""" Geneformer in silico perturber. Usage: from geneformer import InSilicoPerturber isp = InSilicoPerturber(perturb_type="delete", perturb_rank_shift=None, genes_to_perturb="all", combos=0, anchor_gene=None, model_type="Pretrained", num_classes=0, emb_mode="cell", cell_emb_style="mean_pool", filter_data={"cell_type":["cardiomyocyte"]}, cell_states_to_model={"disease":(["dcm"],["ctrl"],["hcm"])}, max_ncells=None, emb_layer=-1, forward_batch_size=100, nproc=4, save_raw_data=False) isp.perturb_data("path/to/model", "path/to/input_data", "path/to/output_directory", "output_prefix") """ # imports import itertools as it import logging import pickle import seaborn as sns; sns.set() import torch from collections import defaultdict from datasets import Dataset, load_from_disk from tqdm.notebook import trange from transformers import BertForMaskedLM, BertForTokenClassification, BertForSequenceClassification from .tokenizer import TOKEN_DICTIONARY_FILE logger = logging.getLogger(__name__) def quant_layers(model): layer_nums = [] for name, parameter in model.named_parameters(): if "layer" in name: layer_nums += [name.split("layer.")[1].split(".")[0]] return int(max(layer_nums))+1 def flatten_list(megalist): return [item for sublist in megalist for item in sublist] def forward_pass_single_cell(model, example_cell, layer_to_quant): example_cell.set_format(type="torch") input_data = example_cell["input_ids"] with torch.no_grad(): outputs = model( input_ids = input_data.to("cuda") ) emb = torch.squeeze(outputs.hidden_states[layer_to_quant]) del outputs return emb def perturb_emb_by_index(emb, indices): mask = torch.ones(emb.numel(), dtype=torch.bool) mask[indices] = False return emb[mask] def delete_index(example): indexes = example["perturb_index"] if len(indexes)>1: indexes = flatten_list(indexes) for index in sorted(indexes, reverse=True): del example["input_ids"][index] return example def overexpress_index(example): indexes = example["perturb_index"] if len(indexes)>1: indexes = flatten_list(indexes) for index in sorted(indexes, reverse=True): example["input_ids"].insert(0, example["input_ids"].pop(index)) return example def make_perturbation_batch(example_cell, perturb_type, tokens_to_perturb, anchor_token, combo_lvl, num_proc): if tokens_to_perturb == "all": if perturb_type in ["overexpress","activate"]: range_start = 1 elif perturb_type in ["delete","inhibit"]: range_start = 0 indices_to_perturb = [[i] for i in range(range_start,example_cell["length"][0])] elif combo_lvl>0 and (anchor_token is not None): example_input_ids = example_cell["input_ids "][0] anchor_index = example_input_ids.index(anchor_token[0]) indices_to_perturb = [sorted([anchor_index,i]) if i!=anchor_index else None for i in range(example_cell["length"][0])] indices_to_perturb = [item for item in indices_to_perturb if item is not None] else: example_input_ids = example_cell["input_ids"][0] indices_to_perturb = [[example_input_ids.index(token)] if token in example_input_ids else None for token in tokens_to_perturb] indices_to_perturb = [item for item in indices_to_perturb if item is not None] # create all permutations of combo_lvl of modifiers from tokens_to_perturb if combo_lvl>0 and (anchor_token is None): if tokens_to_perturb != "all": if len(tokens_to_perturb) == combo_lvl+1: indices_to_perturb = [list(x) for x in it.combinations(indices_to_perturb, combo_lvl+1)] else: all_indices = [[i] for i in range(example_cell["length"][0])] all_indices = [index for index in all_indices if index not in indices_to_perturb] indices_to_perturb = [[[j for i in indices_to_perturb for j in i], x] for x in all_indices] length = len(indices_to_perturb) perturbation_dataset = Dataset.from_dict({"input_ids": example_cell["input_ids"]*length, "perturb_index": indices_to_perturb}) if