cell type: preactivated PBL, None) , ( protein: Stat3, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: Stat5, None) , ( cell type: PBL, None) , ( protein: Stat5, None) , ( protein: Interleukin-2, None) , ( protein: IL-2, None) , ( cell type: preactivated PBL, None) , ( protein: Stat3, None) " }

[]

{ "id": "178", "sentence": "IL-7 and IL-15 induced the same complexes as IL-2 , a feature explained by the existence of similar tyrosine -phosphorylated motifs in the cytoplasmic domains of IL-2R beta and IL-7R that can serve as docking sites for Stat proteins .", "label": " ( protein: Stat proteins, None) , ( protein: cytoplasmic domains, None) , ( protein: IL-2, None) , ( protein: IL-15, None) , ( protein: IL-2R beta, None) , ( protein: tyrosine -phosphorylated motifs, None) , ( protein: IL-7R, None) , ( protein: IL-7, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: Stat proteins, None) , ( protein: cytoplasmic domains, None) , ( protein: IL-2, None) , ( protein: IL-15, None) , ( protein: IL-2R beta, None) , ( protein: tyrosine -phosphorylated motifs, None) , ( protein: IL-7R, None) , ( protein: IL-7, None) " }

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{ "id": "179", "sentence": "IL-13 Induced the same complexes as IL-4 , a finding explained by our studies implicating IL-4R as a shared component of the receptors .", "label": " ( protein: IL-4, None) , ( protein: receptors, None) , ( protein: IL-13, None) , ( protein: IL-4R, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: IL-4, None) , ( protein: receptors, None) , ( protein: IL-13, None) , ( protein: IL-4R, None) " }

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{ "id": "180", "sentence": "These studies demonstrate that a single cytokine can activate different combinations of Stat proteins under different physiological conditions , and also indicate two mechanisms by which distinct cytokines can activate the same Stat protein .", "label": " ( protein: cytokine, None) , ( protein: cytokines, None) , ( protein: Stat protein, None) , ( protein: Stat proteins, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: cytokine, None) , ( protein: cytokines, None) , ( protein: Stat protein, None) , ( protein: Stat proteins, None) " }

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{ "id": "181", "sentence": "Control of I kappa B-alpha proteolysis by site-specific, signal-induced phosphorylation .", "label": " ( protein: I kappa B-alpha, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: I kappa B-alpha, None) " }

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{ "id": "182", "sentence": "I kappa B-alpha inhibits transcription factor NF-kappa B by retaining it in the cytoplasm .", "label": " ( protein: NF-kappa B, None) , ( protein: transcription factor, None) , ( protein: I kappa B-alpha, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: NF-kappa B, None) , ( protein: transcription factor, None) , ( protein: I kappa B-alpha, None) " }

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{ "id": "183", "sentence": "Various stimuli, typically those associated with stress or pathogens, rapidly inactivate I kappa B-alpha .", "label": " ( protein: I kappa B-alpha, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: I kappa B-alpha, None) " }

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{ "id": "184", "sentence": "This liberates NF-kappa B to translocate to the nucleus and initiate transcription of genes important for the defense of the organism.", "label": " ( DNA: genes, None) , ( protein: NF-kappa B, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: genes, None) , ( protein: NF-kappa B, None) " }

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{ "id": "185", "sentence": "Activation of NF-kappa B correlates with phosphorylation of I kappa B-alpha and requires the proteolysis of this inhibitor.", "label": " ( protein: NF-kappa B, None) , ( protein: I kappa B-alpha, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: NF-kappa B, None) , ( protein: I kappa B-alpha, None) " }

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{ "id": "186", "sentence": "When either serine-32 or serine-36 of I kappa B-alpha was mutated, the protein did not undergo signal-induced phosphorylation or degradation , and NF-kappa B could not be activated.", "label": " ( protein: NF-kappa B, None) , ( protein: I kappa B-alpha, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: NF-kappa B, None) , ( protein: I kappa B-alpha, None) " }