length<400: num_proc_i = 1 else: num_proc_i = num_proc if perturb_type == "delete": perturbation_dataset = perturbation_dataset.map(delete_index, num_proc=num_proc_i) elif perturb_type == "overexpress": perturbation_dataset = perturbation_dataset.map(overexpress_index, num_proc=num_proc_i) return perturbation_dataset, indices_to_perturb # original cell emb removing the respective perturbed gene emb def make_comparison_batch(original_emb, indices_to_perturb): all_embs_list = [] for indices in indices_to_perturb: emb_list = [] start = 0 if len(indices)>1 and isinstance(indices[0],list): indices = flatten_list(indices) for i in sorted(indices): emb_list += [original_emb[start:i]] start = i+1 emb_list += [original_emb[start:]] all_embs_list += [torch.cat(emb_list)] return torch.stack(all_embs_list) # perturbed cell emb removing the activated/overexpressed/inhibited gene emb # so that only non-perturbed gene embeddings are compared to each other # in original or perturbed context def make_perturbed_remainder_batch(emb_batch, indices_to_remove): if type(indices_to_remove) == int: indices_to_keep = [i for i in range(emb_batch.size()[1])] indices_to_keep.pop(indices_to_remove) perturbed_remainder_batch = torch.stack([emb[indices_to_keep,:] for emb in emb_batch]) elif type(indices_to_remove) == list: perturbed_remainder_batch = torch.stack([make_comparison_batch(emb_batch[i],indices_to_remove[i]) for i in range(len(emb_batch))]) return perturbed_remainder_batch # average embedding position of goal cell states def get_cell_state_avg_embs(model, filtered_input_data, cell_states_to_model, layer_to_quant, token_dictionary, forward_batch_size, num_proc): possible_states = [value[0]+value[1]+value[2] for value in cell_states_to_model.values()][0] state_embs_dict = dict() for possible_state in possible_states: state_embs_list = [] def filter_states(example): return example[list(cell_states_to_model.keys())[0]] in [possible_state] filtered_input_data_state = filtered_input_data.filter(filter_states, num_proc=num_proc) total_batch_length = len(filtered_input_data_state) if ((total_batch_length-1)/forward_batch_size).is_integer(): forward_batch_size = forward_batch_size-1 max_len = max(filtered_input_data_state["length"]) for i in range(0, total_batch_length, forward_batch_size): max_range = min(i+forward_batch_size, total_batch_length) state_minibatch = filtered_input_data_state.select([i for i in range(i, max_range)]) state_minibatch.set_format(type="torch") input_data_minibatch = state_minibatch["input_ids"] input_data_minibatch = pad_tensor_list(input_data_minibatch, max_len, token_dictionary) with torch.no_grad(): outputs = model( input_ids = input_data_minibatch.to("cuda") ) state_embs_i = outputs.hidden_states[layer_to_quant] state_embs_list += [state_embs_i] del outputs del state_minibatch del input_data_minibatch del state_embs_i torch.cuda.empty_cache() state_embs_stack = torch.cat(state_embs_list) avg_state_emb = torch.mean(state_embs_stack,dim=[0,1],keepdim=True) state_embs_dict[possible_state] = avg_state_emb return state_embs_dict # quantify cosine similarity of perturbed vs original or alternate states def quant_cos_sims(model, perturb_type, perturbation_batch, forward_batch_size, layer_to_quant, original_emb, indices_to_perturb, cell_states_to_model, state_embs_dict): cos = torch.nn.CosineSimilarity(dim=2) total_batch_length = len(perturbation_batch) if ((total_batch_length-1)/forward_batch_size).is_integer(): forward_batch_size = forward_batch_size-1 if cell_states_to_model is None: comparison_batch = make_comparison_batch(original_emb, indices_to_perturb) cos_sims = [] else: possible_states = [value[0]+value[1]+value[2] for value in cell_states_to_model.values()][0] cos_sims_vs_alt_dict = dict(zip(possible_states,[[] for i in range(len(possible_states))])) for i in range(0, total_batch_length, forward_batch_size): max_range = min(i+forward_batch_size, total_batch_length) perturbation_minibatch = perturbation_batch.select([i for i in range(i, max_range)]) perturbation_minibatch.set_format(type="torch") input_data_minibatch = perturbation_minibatch["input_ids"] with torch.no_grad(): outputs = model( input_ids = input_data_minibatch.to("cuda") ) del input_data_minibatch del perturbation_minibatch # cosine similarity between original emb and batch items if len(indices_to_perturb)>1: minibatch_emb = torch.squeeze(outputs.hidden_states[layer_to_quant]) else: minibatch_emb = outputs.hidden_states[layer_to_quant] if cell_states_to_model is None: minibatch_comparison = comparison_batch[i:max_range] if perturb_type == "overexpress": index_to_remove = 0 minibatch_emb = make_perturbed_remainder_batch(minibatch_emb, index_to_remove) # elif (perturb_type == "inhibit") or (perturb_type == "activate"): # index_to_remove = placeholder # minibatch_emb = make_perturbed_remainder_batch(minibatch_emb, index_to_remove) cos_sims += [cos(minibatch_emb, minibatch_comparison).to("cpu")] elif cell_states_to_model is not None: for state in possible_states: cos_sims_vs_alt_dict[state] += cos_sim_shift(original_emb, minibatch_emb, state_embs_dict[state]) del outputs del minibatch_emb if cell_states_to_model is None: del minibatch_comparison torch.cuda.empty_cache() if cell_states_to_model is None: cos_sims_stack = torch.cat(cos_sims) return cos_sims_stack else: for state in possible_states: cos_sims_vs_alt_dict[state] = torch.cat(cos_sims_vs_alt_dict[state]) return cos_sims_vs_alt_dict # calculate cos sim shift of perturbation with respect to origin and alternative cell def cos_sim_shift(original_emb, minibatch_emb, alt_emb): cos = torch.nn.CosineSimilarity(dim=2) original_emb = torch.mean(original_emb,dim=0,keepdim=True)[None, :] alt_emb = alt_emb[None, None, :] origin_v_end = cos(original_emb,alt_emb) perturb_v_end = cos(torch.mean(minibatch_emb,dim=1,keepdim=True),alt_emb) return [(perturb_v_end-origin_v_end).to("cpu")] # pad list of tensors and convert to tensor def pad_tensor_list(tensor_list, dynamic_or_constant, token_dictionary): pad_token_id = token_dictionary.get("") # Determine maximum tensor length if dynamic_or_constant == "dynamic": max_len = max([tensor.squeeze().numel() for tensor in tensor_list]) elif type(dynamic_or_constant) == int: max_len = dynamic_or_constant else: logger.warning( "If padding style is constant, must provide integer value. " \ "Setting padding to max input size 2048.") # pad all tensors to maximum length tensor_list = [torch.nn.functional.pad(tensor, pad=(0, max_len - tensor.numel()), mode='constant', value=pad_token_id) for tensor in tensor_list] # return stacked tensors return torch.stack(tensor_list) class InSilicoPerturber: valid_option_dict = { "perturb_type": {"delete","overexpress","inhibit","activate"}, "perturb_rank_shift": {None, 1, 2, 3}, "genes_to_perturb": {"all", list}, "combos": {0, 1, 2}, "anchor_gene": {None, str}, "model_type": {"Pretrained","GeneClassifier","CellClassifier"}, "num_classes": {int}, "emb_mode": {"cell","cell_and_gene"}, "cell_emb_style": {"mean_pool"}, "filter_data": {None, dict}, "cell_states_to_model": {None, dict}, "max_ncells": {None, int}, "emb_layer": {-1, 0}, "forward_batch_size": {int}, "nproc": {int}, "save_raw_data": {False, True}, } def __init__( self, perturb_type="delete", perturb_rank_shift=None, genes_to_perturb="all", combos=0, anchor_gene=None, model_type="Pretrained", num_classes=0, emb_mode="cell", cell_emb_style="mean_pool", filter_data=None, cell_states_to_model=None, max_ncells=None, emb_layer=-1, forward_batch_size=100, nproc=4, save_raw_data=False, token_dictionary_file=TOKEN_DICTIONARY_FILE, ): """ Initialize in silico perturber. Parameters ---------- perturb_type : {"delete","overexpress","inhibit","activate"} Type of perturbation. "delete": delete gene from rank value encoding "overexpress": move gene to front of rank value encoding "inhibit": move gene to lower quartile of rank value encoding "activate": move gene to higher quartile of rank value encoding perturb_rank_shift : None, {1,2,3} Number of quartiles by which to shift rank of gene. For example, if perturb_type="activate" and perturb_rank_shift=1: genes in 4th quartile will move to middle of 3rd quartile. genes in 3rd quartile will move to middle of 2nd quartile. genes in 2nd quartile will move to middle of 1st quartile. genes in 1st quartile will move to front of rank value encoding. For example, if perturb_type="inhibit" and perturb_rank_shift=2: genes in 1st quartile will move to middle of 3rd quartile. genes in 2nd quartile will move to middle of 4th quartile. genes in 3rd or 4th quartile will move to bottom of rank value encoding. genes_to_perturb : "all", list Default is perturbing each gene detected in each cell in the dataset. Otherwise, may provide a list of ENSEMBL IDs of genes to perturb. combos : {0,1,2} Whether to perturb genes individually (0), in pairs (1), or in triplets (2). anchor_gene : None, str ENSEMBL ID of gene to use as anchor in combination perturbations. For example, if combos=1 and anchor_gene="ENSG00000148400": anchor gene will be perturbed in combination with each other gene. model_type : {"Pretrained","GeneClassifier","CellClassifier"} Whether model is the pretrained Geneformer or a fine-tuned gene or cell classifier. num_classes : int If model is a gene or cell classifier, specify number of classes it was trained to classify. For the pretrained Geneformer model, number of classes is 0 as it is not a classifier. emb_mode : {"cell","cell_and_gene"} Whether to output impact of perturbation on cell and/or gene embeddings. cell_emb_style : "mean_pool" Method for summarizing cell embeddings. Currently only option is mean pooling of gene embeddings for given cell. filter_data : None, dict Default is to use all input data for in silico perturbation study. Otherwise, dictionary specifying .dataset column name and list of values to filter by. cell_states_to_model: None, dict Cell states to model if testing perturbations that achieve goal state change. Single-item dictionary with key being cell attribute (e.g. "disease"). Value is tuple of three lists indicating start state, goal end state, and alternate possible end states. max_ncells : None, int Maximum number of cells to test. If None, will test all cells. emb_layer : {-1, 0} Embedding layer to use for quantification. -1: 2nd to last layer (recommended for pretrained Geneformer) 0: last layer (recommended for cell classifier fine-tuned for disease state) forward_batch_size : int Batch size for forward pass. nproc : int Number of CPU processes to use. save_raw_data: {False,True} Whether to save raw perturbation data for each gene/cell. token_dictionary_file : Path Path to pickle file containing token dictionary (Ensembl ID:token). """ self.perturb_type = perturb_type self.perturb_rank_shift = perturb_rank_shift self.genes_to_perturb = genes_to_perturb self.combos = combos self.anchor_gene = anchor_gene self.model_type = model_type self.num_classes = num_classes self.emb_mode = emb_mode self.cell_emb_style = cell_emb_style self.filter_data = filter_data self.cell_states_to_model = cell_states_to_model self.max_ncells = max_ncells self.emb_layer = emb_layer self.forward_batch_size = forward_batch_size self.nproc = nproc self.save_raw_data = save_raw_data self.validate_options() # load token dictionary (Ensembl IDs:token) with open(token_dictionary_file, "rb") as f: self.gene_token_dict = pickle.load(f) if anchor_gene is None: self.anchor_token = None else: self.anchor_token = self.gene_token_dict[self.anchor_gene] if genes_to_perturb == "all": self.tokens_to_perturb = "all" else: self.tokens_to_perturb = [self.gene_token_dict[gene] for gene in self.genes_to_perturb] def validate_options(self): # first disallow options under development if self.perturb_type in ["inhibit", "activate"]: logger.error( f"In silico inhibition and activation currently under developemnt. " \ f"Current valid options for 'perturb_type': 'delete' or 'overexpress'" ) raise # confirm arguments are within valid options and compatible with each other for attr_name,valid_options in self.valid_option_dict.items(): attr_value = self.__dict__[attr_name] if type(attr_value) not in {list, dict}: if attr_value in valid_options: continue valid_type = False for option in valid_options: if (option in [int,list,dict]) and isinstance(attr_value, option): valid_type = True break if valid_type: continue logger.error( f"Invalid option for {attr_name}. " \ f"Valid options for {attr_name}: {valid_options}" ) raise if self.perturb_type in ["delete","overexpress"]: if self.perturb_rank_shift is not None: if self.perturb_type == "delete": logger.warning( "perturb_rank_shift set to None. " \ "If perturb type is delete then gene is deleted entirely " \ "rather than shifted by quartile") elif self.perturb_type == "overexpress": logger.warning( "perturb_rank_shift set to None. " \ "If perturb type is overexpress then gene is moved to front " \ "of rank value encoding rather than shifted by quartile") self.perturb_rank_shift = None if (self.anchor_gene is not None) and (self.emb_mode == "cell_and_gene"): self.emb_mode = "cell" logger.warning( "emb_mode set to 'cell'. " \ "Currently, analysis with anchor gene " \ "only outputs effect on cell embeddings.") if self.cell_states_to_model is not None: if (len(self.cell_states_to_model.items()) == 1): for key,value in self.cell_states_to_model.items(): if (len(value) == 3) and isinstance(value, tuple): if isinstance(value[0],list) and isinstance(value[1],list) and isinstance(value[2],list): if len(value[0]) == 1 and len(value[1]) == 1: all_values = value[0]+value[1]+value[2] if len(all_values) == len(set(all_values)): continue else: logger.error( "Cell states to model must be a single-item dictionary with " \ "key being cell attribute (e.g. 'disease') and value being " \ "tuple of three lists indicating start state, goal end state, and alternate possible end states. " \ "Values should all be unique. " \ "For example: {'disease':(['dcm'],['ctrl'],['hcm'])}") raise if self.anchor_gene is not None: self.anchor_gene = None logger.warning( "anchor_gene set to None. " \ "Currently, anchor gene not available " \ "when modeling multiple cell states.") if self.perturb_type in ["inhibit","activate"]: if self.perturb_rank_shift is None: logger.error( "If perturb type is inhibit or activate then " \ "quartile to shift by must be specified.") raise if self.filter_data is not None: for key,value in self.filter_data.items(): if type(value) != list: self.filter_data[key] = [value] logger.warning( "Values in filter_data dict must be lists. " \ f"Changing {key} value to list ([{value}]).") def perturb_data(self, model_directory, input_data_file, output_directory, output_prefix): """ Perturb genes in input data and save as results in output_directory. Parameters ---------- model_directory : Path Path to directory containing model input_data_file : Path Path to directory containing .dataset inputs output_directory : Path Path to directory where perturbation data will be saved as .csv output_prefix : str Prefix for output .dataset """ filtered_input_data = self.load_and_filter(input_data_file) model = self.load_model(model_directory) layer_to_quant = quant_layers(model)+self.emb_layer if self.cell_states_to_model is None: state_embs_dict = None else: # get dictionary of average cell state embeddings for comparison state_embs_dict = get_cell_state_avg_embs(model, filtered_input_data, self.cell_states_to_model, layer_to_quant, self.gene_token_dict, self.forward_batch_size, self.nproc) self.in_silico_perturb(model, filtered_input_data, layer_to_quant, state_embs_dict, output_directory, output_prefix) # if self.save_raw_data is False: # # delete intermediate dictionaries # output_dir = os.listdir(output_directory) # for output_file in output_dir: # if output_file.endswith("_raw.pickle"): # os.remove(os.path.join(output_directory, output_file)) # load data and filter by defined criteria def load_and_filter(self, input_data_file): data = load_from_disk(input_data_file) if self.filter_data is not None: for key,value in self.filter_data.items(): def filter_data_by_criteria(example): return example[key] in value data = data.filter(filter_data_by_criteria, num_proc=self.nproc) if len(data) == 0: logger.error( "No cells remain after filtering. Check filtering criteria.") raise data_shuffled = data.shuffle(seed=42) num_cells = len(data_shuffled) # if max number of cells is defined, then subsample to this max number if self.max_ncells != None: num_cells = min(self.max_ncells,num_cells) data_subset = data_shuffled.select([i for i in range(num_cells)]) # sort dataset with largest cell first to encounter any memory errors earlier data_sorted = data_subset.sort("length",reverse=True) return data_sorted # load model to GPU def load_model(self, model_directory): if self.model_type == "Pretrained": model = BertForMaskedLM.from_pretrained(model_directory, output_hidden_states=True, output_attentions=False) elif self.model_type == "GeneClassifier": model = BertForTokenClassification.from_pretrained(model_directory, num_labels=self.num_classes, output_hidden_states=True, output_attentions=False) elif self.model_type == "CellClassifier": model = BertForSequenceClassification.from_pretrained(model_directory, num_labels=self.num_classes, output_hidden_states=True, output_attentions=False) # put the model in eval mode for fwd pass model.eval() model = model.to("cuda:0") return model # determine effect of perturbation on other genes def in_silico_perturb(self, model, filtered_input_data, layer_to_quant, state_embs_dict, output_directory, output_prefix): output_path_prefix = f"{output_directory}in_silico_{self.perturb_type}_{output_prefix}_dict_1Kbatch" # filter dataset for cells that have tokens to be perturbed if self.anchor_token is not None: def if_has_tokens_to_perturb(example): return (len(set(example["input_ids"]).intersection(self.anchor_token))==len(self.anchor_token)) filtered_input_data = filtered_input_data.filter(if_has_tokens_to_perturb, num_proc=self.nproc) logger.info(f"# cells with anchor gene: {len(filtered_input_data)}") if self.tokens_to_perturb != "all": def if_has_tokens_to_perturb(example): return (len(set(example["input_ids"]).intersection(self.tokens_to_perturb))>self.combos) filtered_input_data = filtered_input_data.filter(if_has_tokens_to_perturb, num_proc=self.nproc) cos_sims_dict = defaultdict(list) pickle_batch = -1 for i in trange(len(filtered_input_data)): example_cell = filtered_input_data.select([i]) original_emb = forward_pass_single_cell(model, example_cell, layer_to_quant) gene_list = torch.squeeze(example_cell["input_ids"]) # reset to original type to prevent downstream issues due to forward_pass_single_cell modifying as torch format in place example_cell = filtered_input_data.select([i]) if self.anchor_token is None: for