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{ "id": "187", "sentence": "These results suggest that phosphorylation at one or both of these residues is critical for activation of NF-kappa B .", "label": " ( protein: NF-kappa B, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: NF-kappa B, None) " }

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{ "id": "188", "sentence": "Regulation of transcription of the human erythropoietin receptor gene by proteins binding to GATA-1 and Sp1 motifs .", "label": " ( DNA: GATA-1, None) , ( DNA: human erythropoietin receptor gene, None) , ( DNA: GATA-1 and Sp1 motifs, None) , ( DNA: Sp1 motifs, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: GATA-1, None) , ( DNA: human erythropoietin receptor gene, None) , ( DNA: GATA-1 and Sp1 motifs, None) , ( DNA: Sp1 motifs, None) " }

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{ "id": "189", "sentence": "Erythropoietin ( Epo ), the primary regulator of the production of erythroid cells , acts by binding to a cell surface receptor ( EpoR ) on erythroid progenitors .", "label": " ( protein: Epo, None) , ( cell type: erythroid progenitors, None) , ( protein: cell surface receptor, None) , ( cell type: erythroid cells, None) , ( protein: EpoR, None) , ( protein: Erythropoietin, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: Epo, None) , ( cell type: erythroid progenitors, None) , ( protein: cell surface receptor, None) , ( cell type: erythroid cells, None) , ( protein: EpoR, None) , ( protein: Erythropoietin, None) " }

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{ "id": "190", "sentence": "We used deletion analysis and transfection assays with reporter gene constructs to examine the transcription control elements in the 5' flanking region of the human EpoR gene .", "label": " ( DNA: human EpoR gene, None) , ( DNA: transcription control elements, None) , ( DNA: 5#$%# flanking region, None) , ( protein: EpoR, None) , ( DNA: reporter gene constructs, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: human EpoR gene, None) , ( DNA: transcription control elements, None) , ( DNA: 5#$%# flanking region, None) , ( protein: EpoR, None) , ( DNA: reporter gene constructs, None) " }

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{ "id": "191", "sentence": "In erythroid cells most of the transcription activity was contained in a 150 bp promoter fragment with binding sites for transcription factors AP2 , Sp1 and the erythroid-specific GATA-1 .", "label": " ( DNA: binding sites, None) , ( protein: transcription factors, None) , ( DNA: 150 bp promoter, None) , ( protein: erythroid-specific GATA-1, None) , ( protein: Sp1, None) , ( cell type: erythroid cells, None) , ( protein: AP2, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: binding sites, None) , ( protein: transcription factors, None) , ( DNA: 150 bp promoter, None) , ( protein: erythroid-specific GATA-1, None) , ( protein: Sp1, None) , ( cell type: erythroid cells, None) , ( protein: AP2, None) " }

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{ "id": "192", "sentence": "The 150 bp hEpoR promoter exhibited high and low activity in erythroid OCIM1 and K562 cells , respectively, reflecting the high and low levels of constitutive hEpoR expression .", "label": " ( cell line: OCIM1, None) , ( protein: hEpoR, None) , ( DNA: 150 bp hEpoR promoter, None) , ( cell line: K562 cells, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell line: OCIM1, None) , ( protein: hEpoR, None) , ( DNA: 150 bp hEpoR promoter, None) , ( cell line: K562 cells, None) " }

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{ "id": "193", "sentence": "The GATA-1 and Sp1 binding sites in this promoter lacking a TATA sequence were necessary for a high level of transcription activation .", "label": " ( DNA: TATA sequence, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: TATA sequence, None) " }