combo_lvl in range(self.combos+1): perturbation_batch, indices_to_perturb = make_perturbation_batch(example_cell, self.perturb_type, self.tokens_to_perturb, self.anchor_token, combo_lvl, self.nproc) cos_sims_data = quant_cos_sims(model, self.perturb_type, perturbation_batch, self.forward_batch_size, layer_to_quant, original_emb, indices_to_perturb, self.cell_states_to_model, state_embs_dict) if self.cell_states_to_model is None: # update cos sims dict # key is tuple of (perturbed_gene, affected_gene) # or (perturbed_gene, "cell_emb") for avg cell emb change cos_sims_data = cos_sims_data.to("cuda") for j in range(cos_sims_data.shape[0]): if self.genes_to_perturb != "all": j_index = torch.tensor(indices_to_perturb[j]) if j_index.shape[0]>1: j_index = torch.squeeze(j_index) else: j_index = torch.tensor([j]) perturbed_gene = torch.index_select(gene_list, 0, j_index) if perturbed_gene.shape[0]==1: perturbed_gene = perturbed_gene.item() elif perturbed_gene.shape[0]>1: perturbed_gene = tuple(perturbed_gene.tolist()) cell_cos_sim = torch.mean(cos_sims_data[j]).item() cos_sims_dict[(perturbed_gene, "cell_emb")] += [cell_cos_sim] # not_j_index = list(set(i for i in range(gene_list.shape[0])).difference(j_index)) # gene_list_j = torch.index_select(gene_list, 0, j_index) if self.emb_mode == "cell_and_gene": for k in range(cos_sims_data.shape[1]): cos_sim_value = cos_sims_data[j][k] affected_gene = gene_list[k].item() cos_sims_dict[(perturbed_gene, affected_gene)] += [cos_sim_value.item()] else: # update cos sims dict # key is tuple of (perturbed_gene, "cell_emb") # value is list of tuples of cos sims for cell_states_to_model origin_state_key = [value[0] for value in self.cell_states_to_model.values()][0][0] cos_sims_origin = cos_sims_data[origin_state_key] for j in range(cos_sims_origin.shape[0]): if (self.genes_to_perturb != "all") or (combo_lvl>0): j_index = torch.tensor(indices_to_perturb[j]) if j_index.shape[0]>1: j_index = torch.squeeze(j_index) else: j_index = torch.tensor([j]) perturbed_gene = torch.index_select(gene_list, 0, j_index) if perturbed_gene.shape[0]==1: perturbed_gene = perturbed_gene.item() elif perturbed_gene.shape[0]>1: perturbed_gene = tuple(perturbed_gene.tolist()) data_list = [] for data in list(cos_sims_data.values()): data_item = data.to("cuda") cell_data = torch.mean(data_item[j]).item() data_list += [cell_data] cos_sims_dict[(perturbed_gene, "cell_emb")] += [tuple(data_list)] elif self.anchor_token is not None: perturbation_batch, indices_to_perturb = make_perturbation_batch(example_cell, self.perturb_type, self.tokens_to_perturb, None, # first run without anchor token to test individual gene perturbations 0, self.nproc) cos_sims_data = quant_cos_sims(model, self.perturb_type, perturbation_batch, self.forward_batch_size, layer_to_quant, original_emb, indices_to_perturb, self.cell_states_to_model, state_embs_dict) cos_sims_data = cos_sims_data.to("cuda") combo_perturbation_batch, combo_indices_to_perturb = make_perturbation_batch(example_cell, self.perturb_type, self.tokens_to_perturb, self.anchor_token, 1, self.nproc) combo_cos_sims_data = quant_cos_sims(model, self.perturb_type, combo_perturbation_batch, self.forward_batch_size, layer_to_quant, original_emb, combo_indices_to_perturb, self.cell_states_to_model, state_embs_dict) combo_cos_sims_data = combo_cos_sims_data.to("cuda") # update cos sims dict # key is tuple of (perturbed_gene, "cell_emb") for avg cell emb change anchor_index = example_cell["input_ids"][0].index(self.anchor_token[0]) anchor_cell_cos_sim = torch.mean(cos_sims_data[anchor_index]).item() non_anchor_indices = [k for k in range(cos_sims_data.shape[0]) if k != anchor_index] cos_sims_data = cos_sims_data[non_anchor_indices,:] for j in range(cos_sims_data.shape[0]): if j