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{ "id": "194", "sentence": "Protein-DNA binding studies suggested that Sp1 and two other CCGCCC binding proteins from erythroid and non-erythroid cells could bind to the Sp1 binding motif .", "label": " ( DNA: Sp1 binding motif, None) , ( protein: CCGCCC binding proteins, None) , ( protein: Sp1, None) , ( protein: Sp1, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( DNA: Sp1 binding motif, None) , ( protein: CCGCCC binding proteins, None) , ( protein: Sp1, None) , ( protein: Sp1, None) " }

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{ "id": "195", "sentence": "By increasing GATA-1 levels via co-transfection , we were able to transactivate the hEpoR promoter in K562 cells and non-erythroid cells , but not in the highly active OCIM1 cells , although GATA-1 mRNA levels were comparable in OCIM1 and K562 .", "label": " ( cell line: OCIM1, None) , ( RNA: GATA-1 mRNA, None) , ( protein: GATA-1, None) , ( cell type: OCIM1 cells, None) , ( cell line: K562 cells, None) , ( cell type: non-erythroid cells, None) , ( cell line: K562, None) , ( protein: GATA-1, None) , ( DNA: hEpoR promoter, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell line: OCIM1, None) , ( RNA: GATA-1 mRNA, None) , ( protein: GATA-1, None) , ( cell type: OCIM1 cells, None) , ( cell line: K562 cells, None) , ( cell type: non-erythroid cells, None) , ( cell line: K562, None) , ( protein: GATA-1, None) , ( DNA: hEpoR promoter, None) " }

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{ "id": "196", "sentence": "Interestingly, when we mutated the Sp1 site , resulting in a marked decrease in hEpoR promoter activity , we could restore transactivation by increasing GATA-1 levels in OCIM1 cells .", "label": " ( cell type: OCIM1 cells, None) , ( protein: GATA-1, None) , ( protein: Sp1, None) , ( DNA: Sp1 site, None) , ( DNA: hEpoR promoter, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( cell type: OCIM1 cells, None) , ( protein: GATA-1, None) , ( protein: Sp1, None) , ( DNA: Sp1 site, None) , ( DNA: hEpoR promoter, None) " }

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{ "id": "197", "sentence": "These data suggest that while GATA-1 can transactivate the EpoR promoter , the level of hEpoR gene expression does not depend on GATA-1 alone.", "label": " ( protein: GATA-1, None) , ( protein: EpoR, None) , ( DNA: EpoR promoter, None) , ( protein: GATA-1, None) , ( DNA: hEpoR gene, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: GATA-1, None) , ( protein: EpoR, None) , ( DNA: EpoR promoter, None) , ( protein: GATA-1, None) , ( DNA: hEpoR gene, None) " }

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{ "id": "198", "sentence": "Rather, hEpoR transcription activity depends on coordination between Sp1 and GATA-1 with other cell-specific factors , including possibly other Sp1 -like binding proteins , to provide high level, tissue-specific expression .", "label": " ( protein: hEpoR, None) , ( protein: Sp1, None) , ( protein: cell-specific factors, None) , ( protein: Sp1, None) , ( protein: GATA-1, None) , ( protein: Sp1 -like binding proteins, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: hEpoR, None) , ( protein: Sp1, None) , ( protein: cell-specific factors, None) , ( protein: Sp1, None) , ( protein: GATA-1, None) , ( protein: Sp1 -like binding proteins, None) " }

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{ "id": "199", "sentence": "Overexpression of DR-nm23 , a protein encoded by a member of the nm23 gene family , inhibits granulocyte differentiation and induces apoptosis in 32Dc13 myeloid cells .", "label": " ( protein: DR-nm23, None) , ( cell line: 32Dc13 myeloid cells, None) , ( DNA: nm23 gene family, None) , ( cell type: granulocyte, None) ", "instruction": "Locate the role in the text that participated in the event based on the event type and return it in the event list. \n Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . \nText: {0} \nAnswer:", "ground_truth": " ( protein: DR-nm23, None) , ( cell line: 32Dc13 myeloid cells, None) , ( DNA: nm23 gene family, None) , ( cell type: granulocyte, None) " }